protein-translation books

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Cloning, Gene Expression, and Protein Purification: Experimental Procedures and Process Rationale

by: Charles Hardin
publisher: Oxford University Press, USA, published: 2001-03-01
ASIN: 0195132947
EAN: 9780195132946
sales rank: 1444809
On the forefront of modern scientific innovation, Cloning, Gene Expression and Protein Purification: Experimental Procedures and Process Rationale effectively doubles as a laboratory manual for students and a reference book for professional researchers. Designed for advanced undergraduate and beginning graduate students in molecular biology, this unique combination lecture/laboratory resource presents detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing its encoded protein, and purifying and characterizing the protein's basic physical properties. This manageable volume includes both theoretical background and practical procedures and is structured around twenty experiments that demonstrate how to prepare, manipulate, and analyze plasmids, produce fusion proteins in bacteria, and purify these proteins based on unique chemical properties or substrate affinities. The book describes advanced topics such as the use of antibodies and the techniques developed to transform their structures, as well as combinatorial approaches designed to manipulate the structure and functions of proteins and nucleic acids. Supplemental literature provides a variety of theoretical explanations encouraging a more intuitive understanding of the experimental mechanisms and behaviors of the chemical participants, while also giving students the tools needed to become "capable proactive researchers." Features:
ï¿½ Emphasizes electrophoresis, Southern and Western blotting, and combinatorial techniques
ï¿½ Defines clear reaction mechanisms; stipulates the functions of reagents; and helps students think about the precise consequences of solution and procedural manipulations
ï¿½ Discusses fluorophores, and solvent effects on protein structure
ï¿½ Characterizes plasmids, cDNAs, and antibody probes (available from ATCC) in research literature
ï¿½ Includes carefully selected primary source research literature and articles from current vendor literature
ï¿½ Contains a glossary of unfamiliar phrases and jargon; important summary statements and conclusions are italicized
ï¿½ Provides an alphabetized list of common reagents for rapid reference
ï¿½ Offers an extensive index of concepts and terms
ï¿½ Categorizes helpful and distinctive information into five types of supplemental literature: Innovation/ Insight, Theory/Principle, Process Rationale, Vendor Literature, and Alternative Approaches

Stories of the Invisible: A Guided Tour of Molecules

by: Philip Ball
publisher: Oxford University Press, USA, published: 2002-12-12
ASIN: 0192803174
EAN: 9780192803177
sales rank: 1444999
If atoms are letters, writes Philip Ball, then molecules are words. And through these words, scientists have uncovered many fascinating stories of the physical world. In Stories of the Invisible, Ball has compiled a cornucopia of tales spun by these intriguing, invisible words.
The book takes us on a tour of a world few of us knew existed. The author describes the remarkable molecular structure of spider's silk--a material that is pound for pound much stronger than steel--and shows how the Kevlar fibers in bulletproof vests were invented by imitating the alignment of molecules found in the spider's amazing thread. We also learn about the protein molecules that create movement, without which bacteria would be immobile, cells could not divide, there would be no reproduction and therefore no life.
Today we can invent molecules that can cure viral infections, store information, or help hold bridges together. But more importantly, Ball provides a fresh perspective on the future of molecular science, revealing how researchers are promising to reinvent chemistry as the central creative science of the 21st century.

From Gene to Protein: Translation into Biotechnology (Miami winter symposia)

by: Fazal Ahmad
publisher: Academic Pr, published: 1982-11
ASIN: 0120455609
EAN: 9780120455607
sales rank: 9727365

Translational Control in Biology And Medicine (CSH Monograph)

publisher: Cold Spring Harbor Laboratory Press, published: 2006-12-30
ASIN: 0879697679
EAN: 9780879697679
sales rank: 2310744
The new edition of this successful monograph has been both updated and broadened. Since the previous (second) edition was published in 2000, the structures of the bacterial and eukaryotic ribosomes have been published, advancing our basic understanding of translation and mechanisms involving protein and RNA regulators. In addition, as the title indicates, this edition has a new focus on the role of translational control in human development and disease. This book, with 30 chapters written by experts in the field, is essential reading for anyone interested in the process of translation, its regulation, and how its failure can be the cause of disease. Related Titles from the Publisher Translational Control of Gene Expression

Protein Expression: A Practical Approach (Practical Approach Series)

publisher: Oxford University Press, USA, published: 1999-07-29
ASIN: 0199636230
EAN: 9780199636235
sales rank: 3666870
Part of a mini-series on the synthesis and processing of proteins, this volume details the expression of cloned DNA and RNA templates in all of the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Sacchoromyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. It also covers cell-free systems of both eukaryotes and prokaryotes, including prokaryotic systems that offer coupled transcription-translation. The volume concludes with a chapter on monitoring protein expression.

Reviews of Physiology, Biochemistry and Pharmacology 155

publisher: Springer, published: 2005-12-22
ASIN: 3540281924
EAN: 9783540281924
sales rank: 5110613
Contents S. Rospert, M. Rakwalska and Y. Dubaquie Polypeptide chain termination and stop codon readthrough on eukaryotic ribosomes A.S. Verkman Novel roles of aquaporins revealed by phenotype analysis of knockout mice M.J. Cismowski and S.M. Lanier Activation of heterotrimeric G-proteins independent of a G-protein coupled receptor and the implications for signal processing

In Vitro Transcription and Translation Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2007-05-03
ASIN: 1588295583
EAN: 9781588295583
sales rank: 3958693
A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. Completely updated with new chapters and topics, there is in-depth analysis of current technologies and applications of in-vitro transcriptions and translations systems. Detailed protocols for protein production using different in vitro transcription and translation systems are included.

In Vitro Transcription and Translation Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-12-09
ASIN: 1617376418
EAN: 9781617376412
sales rank: 3947986
A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. Completely updated with new chapters and topics, there is in-depth analysis of current technologies and applications of in-vitro transcriptions and translations systems. Detailed protocols for protein production using different in vitro transcription and translation systems are included.

Translation In Eukaryotes (Telford Press)

by: Hans Trachsel
publisher: CRC Press, published: 1991-07-24
ASIN: 0849388163
EAN: 9780849388163
sales rank: 5499079
This book presents an up-to-date review of the mechanisms and regulation of translation in eukaryotes. Topics covered include the basic biochemical reactions of translation initiation, elongation and termination, and the regulation of these reactions under different physiological conditions and in virus-infected cells. The book belongs on the shelf of everyone interested in translation in eukaryotes, including students and researchers requiring comprehensive overviews of most aspects of translation and instructors who want to cover these topics at an advanced level.

Manufacturing of Pharmaceutical Proteins

by: Stefan Behme
publisher: Wiley-VCH, published: 2009-02-24
ASIN: 3527324445
EAN: 9783527324446
sales rank: 291833
This comprehensive introduction covers all aspects of biopharmaceutical manufacturing, including technology and facilities, GMP and regulatory issues, as well as costing and timeline aspects for supply chain implementation.
Written by a leading expert at one of the largest pharmaceutical companies worldwide, this practical text is aimed at a wide audience ranging from libraries, biotech companies to students and technicians planning to enter biopharmaceutical manufacturing. In addition, it is well suited for academic teaching as well as internal training within larger biotech or pharmaceutical companies.

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	Cloning, Gene Expression, and Protein Purification: Experimental Procedures and Process Rationale by: Charles Hardin publisher: Oxford University Press, USA, published: 2001-03-01 ASIN: 0195132947 EAN: 9780195132946 sales rank: 1444809 On the forefront of modern scientific innovation, Cloning, Gene Expression and Protein Purification: Experimental Procedures and Process Rationale effectively doubles as a laboratory manual for students and a reference book for professional researchers. Designed for advanced undergraduate and beginning graduate students in molecular biology, this unique combination lecture/laboratory resource presents detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing its encoded protein, and purifying and characterizing the protein's basic physical properties. This manageable volume includes both theoretical background and practical procedures and is structured around twenty experiments that demonstrate how to prepare, manipulate, and analyze plasmids, produce fusion proteins in bacteria, and purify these proteins based on unique chemical properties or substrate affinities. The book describes advanced topics such as the use of antibodies and the techniques developed to transform their structures, as well as combinatorial approaches designed to manipulate the structure and functions of proteins and nucleic acids. Supplemental literature provides a variety of theoretical explanations encouraging a more intuitive understanding of the experimental mechanisms and behaviors of the chemical participants, while also giving students the tools needed to become "capable proactive researchers." Features: ï¿½ Emphasizes electrophoresis, Southern and Western blotting, and combinatorial techniques ï¿½ Defines clear reaction mechanisms; stipulates the functions of reagents; and helps students think about the precise consequences of solution and procedural manipulations ï¿½ Discusses fluorophores, and solvent effects on protein structure ï¿½ Characterizes plasmids, cDNAs, and antibody probes (available from ATCC) in research literature ï¿½ Includes carefully selected primary source research literature and articles from current vendor literature ï¿½ Contains a glossary of unfamiliar phrases and jargon; important summary statements and conclusions are italicized ï¿½ Provides an alphabetized list of common reagents for rapid reference ï¿½ Offers an extensive index of concepts and terms ï¿½ Categorizes helpful and distinctive information into five types of supplemental literature: Innovation/ Insight, Theory/Principle, Process Rationale, Vendor Literature, and Alternative Approaches
	Stories of the Invisible: A Guided Tour of Molecules by: Philip Ball publisher: Oxford University Press, USA, published: 2002-12-12 ASIN: 0192803174 EAN: 9780192803177 sales rank: 1444999 If atoms are letters, writes Philip Ball, then molecules are words. And through these words, scientists have uncovered many fascinating stories of the physical world. In Stories of the Invisible, Ball has compiled a cornucopia of tales spun by these intriguing, invisible words. The book takes us on a tour of a world few of us knew existed. The author describes the remarkable molecular structure of spider's silk--a material that is pound for pound much stronger than steel--and shows how the Kevlar fibers in bulletproof vests were invented by imitating the alignment of molecules found in the spider's amazing thread. We also learn about the protein molecules that create movement, without which bacteria would be immobile, cells could not divide, there would be no reproduction and therefore no life. Today we can invent molecules that can cure viral infections, store information, or help hold bridges together. But more importantly, Ball provides a fresh perspective on the future of molecular science, revealing how researchers are promising to reinvent chemistry as the central creative science of the 21st century.
	From Gene to Protein: Translation into Biotechnology (Miami winter symposia) by: Fazal Ahmad publisher: Academic Pr, published: 1982-11 ASIN: 0120455609 EAN: 9780120455607 sales rank: 9727365
	Translational Control in Biology And Medicine (CSH Monograph) publisher: Cold Spring Harbor Laboratory Press, published: 2006-12-30 ASIN: 0879697679 EAN: 9780879697679 sales rank: 2310744 The new edition of this successful monograph has been both updated and broadened. Since the previous (second) edition was published in 2000, the structures of the bacterial and eukaryotic ribosomes have been published, advancing our basic understanding of translation and mechanisms involving protein and RNA regulators. In addition, as the title indicates, this edition has a new focus on the role of translational control in human development and disease. This book, with 30 chapters written by experts in the field, is essential reading for anyone interested in the process of translation, its regulation, and how its failure can be the cause of disease. Related Titles from the Publisher Translational Control of Gene Expression
	Protein Expression: A Practical Approach (Practical Approach Series) publisher: Oxford University Press, USA, published: 1999-07-29 ASIN: 0199636230 EAN: 9780199636235 sales rank: 3666870 Part of a mini-series on the synthesis and processing of proteins, this volume details the expression of cloned DNA and RNA templates in all of the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Sacchoromyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. It also covers cell-free systems of both eukaryotes and prokaryotes, including prokaryotic systems that offer coupled transcription-translation. The volume concludes with a chapter on monitoring protein expression.
	Reviews of Physiology, Biochemistry and Pharmacology 155 publisher: Springer, published: 2005-12-22 ASIN: 3540281924 EAN: 9783540281924 sales rank: 5110613 Contents S. Rospert, M. Rakwalska and Y. Dubaquie Polypeptide chain termination and stop codon readthrough on eukaryotic ribosomes A.S. Verkman Novel roles of aquaporins revealed by phenotype analysis of knockout mice M.J. Cismowski and S.M. Lanier Activation of heterotrimeric G-proteins independent of a G-protein coupled receptor and the implications for signal processing
	In Vitro Transcription and Translation Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2007-05-03 ASIN: 1588295583 EAN: 9781588295583 sales rank: 3958693 A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. Completely updated with new chapters and topics, there is in-depth analysis of current technologies and applications of in-vitro transcriptions and translations systems. Detailed protocols for protein production using different in vitro transcription and translation systems are included.
	In Vitro Transcription and Translation Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2010-12-09 ASIN: 1617376418 EAN: 9781617376412 sales rank: 3947986 A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. Completely updated with new chapters and topics, there is in-depth analysis of current technologies and applications of in-vitro transcriptions and translations systems. Detailed protocols for protein production using different in vitro transcription and translation systems are included.
	Translation In Eukaryotes (Telford Press) by: Hans Trachsel publisher: CRC Press, published: 1991-07-24 ASIN: 0849388163 EAN: 9780849388163 sales rank: 5499079 This book presents an up-to-date review of the mechanisms and regulation of translation in eukaryotes. Topics covered include the basic biochemical reactions of translation initiation, elongation and termination, and the regulation of these reactions under different physiological conditions and in virus-infected cells. The book belongs on the shelf of everyone interested in translation in eukaryotes, including students and researchers requiring comprehensive overviews of most aspects of translation and instructors who want to cover these topics at an advanced level.
	Manufacturing of Pharmaceutical Proteins by: Stefan Behme publisher: Wiley-VCH, published: 2009-02-24 ASIN: 3527324445 EAN: 9783527324446 sales rank: 291833 This comprehensive introduction covers all aspects of biopharmaceutical manufacturing, including technology and facilities, GMP and regulatory issues, as well as costing and timeline aspects for supply chain implementation. Written by a leading expert at one of the largest pharmaceutical companies worldwide, this practical text is aimed at a wide audience ranging from libraries, biotech companies to students and technicians planning to enter biopharmaceutical manufacturing. In addition, it is well suited for academic teaching as well as internal training within larger biotech or pharmaceutical companies.