Related books on 'rna-methods'
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publisher: Humana Press, published: 2010-11-19 ASIN: 1617375683 EAN: 9781617375682 sales rank: 5851904 A collection of readily reproducible methods for the design, preparation, and use of RNAs for silencing gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. |
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publisher: Humana Press, published: 1984-12-11 ASIN: 0896030644 EAN: 9780896030640 sales rank: 4636125 |
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publisher: Humana Press, published: 2010-11-19 ASIN: 1617375551 EAN: 9781617375552 sales rank: 3713910 Due to the vital biological importance of RNA and proteins functioning together within a cell, a protocol volume describing experimental procedures to study their interactions should find a home in many laboratories. RNA-Protein Interaction Protocols, Second Edition updates, complements, and expands upon the popular first edition by providing a collection of cutting-edge techniques developed or refined in the past few years along with tried-and-true methods. The expert contributors explore the isolation and characterization of RNA-protein complexes, the analysis and measurement of RNA-protein interaction, and related novel techniques and strategies. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, RNA-Protein Interaction Protocols, Second Edition is an ideal guide for researchers continuing the study of this all-important biological partnership. |
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publisher: Academic Press, published: 2011-10-07 ASIN: 0123813204 EAN: 9780123813206 sales rank: 746684 This volume of Methods in Cell Biology is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This new volume on Disease Models and Chemical Screens, covers two rapidly emerging and compelling applications of the zebrafish. * Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls * This volume concentrates on Disease Models and Chemical Screens |
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publisher: Academic Press, published: 2007-09-13 ASIN: 0123739179 EAN: 9780123739179 sales rank: 4212880 MicroRNAs (miRNA) are tiny bits of genetic material that were unknown nearly 10 years ago but now represent an exciting field of study in biology. Upon their discovery, researchers revealed for the first time a new mechanism by which microRNA can stop the function of messenger RNA (mRNA) by literally cutting it in half, interfering with the normal function of specific messenger RNAs in gene expression. This "expression" of genes that code for essential proteins is essentially what controls whether a cell turns into a liver, lung, or brain cell, for example. Understanding what activates this process - or stops it - is a key to understanding the biological process and builds a foundation for advances in medicine and other fields. This volume in Methods in Enzymology presents valuable methods for studying MicroRNA, with three sections covering identification of MicroRNAs and their targets; Micro RNA expression, maturation and functional analysis; and MicroRNAs and disease. |
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by: Matt Carter publisher: Academic Press, published: 2009-10-06 ASIN: 0123748496 EAN: 9780123748492 sales rank: 130220 Neuroscience is by definition a multidisciplinary field: some scientists study genes and proteins at the molecular level while others study neural circuitry. A single topic such as the auditory system can be studied using techniques from genetics, imaging, biochemistry, or electrophysiology. A young scientist must learn how to read the primary literature and then develop their own experiments. This book offers that scientist an overview of mainstream research techniques, provides guidelines on how to choose one technique over another, offers tips on analyzing data, and provides a list of references for additional detailed study. This book can also assist an experienced scientist understand published studies conducted outside their own subfield. *Written by Stanford University graduate students in neuroscience to provide a "hands-on" approach for other neuroscience graduate students *Techniques within one field will be compared so that user can select best technique for their experiment *Chapters include references (key articles, books, protocols) for additional detailed study *Data Analysis boxes in each chapter help with data interpretation and offer guidelines on how best to represent results *"Walk-through" boxes guide students through the experiment step-by-step |
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publisher: Humana Press, published: 2011-04-06 ASIN: 1617790044 EAN: 9781617790041 sales rank: 1636988 With its complex and extensively regulated metabolism, the study of the RNA lifecycle demands tools that allow for the localization of RNAs to be observed either in an in situ setting or, preferably, under in vivo conditions. In RNA Detection and Visualization: Methods and Protocols, the best and brightest investigators provide an up-to-date and in-depth description of basic methods and protocols used for detecting and visualizing mRNAs in both fixed and live cells, from bacteria to mammals. For novices and experts alike, this mix of classic in situ hybridization and advanced live imaging techniques, cell fractionation and affinity purification procedures, and bioinformatics tools gives researchers the most complete and extensive array of research aids possible. As a volume written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Detection and Visualization: Methods and Protocols offers well-honed techniques in order to inspire researchers around the world to further our knowledge of the vital biological significance of RNA. |
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by: Walter K. Dodds publisher: Academic Press, published: 2002-03-19 ASIN: 0122191358 EAN: 9780122191350 sales rank: 205336 This book is a general text covering both basic and applied aspects of freshwater ecology and serves as an introduction to the study of lakes and streams. Issues of spatial and temporal scale, anthropogenic impacts, and application of current ecological concepts are covered along with ideas that are presented in more traditional limnological texts. Chapters on biodiversity, toxic chemicals, extreme and unusual habitats, and fisheries increase the breadth of material covered. The book includes an extensive glossary, questions for thought, worked examples of equations, and real-life problems. Key Features * Broad coverage of groundwaters, streams, wetlands, and lakes * Features basic scientific concepts and environmental applications throughout * Includes many figures, sidebars of fascinating applications, and biographies of practicing aquatic ecologists * Materials are presented to facilitate learning, including an extensive glossary, questions for thought, worked examples of equations, and real life problems * Written at a level understandable to most undergraduate students, with explanations of complex contemporary concepts in freshwater ecology described to promote understanding * Featuring small chapters that mainly stand alone, this book can be read in the order most suited to the specific application |
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publisher: Humana Press, published: 2010-12-09 ASIN: 1617376515 EAN: 9781617376511 sales rank: 712416 A collection of cutting-edge computational tools and experimental techniques to study how genes are regulated, and to reconstruct the regulatory networks through which various cell-types are produced. On the computational side, web-based technologies to localize genes, to access and retrieve data from microarray databases, to conduct comparative genomics, and to discover the potential "codes" in genomic DNA that may control the expression of protein-coding genes. Detailed experimental techniques described include methods for studying chromatin structure and allele-specific gene expression, methods for high-throughput analysis to characterize the transcription factor binding elements, and methods for isolating and identifying proteins that interact with DNA. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls. |
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by: Masaharu Takemura publisher: No Starch Press, published: 2009-09-04 ASIN: 1593272022 EAN: 9781593272029 sales rank: 38388 Rin and Ami have been skipping molecular biology class all semester, and Professor Moro has had enough-he's sentencing them to summer school on his private island. But they're in store for a special lesson. Using Dr. Moro's virtual reality machine to travel inside the human body, they'll get a close-up look at the fascinating world of molecular biology. Join them in The Manga Guide to Molecular Biology, and learn all about DNA, RNA, proteins, amino acids, and more. Along the way, you'll see chemical reactions first-hand and meet entertaining characters like Enzyme Man and Drinkzilla, who show how the liver metabolizes alcohol. Together with Ami and Rin, you'll learn all about:
Whether you need a molecular biology refresher or you're just fascinated by the science of life, The Manga Guide to Molecular Biology will give you a uniquely fun and informative introduction. |