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by: R. Ian Freshney
publisher: Wiley-Blackwell, published: 2010-09-17
ASIN: 0470528125
EAN: 9780470528129
sales rank: 56282
This is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture.Since publication of the previous edition of this benchmark text, numerous types of equipment and materials have changed and new specialized techniques have been developed, particularly in the area of stem cell research and tissue engineering. These and other developments have been incorporated into this rigorously revised and expanded sixth edition of Culture of Animal Cells, the leading text in the basic methodology of cell culture. This authoritative volume offers a complete background related to growth of animal cells in culture. It contains many specialized protocols and advice on how to apply some of the basic protocols to teaching cell culture. Beginning with laboratory design, safety, validation, and bioethics, then continuing with preparation of media, primary culture, and cell lines through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the unparalleled coverage includes: An all-new section addressing the additional caution required once a primary culture is converted to a cell line Differentiation in stem cells and reprogramming of adult cells to form stem cells A new section and protocol on cryopreservation by vitrification Separate sections dealing with epithelial cells, mesenchymal cells, neurectodermal cells, and hematopoietic cells, a new chapter on germs cells/stemcells/amniocytes A new section on organotypic culture Increased emphasis on contamination and misidentification (including cross-contamination) with revised protocols for detection and elimination A problem-solving chapter, glossary of terms, advice on sourcing materials, and list of suppliers
This landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells and is indispensable for those in the field, including clinical and biopharmaceutical researchers and scientists, graduate students, laboratory managers in cell and molecular biology labs and genetics labs, trainees, and technicians.
Q&A with Author R. Ian Freshney, PhD.
 | | Author R. Ian Freshney, PhD. | R. Ian Freshney, PhD, is an honorary Senior Research Fellow in the Centre for Oncology and Applied Pharmacology at the University of Glasgow. He is a world-renowned expert on cell culture technique and has authored and edited numerous successful books, including the hugely popular Culture of Animal Cells (now in its 6th Edition), and Culture of Human Stem Cells.
Culture of Animal Cells: A Manual of Basic Technique and Specialized Application, 6th Ed has been very well received by the scientific community, as have many of your other titles. What do you think sets your books apart and makes them so successful compared to other titles in the field? I would say there are three major components: (1) The books are designed as practical guides with detailed single-step instructions which should be sufficient without further recourse to the literature. (2) The preparation and sources of materials are explained in detail. (3) Most of the basic protocols are illustrated with easy-to-follow diagrams.
What challenges did you face while putting together Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, 6th Edition? I struggled with the need to update but at the same time preserve basic procedures which have not changed markedly from the previous edition. This, of course, led to the dilemma of deciding what to retain and what to leave out. I also thought long and hard about what specialized protocols to add without compromising the basic technique role of the book.
What do you find rewarding about authoring and editing books in the sciences? It forces me to keep up-to-date with developments in cell culture, and in turn helps me to be a better teacher of cell culture. I also get satisfaction from converting what are sometimes complex instructions into one simple straightforward procedure.
What inspired you to write your first book? I was invited to prepare a textbook on cell culture for a college course. Previously, I had used John Paul’s book, Cell and Tissue Culture, and knew it needed to be updated.
What advice would you offer to scientists writing or editing their first book? - Determine the precise niche that you wish to fill. - Plan the structure carefully beforehand but do not be afraid to deviate, within reason, to meet new demands. - Ensure the text is well illustrated with tables and diagrams. - Make sure the book is properly cross-referenced. - Prepare the index yourself. Even if it is to be professionally indexed you will find terms that the indexer does not.
How would you compare the experiences of writing a book and writing a journal article? Both require a clear concise style, but the author has more freedom to express ideas and opinions when writing a book. Another major difference is that a scientific paper must address a specific topic and provide proof to substantiate the conclusions that are drawn, while a book draws on the author’s and other scientists’ experience to provide a more general review and specific guidelines or instructions.
Do you find that the role of books in the scientific research community has changed over the years? Are they valued more or less today than they were 10-20 years ago? Rapid changes in technology mean that some books will become outdated without regular updates, which are not always feasible in printed copy. Yet, while there is an increasing tendency to provide instructions and protocols online, many people will still find reassurance from using a textbook with an established reputation.
How do you envision the evolution of science writing over the next 5-10 years? Certain journals will still tend to be regarded as more reliable than others, as indicated by their citation indices. However, the number of free public access journals will probably increase, presumably with payment to submit articles. There will be a continuing need for editorial control of journal content due to the proliferation of unedited, non-reviewed material appearing online.
What do you feel has been the most significant scientific discovery that has been made during your lifetime? I can really only speak for biology where the elaboration of the genetic code is probably the most significant development. The ability to regulate gene expression and the resultant plasticity of the cell phenotype will create major opportunities in cell culture and its relevance to tissue in vivo.
What are you reading right now? This questionnaire! Otherwise, mostly novels and current journals for which I have alerts.
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publisher: Prometheus Books, published: 2006-04-04
ASIN: 1591024048
EAN: 9781591024040
sales rank: 293212
Stem cell research is headline news. Researchers are eager to move forward, state governments and private foundations are rushing to support it, and the sick and afflicted are desperate for its benefits. Yet powerful forces in our society - led by President George W. Bush - find it morally troubling and they are doing all in their powers to restrict its development beyond a very limited scale. Stem cells have the potential to develop into different parts of the body - skin, bone, nerves, blood, and more. Scientists usually harvest them from aborted foetal tissue or newly fertilised cells. This procedure has proved very unacceptable to the religious right. They argue that even a newly fertilised cell is fully human and has all of the rights of full-grown adults, and they find any use of aborted foetuses abhorrent. Given the medical potential for treatment of incurable diseases by stem cell research, as well as the moral dilemmas this technology poses, should such research be permitted? What moral, religious, or political objections might be raised? Philosophers Michael Ruse and Christopher Pynes have compiled this valuable, up-to-date, and newly revised collection of articles by noted experts to address all aspects of the stem cell controversy. The contributors - scientists, medical practitioners, philosophers, theologians, historians, and policy analysts - offer a variety of perspectives to give readers the critical tools they need to shape an informed position on the topic. Readers will come away with a deeper understanding of the science of stem cell research, its medical cures and promises, and the moral, religious, and policy concerns surrounding this controversial social issue.
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by: Joseph, Ph.D. Panno
publisher: Checkmark Books, published: 2006-10
ASIN: 081606931X
EAN: 9780816069316
sales rank: 279867
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by: Michael Williams
publisher: BloodBanker.com, published: 2011-06-13
ASIN: B0055UPCM6
sales rank: 443547
Have you ever thought a cord blood of a newborn baby can save a life? No one knew it until 1980. It was in this year when a group of scientists found a number of similarities between human cord blood and bone marrow. Three years later, an important confirmation was made and it's suggested cord blood stem cells were good alternative for bone marrow in transplantation. Hundreds of people in the world who are diagnosed with deadly diseases such as leukemia and brain cancer were given a chance to live normally and perform their everyday routine without feeling pain. A cord blood transplant save their lives. However, it's still inevitable some groups create contrasting issues about this miracle cure. They have questions and this book provides you the answers!
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by: Christine Mummery
publisher: Academic Press, published: 2011-01-14
ASIN: 0123815355
EAN: 9780123815354
sales rank: 882200
In the past decades our understanding of stem cell biology has increased tremendously. Many types of stem cells have been discovered in tissues of which everyone presumed were unable to regenerate in adults; these include particularly the heart and the brain. There is vast interest in stem cells from biologists and clinicians who see the potential for regenerative medicine and future treatments for chronic diseases like Parkinson, diabetes and spinal cord lesions based on the use of stem cells and entrepreneurs in biotechnology who expect new commercial applications ranging from drug discovery to transplantation therapies. As is often the case in science, many early claims turned out to be different from those expected. Embryonic stem cell therapies have not moved rapidly into clinical practice. Adult stem cells certainly have given certain degrees of success but not nearly to the extent that advocates would have wished for. Some claims of early successes in adult stem cell therapies have not been sustained in double-blinded, randomized clinical trials. Some claims are now close to routine therapy. Some of the claims not supported by evidence have nevertheless reached private clinical practice so that "stem cell tourism" is beginning to reach exaggerated proportions. This book provides the reader background information on stem cells in a clear and well-organized manner. It provides the non-stem cell expert with an understandable review of the history, current state of affairs, and facts and fiction of the promises of stem cells. It distinguishes itself from the multiplicity of websites on the subject of stem cells by being scientifically, politically and ethically neutral, explaining pros and cons for stem cells of every sort with the intention of reaching a wide readership ranging from advanced students and patient advocacy groups to clinicians, specialists and early phase medics in training. By providing the background scientific and social information, it provides readers with the information they require to form their own opinions on the use of stem cells on the basis of facts rather than hype. * Explains in straightforward, non-specialist language the basic biology of stem cells and their applications in modern medicine and future therapy * Includes extensive coverage of adult and embryonic stem cells both historically and in contemporary practice * Richly illustrated to assist in understanding how research is done and the current hurdles to clinical practice
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by: Robin Craig
publisher: ThoughtWare Australia Pty Ltd, published: 2012-04-05
ASIN: B007RVLLQ2
sales rank: 509972
The ethical questions surrounding human cloning and stem cell research cannot be answered without deciding what rights a human embryo has, and that cannot be decided without first deciding what human rights are and where they come from.
In this philosophical essay (updated from controversial but popular public talks to university student, professional and community groups in 2002-2003), these questions are approached and answered in the context of a secular, objective theory of ethics and rights. Because of the nature of rights, embryos have no rights: and legal enforcement of such "rights" actually violates human rights.
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by: Jennifer G. Treleaven MD FRCP FRCPath
publisher: Churchill Livingstone, published: 2008-10-09
ASIN: 0443101477
EAN: 9780443101472
sales rank: 771101
A guide to the practice of stem cell transplantation, its status in the treatment of various disorders and the problems that arise after transplantation, aimed at the whole transplant team.- An up to date guide to best practice in the use of stem cell transplantation, covering current status in the treatment of malignant and non-malignant conditions, practical aspects and problems such as infection and graft versus host disease.
- Has a practical, accessible approach with free use of algorithms, list tables.
- Aimed at the whole transplant team - this is an interdisciplinary field.
- International contributor team with editors in the UK and USA.
- Illustrated in colour throughout.
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publisher: University of California Press, published: 2007-12-11
ASIN: 0520252128
EAN: 9780520252127
sales rank: 980124
Few recent advances in science have generated as much excitement and controversy as human embryonic stem cells. The potential of these cells to replace diseased or damaged cells in virtually every tissue of the body heralds the advent of an extraordinary new field of medicine. Controversy arises, however, because current techniques required to harvest stem cells involve the destruction of the human blastocyst. This even-handed, lucidly written volume is an essential tool for understanding the complex issues--scientific, religious, ethical, and political--that currently fuel public debate about stem cell research. One of the few books to provide a comprehensive overview for a wide audience, the volume brings together leading scientists, ethicists, political scientists, and doctors to explain this new scientific development and explore its ramifications.
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by: Susan Ezzone
publisher: Jones & Bartlett Publishers, published: 2006-05-22
ASIN: 076374719X
EAN: 9780763747190
sales rank: 932800
Blood and Marrow Stem Cell Transplantation, Third Edition serves as a convenient reference for oncology nurses caring for blood and marrow stem cell transplantation recipients. The Third Edition has been updated and revised to reflect the most current research related to the past, present, and future of blood and marrow stem cell transplants. This resource covers everything from preparing for transplantation, to recovery-related issues and delayed outcomes. Basic background information on transplantation procedures and processes are provided, along with in-depth coverage of pulmonary, cardiac, renal and hepatic, neurologic, and hematologic effects. A chapter devoted to controversial ethical issues is also provided. Blood and Marrow Stem Cell Transplantation, Third Edition presents complete, up-to-date coverage of the transplantation process from beginning to end!
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by: Dr William Rader
publisher: Nanog Publishing Inc., published: 2010-03-01
ASIN: 0615329055
EAN: 9780615329055
sales rank: 267050
If you thought the time of dramatic evolutionary advancement in medical knowledge was over, brace yourself for the untold story of a doctor who singlehandedly, in the face of disdain from his colleagues and threats from government bureaucrats, made a breakthrough scientific discovery. Dr. Rader discovered how to apply fetal stem cell technology to humans and has eased the suffering and at times saved the lives of more than 1,500 patients with Autism, Epilepsy, Multiple Sclerosis, Stroke, Parkinson's disease, Muscular Dystrophy, and many other diseases since 1995.Dr. Rader, familiar to millions as a respected medical expert on ABC-TV, reveals the astounding truth about the most extensive fetal stem cell trials ever conducted on human patients - mostly so - called "hopeless" cases. Blocked in the USA reveals the science behind Dr. Rader's breakthroughs, the struggles with the medical establishment, and, most important, the detailed successful results of his work. Now the truth can be told, and it reads like an adventure story, spanning three continents as one doctor dared to defy the FDA, the medical establishment and the drug companies.
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