transgenic books

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Transgenic Maize: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2009-03-05
ASIN: 1934115495
EAN: 9781934115497
sales rank: 1680260
As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs.

Transgenic Crop Plants: Volume 1: Principles and Development

publisher: Springer, published: 2010-02-03
ASIN: 3642048080
EAN: 9783642048081
sales rank: 3548790
Development of transgenic crop plants, their utilization for improved agriculture, health, ecology and environment and their socio-political impacts are currently important fields in education, research and industries and also of interest to policy makers, social activists and regulatory and funding agencies. This work prepared with a class-room approach on this multidisciplinary subject will fill an existing gap and meet the requirements of such a broad section of readers. Volume 1 with ten chapters contributed by 31 eminent scientists from nine countries deliberates on the basic concepts, strategies and tools for development of transgenic crop plants, including topics such as: explants used for the generation of transgenic plants, gene transfer methods, organelle transformation, selection and screening strategies, expression and stability of transgenes, silencing undesirable genes, transgene integration, biosynthesis and biotransformation and metabolic engineering of pathways and gene discovery.

Values and Objectivity in Science: The Current Controversy about Transgenic Crops

by: Hugh Lacey
publisher: Lexington Books, published: 2005-08
ASIN: 0739111418
EAN: 9780739111413
sales rank: 2212427
Values and Objectivity in Science illuminates many of the ethical issues that arise concerning scientific practices and applications, offering an account of how social and ethical values play important roles within science. Hugh Lacey develops and clarifies his previous analysis by arguing for the importance of research being conducted under a plurality of strategies, contrasting 'materialist strategies' with 'agro-ecological strategies.' By displaying the structure of current ethical controversies about the legitimacy of planting transgenic crops, this book illustrates that sound thinking on such issues must be grounded on an adequate philosophy of science, one that can clearly distinguish between the proper and the distorting roles of values in scientific practices. This book will prove useful for science students and practitioners as well as those interested in the growing ethical questions involved in scientific practices.

Transgenic and Mutant Tools to Model Brain Disorders (Neuromethods)

publisher: Humana Press, published: 2010-01-12
ASIN: 1607614731
EAN: 9781607614739
sales rank: 2920806
As the presence of genetically modified animal models in research laboratories has multiplied, the role of genetic factors in the pathogenesis of brain disorders has become particularly important. The refinement of molecular genetic methods has continued to broaden our understanding of the genetic factors associated with a variety of disorders. In "Transgenic and Mutant Tools to Model Brain Disorders", leading scientists specializing in this field contribute a timely collection of recent advances featuring a vast array of topics in order to contribute to the diverse approaches taken toward the evaluation of genetically modified models in biomedical research. Opening with several chapters covering general aspects of genetically modified animal models, the book then continues with detailed chapters on models of specific human brain disorders, including OCD, Rett Syndrome, anxiety disorders, depression, and schizophrenia. As a volume in the successful Neuromethods™ series, the chapters provide authoritative reviews covering the most commonly used approaches in the field. Cutting-edge and concise, "Transgenic and Mutant Tools to Model Brain Disorders" offers a comprehensive and descriptive overview on a variety of topics in neuroscience and biological psychiatry.

Genetic Glass Ceilings: Transgenics for Crop Biodiversity

by: Jonathan Gressel
publisher: The Johns Hopkins University Press, published: 2008-02-12
ASIN: 0801887194
EAN: 9780801887192
sales rank: 2272779

As the world’s population rises to an expected ten billion in the next few generations, the challenges of feeding humanity and maintaining an ecological balance will dramatically increase. Today we rely on just four crops for 80 percent of all consumed calories: wheat, rice, corn, and soybeans. Indeed, reliance on these four crops may also mean we are one global plant disease outbreak away from major famine.
In this revolutionary and controversial book, Jonathan Gressel argues that alternative plant crops lack the genetic diversity necessary for wider domestication and that even the Big Four have reached a "genetic glass ceiling": no matter how much they are bred, there is simply not enough genetic diversity available to significantly improve their agricultural value. Gressel points the way through the glass ceiling by advocating transgenics—a technique where genes from one species are transferred to another. He maintains that with simple safeguards the technique is a safe solution to the genetic glass ceiling conundrum. Analyzing alternative crops—including palm oil, papaya, buckwheat, tef, and sorghum—Gressel demonstrates how gene manipulation could enhance their potential for widespread domestication and reduce our dependency on the Big Four. He also describes a number of ecological benefits that could be derived with the aid of transgenics.
A compelling synthesis of ideas from agronomy, medicine, breeding, physiology, population genetics, molecular biology, and biotechnology, Genetic Glass Ceilings presents transgenics as an inevitable and desperately necessary approach to securing and diversifying the world's food supply.
(2008)

Transgenic Microalgae as Green Cell Factories (Advances in Experimental Medicine and Biology)

publisher: Springer, published: 2007-11-26
ASIN: 0387755314
EAN: 9780387755311
sales rank: 2583080

Microalgae have been largely commercialized as food and feed additives, and their potential as a source of high-added value compounds is well known. Yet, only a few species of microalgae have been genetically transformed with efficiency. A better understanding of the mechanisms that control the regulation of gene expression in eukaryotes is therefore needed. In this book a group of outstanding researchers working on different areas of microalgae biotechnology offer a global vision of the genetic manipulation of microalgae and their applications.

Transgenic Animal Technology, Second Edition: A Laboratory Handbook

by: Carl A. Pinkert
publisher: Academic Press, published: 2002-12-02
ASIN: 0125571666
EAN: 9780125571661
sales rank: 2464630
Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal considerations - for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems.

Contains a comprehensive collection of transgenic animal and gene transfer methods
Discusses background and introduction to techniques and animal systems
Teaches practical step-by-step protocols
New section on analysis

Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in Molecular Biology)

publisher: Humana Press, published: 1998-02-24
ASIN: 0896033856
EAN: 9780896033856
sales rank: 2764449
A comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus.

Transgenic and Knockout Models of Neuropsychiatric Disorders (Contemporary Clinical Neuroscience)

publisher: Humana Press, published: 2010-11-09
ASIN: 1617376078
EAN: 9781617376078
sales rank: 4248569
In this up-to-date survey and critical assessment of transgenic and knockout models in neuropsychiatry and behavior, a panel of leading researchers comprehensively assesses how and whether the genetic abnormalities produced from these models manifest the neuropsychiatric disorders to which they correspond. The authors focus on transgenic and knockout models of neurocognitive dysfunction and neuropsychiatric dysfunction. The discussion of neurobiological problems covers mental retardation, polyglutamate, and speech disorders, as well as disorders that involve cognitive, social, speech, and language dysfunction. The neuropsychiatric dysfunctions examined include psychosis and schizophrenia, anxiety, depression, and bipolar disorder.

Transgenic Animals

by: Louis-Marie Houdebine
publisher: CRC Press, published: 1997-05-24
ASIN: 9057020696
EAN: 9789057020698
sales rank: 4627466
During the past 20 years, transgenesis has become a popular technique and a crucial tool for molecular geneticists and biologists. Transgene expression is now better-controlled and even specifically inducible by exogenous factors. While these techniques have quite significantly transformed the experimental approaches taken by biologists, the applications are more limited than expected and concerns have arisen regarding biosafety as well as physiological, social, and philosophical issues.

Transgenic Animals: Generation and Use contains articles on the techniques used to generate transgenic animals and a section on the preparation of vectors for the optimally controlled expression of transgenes. It also examines the use of transgenic animals in the study of gene function and human diseases, the preparation of recombinant proteins and organs for pharmaceutical and medical use, and the improvement of genetic characteristics of farm animals. Finally, it discusses more recent problems generated by transgenic animals including conservation of transgenic lines, specific database patenting, biosafety, and bioethics.

Drawn from both academia and industry, the contributors to this monograph present in one concise volume all the relevant information on the different aspects of transgenesis. This book can be used as both a reference book and a textbook for specialized university courses and will be of interest to everyone involved in basic research in animal biology, molecular genetics, animal biotechnology, pharmaceutical science, and medicine.

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	Transgenic Maize: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2009-03-05 ASIN: 1934115495 EAN: 9781934115497 sales rank: 1680260 As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs.
	Transgenic Crop Plants: Volume 1: Principles and Development publisher: Springer, published: 2010-02-03 ASIN: 3642048080 EAN: 9783642048081 sales rank: 3548790 Development of transgenic crop plants, their utilization for improved agriculture, health, ecology and environment and their socio-political impacts are currently important fields in education, research and industries and also of interest to policy makers, social activists and regulatory and funding agencies. This work prepared with a class-room approach on this multidisciplinary subject will fill an existing gap and meet the requirements of such a broad section of readers. Volume 1 with ten chapters contributed by 31 eminent scientists from nine countries deliberates on the basic concepts, strategies and tools for development of transgenic crop plants, including topics such as: explants used for the generation of transgenic plants, gene transfer methods, organelle transformation, selection and screening strategies, expression and stability of transgenes, silencing undesirable genes, transgene integration, biosynthesis and biotransformation and metabolic engineering of pathways and gene discovery.
	Values and Objectivity in Science: The Current Controversy about Transgenic Crops by: Hugh Lacey publisher: Lexington Books, published: 2005-08 ASIN: 0739111418 EAN: 9780739111413 sales rank: 2212427 Values and Objectivity in Science illuminates many of the ethical issues that arise concerning scientific practices and applications, offering an account of how social and ethical values play important roles within science. Hugh Lacey develops and clarifies his previous analysis by arguing for the importance of research being conducted under a plurality of strategies, contrasting 'materialist strategies' with 'agro-ecological strategies.' By displaying the structure of current ethical controversies about the legitimacy of planting transgenic crops, this book illustrates that sound thinking on such issues must be grounded on an adequate philosophy of science, one that can clearly distinguish between the proper and the distorting roles of values in scientific practices. This book will prove useful for science students and practitioners as well as those interested in the growing ethical questions involved in scientific practices.
	Transgenic and Mutant Tools to Model Brain Disorders (Neuromethods) publisher: Humana Press, published: 2010-01-12 ASIN: 1607614731 EAN: 9781607614739 sales rank: 2920806 As the presence of genetically modified animal models in research laboratories has multiplied, the role of genetic factors in the pathogenesis of brain disorders has become particularly important. The refinement of molecular genetic methods has continued to broaden our understanding of the genetic factors associated with a variety of disorders. In "Transgenic and Mutant Tools to Model Brain Disorders", leading scientists specializing in this field contribute a timely collection of recent advances featuring a vast array of topics in order to contribute to the diverse approaches taken toward the evaluation of genetically modified models in biomedical research. Opening with several chapters covering general aspects of genetically modified animal models, the book then continues with detailed chapters on models of specific human brain disorders, including OCD, Rett Syndrome, anxiety disorders, depression, and schizophrenia. As a volume in the successful Neuromethods™ series, the chapters provide authoritative reviews covering the most commonly used approaches in the field. Cutting-edge and concise, "Transgenic and Mutant Tools to Model Brain Disorders" offers a comprehensive and descriptive overview on a variety of topics in neuroscience and biological psychiatry.
	Genetic Glass Ceilings: Transgenics for Crop Biodiversity by: Jonathan Gressel publisher: The Johns Hopkins University Press, published: 2008-02-12 ASIN: 0801887194 EAN: 9780801887192 sales rank: 2272779 As the world’s population rises to an expected ten billion in the next few generations, the challenges of feeding humanity and maintaining an ecological balance will dramatically increase. Today we rely on just four crops for 80 percent of all consumed calories: wheat, rice, corn, and soybeans. Indeed, reliance on these four crops may also mean we are one global plant disease outbreak away from major famine. In this revolutionary and controversial book, Jonathan Gressel argues that alternative plant crops lack the genetic diversity necessary for wider domestication and that even the Big Four have reached a "genetic glass ceiling": no matter how much they are bred, there is simply not enough genetic diversity available to significantly improve their agricultural value. Gressel points the way through the glass ceiling by advocating transgenics—a technique where genes from one species are transferred to another. He maintains that with simple safeguards the technique is a safe solution to the genetic glass ceiling conundrum. Analyzing alternative crops—including palm oil, papaya, buckwheat, tef, and sorghum—Gressel demonstrates how gene manipulation could enhance their potential for widespread domestication and reduce our dependency on the Big Four. He also describes a number of ecological benefits that could be derived with the aid of transgenics. A compelling synthesis of ideas from agronomy, medicine, breeding, physiology, population genetics, molecular biology, and biotechnology, Genetic Glass Ceilings presents transgenics as an inevitable and desperately necessary approach to securing and diversifying the world's food supply. (2008)
	Transgenic Microalgae as Green Cell Factories (Advances in Experimental Medicine and Biology) publisher: Springer, published: 2007-11-26 ASIN: 0387755314 EAN: 9780387755311 sales rank: 2583080 Microalgae have been largely commercialized as food and feed additives, and their potential as a source of high-added value compounds is well known. Yet, only a few species of microalgae have been genetically transformed with efficiency. A better understanding of the mechanisms that control the regulation of gene expression in eukaryotes is therefore needed. In this book a group of outstanding researchers working on different areas of microalgae biotechnology offer a global vision of the genetic manipulation of microalgae and their applications.
	Transgenic Animal Technology, Second Edition: A Laboratory Handbook by: Carl A. Pinkert publisher: Academic Press, published: 2002-12-02 ASIN: 0125571666 EAN: 9780125571661 sales rank: 2464630 Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal considerations - for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols New section on analysis
	Plant Virology Protocols: From Virus Isolation to Transgenic Resistance (Methods in Molecular Biology) publisher: Humana Press, published: 1998-02-24 ASIN: 0896033856 EAN: 9780896033856 sales rank: 2764449 A comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus.
	Transgenic and Knockout Models of Neuropsychiatric Disorders (Contemporary Clinical Neuroscience) publisher: Humana Press, published: 2010-11-09 ASIN: 1617376078 EAN: 9781617376078 sales rank: 4248569 In this up-to-date survey and critical assessment of transgenic and knockout models in neuropsychiatry and behavior, a panel of leading researchers comprehensively assesses how and whether the genetic abnormalities produced from these models manifest the neuropsychiatric disorders to which they correspond. The authors focus on transgenic and knockout models of neurocognitive dysfunction and neuropsychiatric dysfunction. The discussion of neurobiological problems covers mental retardation, polyglutamate, and speech disorders, as well as disorders that involve cognitive, social, speech, and language dysfunction. The neuropsychiatric dysfunctions examined include psychosis and schizophrenia, anxiety, depression, and bipolar disorder.
	Transgenic Animals by: Louis-Marie Houdebine publisher: CRC Press, published: 1997-05-24 ASIN: 9057020696 EAN: 9789057020698 sales rank: 4627466 During the past 20 years, transgenesis has become a popular technique and a crucial tool for molecular geneticists and biologists. Transgene expression is now better-controlled and even specifically inducible by exogenous factors. While these techniques have quite significantly transformed the experimental approaches taken by biologists, the applications are more limited than expected and concerns have arisen regarding biosafety as well as physiological, social, and philosophical issues. Transgenic Animals: Generation and Use contains articles on the techniques used to generate transgenic animals and a section on the preparation of vectors for the optimally controlled expression of transgenes. It also examines the use of transgenic animals in the study of gene function and human diseases, the preparation of recombinant proteins and organs for pharmaceutical and medical use, and the improvement of genetic characteristics of farm animals. Finally, it discusses more recent problems generated by transgenic animals including conservation of transgenic lines, specific database patenting, biosafety, and bioethics. Drawn from both academia and industry, the contributors to this monograph present in one concise volume all the relevant information on the different aspects of transgenesis. This book can be used as both a reference book and a textbook for specialized university courses and will be of interest to everyone involved in basic research in animal biology, molecular genetics, animal biotechnology, pharmaceutical science, and medicine.