Related books on 'flow-cytometry'
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publisher: Humana Press, published: 2010-12-01 ASIN: 1617379492 EAN: 9781617379499 sales rank: 2013796 With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users. |
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publisher: Springer, published: 2011-11-02 ASIN: 9400724179 EAN: 9789400724174 sales rank: 2723653 Introduction of new technologies and their applications to neuroblastoma diagnosis, treatment, and therapy assessment are explained. Role of molecular ghenetics in diagnosis and therapy for neuroblastoma patients is detailed. Molecular detection of minimal residual neuroblastoma is described. Magnetic resonance imaging and spectroscopy are detailed for diagnosing this solid, extracranial cancer. Targets for therapeutic intervention in neuroblastoma are identified, including targeting multidrug resistance in this cancer. Ornithine decarboxylase and polyamines are novel targets for therapeutic intervention. The effectiveness of chemotherapy with oral irinotecan and temozolomide is explained. The role of transcription factors (GATA) in neuroblastoma pregression is also included. |
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publisher: Academic Press, published: 1994-11-21 ASIN: 0125641427 EAN: 9780125641425 sales rank: 1444942 Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells. Key Features * Completely revised and greatly expanded since the publication of the First Edition in 1990 * Methods cover cell death and cell cycle analyses Practical, handbook-style presentation works in lab or classroom * Unique comprehensive methodological coverage * Color plates illustrate techniques * In-depth treatment of procedures, including a description of each procedure: * Theoretical foundations * Critical aspects * Possible pitfalls * Written by authors with extensive experience who: * Developed or modified the techniques * Describe their experience with different instruments and applications to different cell systems * Are the Who's Who in Flow Cytometry |
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publisher: Wiley-Blackwell, published: 2010-08-09 ASIN: 0470543981 EAN: 9780470543986 sales rank: 1545573 A much-needed primer on the use of laser flow cytometry for stem cell analysis Laser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation, and apoptosis. However, no resources specifically address the use of this methodology for the study of stem cells; this is especially important as stem cell analysis involves specialized methods and staining procedures based on specific characteristics such as marker expression, cell size, drug transport, and efflux of the stem cells. Now, this book reviews these procedures, discusses the science behind them, and provides real-world examples to illustrate the usefulness of the methods. It brings together world-class experts in pathology, biophysics, immunology, and stem cell research, who draw upon their extensive experience with the methods and show examples of good data to help guide researchers in the right direction. Chapter coverage includes:
This book is an essential resource for all graduate students, practitioners in developing countries, libraries and book repositories of universities and research institutions, and individual researchers. It is also of interest to laboratories engaged in stem cell research and use of stem cells for tissue regeneration, and to any organization dealing in stem cell and tissue regeneration research. |
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by: Ulrich Sack publisher: S. Karger Publishing, published: 2008-11-17 ASIN: 3805585551 EAN: 9783805585552 sales rank: 2183636 This book is the updated English version of the 2006 German bestseller "Zellulare Diagnostik", a comprehensive presentation of flow cytometry and its applications. While some techniques of immunophenotyping by flow cytometry already are routine procedures in the laboratory, new methods for the functional characterization of cells, the analysis of rare cells, and the diagnosis of complex materials have only begun to win wide recognition. New approaches such as slide-based cytometry will lead to an increase in the use of cytometric techniques. Multiparameter approaches will further improve analysis. The book provides a comprehensive and detailed compilation of all aspects of flow cytometry in research and the clinic. For newcomers it offers a thorough introduction, for advanced users, specific protocols and interpretation assistance. |
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publisher: Springer, published: 2011-12-02 ASIN: 1461411734 EAN: 9781461411734 sales rank: 4356830 Pediatric oncology is a considerably complex discipline requiring frequent need for reference material. Emergencies in Pediatric Oncology meets this objective by serving as a concise, practical resource for the hurried physician. Replete with diagrams and written in a clear, succinct style, this title offers an easy-to-read guide especially useful for residents, fellows, and nurses. Providing an overview of the epidemiology of childhood cancer and the history of its treatment, this title also addresses a range of key problems in the pediatric oncologic setting, covering the most common emergencies and providing short, practical guidelines on how to handle them. Written by authorities from an array of disciplines, this helpful resource provides answers and concrete guidance and is a valuable contribution to the literature in pediatric oncology. |
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publisher: Wiley, published: 2011-01-11 ASIN: 0470433566 EAN: 9780470433560 sales rank: 2131584 This book covers the unique application of flow cytometry in drug discovery and development. The first section includes two introductory chapters, one on flow cytometry and one on biomarkers, as well as a chapter on recent advances in flow cytometry. The second section focuses on the unique challenges and added benefits associated with the use of flow cytometry in the drug development process. The third section contains a single chapter presenting an in depth discussion of validation considerations and regulatory compliance issues associated with drug development. |
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by: Marilyn A. Owens publisher: Wiley-Liss, published: 1994-12-20 ASIN: 0471021768 EAN: 9780471021766 sales rank: 2259788 FLOW CYTOMETRY PRINCIPLES FOR CLINICAL LABORATORY PRACTICE Quality Assurance for Quantitative Immunophenotyping Marilyn A. Owens and Michael R. Loken Flow Cytometry Principles for Clinical Laboratory Practice is an invaluable benchtop reference and introduction for first-time users of flow cytometry in clinical diagnostic laboratories. This manual provides authoritative coverage of accepted methods, quality controls, and standards for the cytometric analysis of clinical samples with particular relevance to CD4+ lymphocytes. CD4+ lymphocyte determination by immunophenotyping is the standard method for evaluating the status of HIV infection, and the single most frequent application of flow cytometry in a diagnostic setting. Flow Cytometry Principles for Clinical Laboratory Practice will appeal to clinical laboratory technologists, medical practitioners, and cytometry lab directors in their role as instructors of new personnel. In addition, it is a valuable resource for clinical and biomedical researchers, establishing quantitative cytometric systems for studies in immunology and hematology. |
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publisher: Academic Press, published: 1992-02-27 ASIN: 0124329403 EAN: 9780124329409 sales rank: 9003291 This book reviews flow cytometric methods (techniques for measuring and sorting of cells) used in hematology--ranging from those in routine use (such as leukocyte counting and immunophentotyping in diseases like leukemia and AIDS) to those that have potential future use in experimental and clinical hematology. This volume will be of interest to a wide audience, including cell biologists, hematologists, cancer researchers, and HIV/AIDS researchers. |
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by: Tsieh Sun MD publisher: Lippincott Williams & Wilkins, published: 2007-10-29 ASIN: 078178400X EAN: 9780781784009 sales rank: 1534165 This text is a detailed guide to the use of flow cytometry, immunohistochemistry, and molecular genetic techniques for diagnosis of hematologic neoplasms. Dr. Sun explains the principles of these techniques and demonstrates their utility in 39 clinical cases covering all important entities. Each case represents a comprehensive diagnostic approach including a clinical history and flow cytometric, immunohistochemical, and molecular genetic findings. Abundant full-color illustrations show histologic sections, immunohistochemical stains, bone marrow, peripheral blood, and body fluid smears, and each case includes a complete set of flow cytometric histograms. Over 100 tables compare and differentiate the diagnostic features of similar diseases. An image bank will be available on a companion Website. |