Related books on 'methods-in-molecular-biology'
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by: Lauren Pecorino publisher: Oxford University Press, USA, published: 2008-05-06 ASIN: 0199211485 EAN: 9780199211487 sales rank: 196327 Many of the cells in our body undergo tightly regulated replication to enable growth to occur and to ensure that damaged or worn out tissues are replaced. Regulation is mediated via many different mechanisms. It is when these mechanisms fail, and regulation is lost, that cancer can result. Recent advances in molecular cell biology have greatly expanded our understanding of the processes and mechanisms which underpin the regulation of cell replication, and what is happening at the molecular level when these fail. Now in its second edition, The Molecular Biology of Cancer: Mechanisms, Targets, and Therapeutics gives a fresh approach to the study of the molecular basis of cancer. Written with the undergraduate student in mind, Lauren Pecorino focuses on how our understanding of the defective mechanisms which drive cancer is leading to the development of new targeted therapeutic agents. Opening with an overview of the key hallmarks of cancer as a disease state, the book then leads us through the principal components of the regulatory machinery through which cell division is controlled and corrupted with the onset of cancer. Within each chapter, students are shown how this molecular knowledge is being applied to develop new targeted therapeutic strategies. Ideal for courses in the molecular biology of cancer and cancer biology, The Molecular Biology of Cancer, Second Edition, is an invaluable resource for any student wishing to gain insight into the molecular basis of the disease and formulate possible solutions for its effective control. New to this Edition · Updated research · Three brand new chapters: - "The Cell Cycle": Gives extended treatment of this central aspect of cancer biology - "Infections and Inflammation": Focuses on the relationship between infectious agents and inflammation and the onset of cancer - "The Cancer Industry": Provides an overview of the process through which drugs are developed and tested · Enhanced coverage of apoptosis · Over 25 new figures plus a new color plate section · A new "How do we know that?" feature, in which experimental methods and results are discussed, thus shedding light on how our understanding of cancer biology is developed · An extended glossary · A companion website featuring additional resources for both instructors and students: - For instructors, the site offers figures from the book available to download and a testbank of multiple-choice questions with feedback linked to the book for use in formative or summative assessment - For students, the website features a web link library as well as hyperlinks to primary literature articles cited in the text |
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by: Larry Snyder publisher: ASM Press, published: 2007-05-31 ASIN: 1555813992 EAN: 9781555813994 sales rank: 49297 This landmark volume provides the single most comprehensive and authoritative textbook on bacterial molecular genetics. Perfect for advanced undergraduate and graduate-level courses, the text presents the latest research on the subject in a clearly written and well-illustrated style. It provides descriptive background information, detailed experimental methods, examples of genetic analyses, and advanced material relevant to current applications of molecular genetics. While providing a deep understanding of bacterial molecular genetics, the material is integrated with biochemical, genomic, and structural information to broaden understanding. The approach centers on the most-studied bacteria, Escherichia coli and Bacillus subtilis. In addition, examples from other bacteria with medical, ecological, or biotechnological significance are covered throughout the text. The material in each chapter has been substantially revised and rewritten and reflects exciting developments in the field of bacterial molecular genetics and its relationship to other fields, including genetics, biotechnology, and bioengineering. Molecular Genetics of Bacteria, 3rd Edition is organized like the two previous editions. Each chapter contains a summary of main points, descriptions of significant experiments, a set of discussion questions and a problem set (with answers to both at the back of the book), and a list of suggested readings-all updated to reflect the most recent advances in the field. Additionally, text boxes present intriguing information on each topic without interrupting the continuity of the text. This third edition is invaluable to anyone working in the fields of microbiology, genetics, biochemistry, bioengineering, medicine, molecular biology, and biotechnology. It is also essential reading for scientists in all fields of biology, many of whom depend upon the concepts and techniques covered in this book. New in the Third Edition* Features completely revised and updated material, incorporating the most recent advances in the field since publication of the second edition in 2002* Introduces over 100 brand-new illustrations* Offers a revised and expanded glossary containing approximately 1,000 entries Key Features and Benefits* Each chapter includes a concise end-of-chapter summary of the material covered* Incorporates text boxes that introduce readers to exciting developments and related topics of interest* Provides thought questions, problems, and suggested reading lists at the conclusion of each chapter that test student comprehension and encourage further research* Written by only two authors, both of whom are specialists in the field and who have significant experience teaching the subject of bacterial molecular genetics* Integrates biochemical, genomic, and structural information that broadens the knowledge obtained from molecular genetics* Focuses on the most widely studied bacteria, Escherichia coli and Bacillus subtilis, but incorporates many examples from other bacteria of medical, ecological, and biotechnological importance |
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publisher: Humana Press, published: 2011-09-29 ASIN: 1617792276 EAN: 9781617792274 sales rank: 339257 We are entering a particularly fruitful period in evolutionary genetics, as rapid technological progress transforms the investigation of genetic variation within and between species. Molecular Methods for Evolutionary Genetics is a collection of advanced molecular biology protocols and general overviews intended to represent the essential methods currently bringing evolutionary genetics to fruition. Divided into six thematic sections, this volume covers methods for characterizing genomes, diverse approaches to enrich DNA for subsets of the genome prior to sequencing, and state-of-the-art protocols for sampling genetic variation for genetic mapping studies and population genetic studies (RAD sequencing, Sequenom, microarrays, etc.). The volume concludes by focusing on methods to study candidate genes, from obtaining their sequences and analyzing their transcripts to experimentally manipulating their activities in vivo. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Molecular Methods for Evolutionary Genetics serves as a rich resource to biologists interested in evolution, whether they be specialists or beginners in molecular biology. |
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publisher: CRC Press, published: 2011-12-12 ASIN: 1420069381 EAN: 9781420069389 sales rank: 907913 Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology. Now in its third edition, this volume provides researchers with an updated tool kit that incorporates conventional as well as modern approaches to tackle biological and medicinal research in the post-genomics era. Significantly revised to address these recent changes, the editors have evaluated, revised, and sometimes replaced protocols with more efficient, more reliable, or simpler ones. The book has also been reorganized with section headings focusing on different biological levels connected to one another, taking into account the central dogma of biology (DNA → RNA → protein → metabolites). The book first explores traditional approaches and then moves to the modern "omics" approaches, including genomics, proteomics, and metabolomics. It also discusses the manipulation of biological systems (including RNAi) and macromolecular analyses, focusing on the use of microscopy. In each chapter, various notes and cautionary considerations are presented for potentially hazardous reagents. Filled with diagrams, tables, and figures to clarify methods, most chapters also contain Troubleshooting Guides indicating problems, possible causes, and solutions that may be incurred in carrying out the procedures. Researchers and scientists who master the techniques in this book are putting themselves at the cutting edge of biological and medicinal research. |
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publisher: Humana Press, published: 2010-11-02 ASIN: 1607619830 EAN: 9781607619833 sales rank: 463328 Developed for a range of tissues where the culture environment takes into account the spatial organization of the cells therein, 3D cell culture models serve to bridge the gap between in vivo studies at one extreme with that of simple cell monolayers at the other. In 3D Cell Culture: Methods and Protocols, international experts describe a number of basic and applied methodologies taken from a breadth of scientific and engineering disciplines, many of which deal with direct applications of 3D culture models, most notably in the formation of tissues for clinical purpose. Beginning with an overview of the biological and materials scaffold requirements for successfully creating 3D models, the book delves into topics such as general scaffold design and fabrication techniques, models for bone, skin, cartilage, nerve, bladder, and hair follicles, and chapters on bioreactor design, imaging, and stem cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, 3D Cell Culture: Methods and Protocols serves as a basic manual for laboratory-based scientists who not only need to have a comprehensive range of techniques contained within a single text but also require techniques described using a standard, convenient format. |
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publisher: Academic Press, published: 1991-02-11 ASIN: 0123106702 EAN: 9780123106704 sales rank: 2320045 Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Basic Methods in Yeast Genetics**Physical and genetic mapping**Making and recovering mutants**Cloning and Recombinant DNA Methods**High-efficiency transformation**Preparation of yeast artificial chromosome vectors**Basic Methods of Cell Biology**Immunomicroscopy**Protein targeting assays**Biochemistry of Gene Expression**Vectors for regulated expression**Isolation of labeled and unlabeled DNA, RNA, and protein |
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publisher: Humana Press, published: 2011-03-22 ASIN: 1617790885 EAN: 9781617790881 sales rank: 310323 Due to their novel concepts and extraordinary high-throughput sequencing capacity, the “next generation sequencing” methods allow scientists to grasp system-wide landscapes of the complex molecular events taking place in various biological systems, including microorganisms and microbial communities. These methods are now being recognized as essential tools for a more comprehensive and deeper understanding of the mechanisms underlying many biological processes. In High-Throughput Next Generation Sequencing: Methods and Applications, experts in the field explore the most recent advances in the applications of next generation sequencing technologies with an emphasis on microorganisms and their communities; however, the methods described in this book will also offer general applications relevant to the study of any living organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, High-Throughput Next Generation Sequencing: Methods and Applications is an excellent collection of chapters to aid all scientists who wish to apply these innovative research tools to enhance their own pursuits in microbiology and also biology in general. |
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by: John R. Crowther publisher: Humana Press, published: 2010-11-19 ASIN: 1617378844 EAN: 9781617378843 sales rank: 1182003 The Enzyme Linked Immunosorbent Assay (ELISA) continues, as predicted, to be the predominant assay used in biomedical and agricultural research. While little has changed in the principles of the ELISAs used since the publication of The ELISA Guidebook, many technological advantages, including the increasing number of reagents available for exploitation and the larger spectrum of instruments to measure the coloured products of ELISAs, have greatly benefited the field. Other testing technologies using molecular techniques, predominantly the conventional and more and more often, real time PCR, are rapidly evolving to help diagnose and differentiate disease agents and identify substances present in exquisitely small amounts. The ELISA is still needed either alone in mass screening, such as serological assays, or used in tandem with molecular techniques to fully evaluate the molecular and antigenic nature of analytes. John R. Crowther provides today’s premier practical guide to the understanding and application of ELISA. This Edition enlarges on charting methods for assessing the indirect ELISA, ruggedness and robustness of tests, aspects of kit use and validation, and internal quality control and external quality management of data. The author describes each method in great detail to ensure experimental success and includes advice on equipment choice, maintenance, and calibration. Wherever possible, helpful written explanations are provided along with copious diagrams. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook, Second Edition offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply ELISA methodology successfully in day-to-day basic and clinical research. |
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publisher: Wiley, published: 2002-05-27 ASIN: 0943088399 EAN: 9780943088396 sales rank: 199307 Biochemistry & Molecular Biology of Plants is a major contribution to the plant sciences literature, superbly edited by three distinguished scientists, Bob B. Buchanan, Wilhelm Gruissem, and Russell L. Jones, with contributions from more than 50 world-renowned scientists. With over 1,400 pages, 1,100 full-colour original drawings and 500 photographs this work qualifies, in the words of the Science reviewer, as: "an essential reference for practicing plant biologists and for the increasing number of scientists from other disciplines who are entering the field of plant biology. To exploit fully the wealth of new information provided by the genome projects and to integrate the metabolic, regulatory, and signal transduction pathways of complex organisms, biologists will require a solid command of biochemistry and physiology. For this reason, the publication of Biochemistry Molecular Biology of Plants could not have come at a more opportune and auspicious time." |
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publisher: Springer, published: 2002-06-01 ASIN: 0896039056 EAN: 9780896039056 sales rank: 746164 Barry W. Hicks and a team of well-practiced experimentalists demonstrate the power and the versatility of the green fluorescent protein (GFP) marker with a cutting-edge collection of readily reproducible GFP techniques. These include methods for detecting and imaging GFP (FRET, BRET, and whole-body imaging); for using GFP to create and select for transgenic organisms, for its use as a biosensor; and for studying viruses. |