Related books on 'protein-purification'
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publisher: International University Line, published: 2009-04-15 ASIN: 0972077448 EAN: 9780972077446 sales rank: 1227528 Completely revised and updated, Protein Crystallization, 2nd Edition is a greatly expanded follow-up to the best-selling 1st edition. Completely new chapters on high-throughput methods, mass spectrometry, microcalorimetry, counterdiffusion, heavy-atom derivatization, selenomethionine-labeling, rational strategies for crystallization, and protein modification to improve crystallization. Updated chapters on formulation of the protein before crystallization, characterization of the protein by dynamic light scattering, classic methods and the phase diagram, seeding, and cryoprotection of the crystals. Laboratory exercises are included which can be used for teaching purposes in protein crystallization or crystallography courses. The exercises have been tested in the Practical Protein Crystallization courses that have been running since 1994 at Uppsala University (Sweden), as well as courses elsewhere. The book is divided into four separate sections: Methods, Tools and Strategies, Laboratory Exercises, and an A-Z Glossary. There are 30 color plates for identifying phenomena found in crystallization drops. As in the first edition, the emphasis of the book is on practical problems and questions encountered at the lab bench. |
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by: Dennis Sherwood publisher: Oxford University Press, USA, published: 2010-12-30 ASIN: 019955904X EAN: 9780199559046 sales rank: 438064 A complete account of the theory of the diffraction of x-rays by crystals with particular reference to the processes of determining the structures of protein molecules, this book is aimed primarily at structural biologists and biochemists but will also be valuable to those entering the field with a background in physical sciences or chemistry. It may be used at any post-school level, and develops from first principles all relevant mathematics, diffraction and wave theory, assuming no mathematical knowledge beyond integral calculus. The book covers a host of important topics in the area, including: - The practical aspects of sample preparation and x-ray data collection, using both laboratory and synchrotron sources - Data analysis at both theoretical and practical levels - The important role played by the Patterson function in structure analysis by both molecular replacement and experimental phasing approaches - Methods for improving the resulting electron density map - The theoretical basis of methods used in refinement of protein crystal structures - In-depth explanation of the crucial task of defining the binding sites of ligands and drug molecules - The complementary roles of other diffraction methods which reveal further detail of great functional importance in a crystal structure. |
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publisher: Springer, published: 2002-06-01 ASIN: 0896039056 EAN: 9780896039056 sales rank: 732999 Barry W. Hicks and a team of well-practiced experimentalists demonstrate the power and the versatility of the green fluorescent protein (GFP) marker with a cutting-edge collection of readily reproducible GFP techniques. These include methods for detecting and imaging GFP (FRET, BRET, and whole-body imaging); for using GFP to create and select for transgenic organisms, for its use as a biosensor; and for studying viruses. |
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publisher: Academic Press, published: 1990-03-14 ASIN: 0122135857 EAN: 9780122135859 sales rank: 306575 The Guide to Protein Purification is a self-contained volume covering all the important procedures for purifying proteins, as well as other more specialized techniques. However, to stay within the confines of a single volume, some details are dealt with by reference to other works, but these have been kept to a minimum. It is hoped that this volume will satisfy the needs of both the novice in protein purification and the more experienced researcher. Key Features * Contains procedures newly written for this volume * Provides extensive practical information * Clearly describes rationale and strategies for protein and enzyme purification * Offers personal perspectives on enzyme purification by eminent researchers |
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publisher: Oxford University Press, USA, published: 1990-02-01 ASIN: 0199630038 EAN: 9780199630035 sales rank: 1751858 The first book of a two volume treatise on protein purification methods and applications, this title covers both traditional and recently developed techniques for initial purification, which is fundamental to any investigation of protein chemistry. The theory and basic principles of each technique are discussed, including initial planning, clarification and extraction, concentration of the extract, structure-based separation, activity-based purification, and gel permeation chromatography. Problem areas and solutions are highlighted at each stage, making this book equally valuable to novice researchers and more experienced protein biochemists who will find it a reliable and readily accessible bench manual. |
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by: Scott M. Wheelwright publisher: Wiley-Interscience, published: 1993-11-04 ASIN: 0471037230 EAN: 9780471037231 sales rank: 3153130 A comprehensive introduction to the art and science used by the biochemist and process engineer in the design and optimization of large-scale protein-purification processes. Covers the basics of proteins' properties, the unit operations involved in protein purification as well as important related issues of process design, integration with upstream operations, cost and timing to market. Includes an abundance of figures and tables. |
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publisher: CRC Press, published: 2003-02-05 ASIN: 0824707265 EAN: 9780824707262 sales rank: 3232178 This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing. |
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publisher: Academic Press, published: 1994-11-21 ASIN: 0127255508 EAN: 9780127255507 sales rank: 3529890 A Practical Guide to Membrane Protein Purification is written especially for researchers who have some familarity with separation of water-soluble proteins, but who may not be aware of the pitfalls they face with membrane proteins. This guide presents techniques in a concise form, emphasizing the aspects unique to membrane proteins. The book explains the principles of the methods, permitting researchers and students new to this area to adapt these techniques to their particular needs. The second volume in the series, this book is an essential manual for investigations of structure and function of native membrane proteins, as well as for purification of these proteins for immunization and protein sequencing. Separation, Detection, and Characterization of Biological Macromolecules is a new series of laboratory guides. Each volume focuses on a topic of central interest to scientists and students in biomedical and biological research. Introductory chapters are followed by clear, step-by-step protocols that present principles and practice. These concise manuals are designed for optimal understanding of methods as well as for practical benchtop use. Key Features * Provides general guidelines and strategies for isolation of membrane proteins * Describes detailed practical procedures that have been the widest applications, and lowest specialized equipment needs * Gives special emphasis to new native and denaturing electrophoresis techniques * Explains modifications of techniques used for water-soluble proteins |
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publisher: Academic Press, published: 2000-10-16 ASIN: 0121822273 EAN: 9780121822279 sales rank: 3586620 The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. |
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publisher: Oxford University Press, USA, published: 1990-08-09 ASIN: 0199630232 EAN: 9780199630233 sales rank: 3882859 Here is a new companion volume to Protein Purification Methods: A Practical Approach. It presents several common applications for both large and small scale protein purification methods, and, if used in conjunction with the earlier volume, will provide a complete, easy-to-use guide that will be of equal value to beginners as well as experts in this important area of research. |