Related books on 'flow-cytometry'
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publisher: Wiley-Liss, published: 1993-03-25 ASIN: 0471596345 EAN: 9780471596349 sales rank: 2475524 Handbook of Flow Cytometry Methods Edited By J. Paul Robinson Associate Editors: Zbigniew Darzynkiewicz, Phillip Dean, Lynn Dressler, Hans Tanke and Leon Wheeless Here is the ideal laboratory manual for investigators involved in research and diagnostic applications of flow cytometry. Handbook of flow cytometry methods offers a collection of proven, basic procedures designed for use in the flow cytometry laboratory. Methods implemented at Purdue University Cytometry Laboratories and developed in laboratories of contributors worldwide provide practical, step-by-step procedures for: * the preparation and handling of standards,reagents and specimens * laboratory analyses for immunophenotyping * measurement of DNA content and cell cycle analyses * determination of cell physiology and function * set up and operation of flow cytometers * computational and data analysis techniques * safety precautions Drawing on their collective experience at leading research centers, the editors have fashioned a unique "working manual" intended to serve as the starting point for the evaluation and establishment of laboratory benchmark standards in flow and image cytometry. Each method is described in detail to be used and modified depending on the user's needs. Special features of Handbook of Flow Cytometry Methods include: * ample space throughout for personal annotations * suggestions of alternative procedures; trouble-shooting tips * extensive list of reagents and suppliers * comb-bound format for ease of use at the lab bench Handbook of Flow Cytometry Methods is an essential laboratory resource for students, faculty, and researchers in cell and molecular biology, immunology, cancer biology, hematology/oncology, and laboratory medicine. |
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publisher: CRC Press, published: 1995-11-14 ASIN: 0824796144 EAN: 9780824796143 sales rank: 5521698 This work present practical, biotechnological applications of flow cytometry techniques for the study of animal, plant and microbial cells, explaining methodologies for sample preparation, staining and analysis. It discusses cell variability in cell culture processes and shows how the quantitative analysis of heterogeneous populations aids in the biotechnological exploitation of cells. |
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by: Andrew Yen publisher: CRC Press, published: 1989-08-31 ASIN: 084934834X EAN: 9780849348341 sales rank: 5287814 The current technology and its applications in flow cytometry are presented in this comprehensive reference work. Described in explicit detail are the instrumentation and its components, and applications of the technology in cell biology, immunology, pharmacology, genetics, hematology and clinical medicine. Methods for data analysis, including both hardware and software, and explicit experimental techniques for making specific measurements are presented. Material is divided by topic into two volumes: Volume I covers instrumentation, genetics, and cell structure; Volume II contains material on cell function studies by flow cytometry. This reference is essential for both the novice and the experienced investigator using flow cytometry in research, and for students of cell biology, biomedical engineering, and medical technology. |
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by: David F., Md Keren publisher: Amer Society of Clinical, published: 1994-02 ASIN: 0891893466 EAN: 9780891893462 sales rank: 4239283 ...a comprehensive source on the basic and clinical applications of flow cytometry...balances its clinical advice with theoretical coverage of the diagnostic implications of flow-cytometric & surface-marker analysis. |
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publisher: Springer, published: 2010-12-10 ASIN: 9048163684 EAN: 9789048163687 sales rank: 4209133 Flow cytometry has rapidly evolved into a technique for rapid analysis of DNA content, cellular marker expression and electronic sorting of cells of interest for further investigations. Flow cytometers are being extensively used for monitoring of cellular DNA content, phenotype expression, drug transport, calcium flux, proliferation and apoptosis. Phenotypic analysis of marker expression in leukemic cells has become an important tool for diagnostic and therapeutic monitoring of patients. Recent studies have explored the use of flow cytometry for monitoring hormone receptor expression in human solid tumors and for studies in human genomics. Contributions in the current volume are based on presentations made at the First Indo-US workshop on Flow Cytometry in which experts from USA, UK and India discussed applications of flow cytometry in biological and medical research. This book will be of interest to post graduates and researchers in the fields of pathology, cytology, cell biology and molecular biology. |
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by: Kenneth A., M.D. Ault publisher: New York Academy of Sciences, published: 1993-04 ASIN: 0897667670 EAN: 9780897667678 sales rank: 8992479 |
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by: Roger S. Riley publisher: Igaku-Shoin Medical Pub, published: 1993-04 ASIN: 0896402002 EAN: 9780896402003 sales rank: 2376950 Offers useful, insightful, & up-to-date information on how to establish a flow cytometric lab, how to perform flow cytometric techniques, when to perform flow cytometric analysis, & how to interpret & use flow cytometric data. |
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publisher: Academic Press, published: 2009-11-09 ASIN: 0123750458 EAN: 9780123750457 sales rank: 1343934
* Highlights top downloaded and cited chapters, authored by pioneers in the field and enhanced with their tips, and pitfalls to avoid. * Loaded with detailed protocols developed and used by leaders in the field. *Refines, organizes and updates popular methods from one of our top selling series, Methods in Cell Biology |
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by: Tsieh Sun MD publisher: Lippincott Williams & Wilkins, published: 2002-03-18 ASIN: 0781729645 EAN: 9780781729642 sales rank: 2554233 This thoroughly updated Second Edition provides a comprehensive review of the basic principles, clinical applications, and interpretation of flow cytometry results. Focusing on hematologic neoplasms, it covers current uses and applications while considering cost containment trends in healthcare. More than 30 complete clinical cases with detailed clinical histories, flow cytometric findings, morphologic illustrations, and other pertinent information on molecular biological techniques, cytochemistry, and cytogenetics provide readers with complete clinical information to assist in diagnosis, as well as to hone skills. The text also features 6 new leukemias/lymphomas and 94 new color illustrations, including color photographs of peripheral blood smears, bone marrow smears, bone marrow core biopsies, and histologic sections of lymph nodes, spleen, liver and solid tumors.
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publisher: Wiley-Liss, published: 2000-04-15 ASIN: 0471239577 EAN: 9780471239574 sales rank: 689652 Immunophenotyping Edited by Carleton C. Stewart and Janet K. A. Nicholson In the last twenty years there has been an explosion of immunophenotyping applications using flow cytometry in the clinical laboratory. Immunophenotyping offers essential information for clinicians and laboratorians about the uses of flow cytometers, identifying abnormalities in a variety of disorders, tools of immunophenotyping, assessing platelets in disease states, and much more. This second volume in the new series, Cytometric Cellular Analysis, comprises all cytometric methods currently used to study cellular function through reviews of the principles, theoretical background, and applications of these methods with particular reference to their use in clinical laboratories. Cellular analytical technologies have revolutionized our ability to identify, isolate, and functionally characterize single cells. This volume addresses one of the most important aspects of flow cytometry as it applies to the clinical setting. In addition, this comprehensive book: * Discusses methods used for quality controlling immunophenotyping trials * Reviews various approaches to the use of flow cytometers to quantify fluorescence * Explains how cell surface receptors differentiate between normal and abnormal tissues for diseases including lymphoma, leukemia, and AIDS * Describes a new, sensitive flow cytometric immunophenotyping assay for cross-matching in the case of transplants Imraunophenotyping conveys to researchers, lab supervisors, and scientists working in the areas of flow cytometry, cytology, pathology, hematotogy, immunology, and immunopathology why this outstanding technology is so vital to biomedical research. |