protein-purification books

Related books on 'protein-purification'

Return to life science book list
Number of products: 5223
Page 4 of 523

Protein Purification: From Molecular Mechanisms to Large-Scale Processes (Acs Symposium Series)

publisher: American Chemical Society, published: 1990-05-05
ASIN: 0841217904
EAN: 9780841217904
sales rank: 4618073
This practical volume gives real-life examples of how separation techniques are used in industry with recombinant proteins and emphasizes which techniques might be more useful than others. An introductory chapter ties together the various topics and associates the methods used with examples of large-scale methodologies. The emphasis of the volume is on how series can be applied to developing strategies and appropriate approaches for difficult protein separation problems. This valuable book provides an update on some of the existing technology used in industry and presents separation examples of several commercially important and recently developed proteins.

Protein Purification Applications: A Practical Approach (Practical Approach Series)

publisher: Oxford University Press, USA, published: 2001-04-05
ASIN: 0199636729
EAN: 9780199636723
sales rank: 5478828
Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set) are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.

Membrane Protein Protocols: Expression, Purification, and Characterization (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-10
ASIN: 1617373761
EAN: 9781617373763
sales rank: 4540393
A collection of key techniques for the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocols contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.

Fundamentals of Protein Structure and Function

by: Engelbert Buxbaum
publisher: Springer, published: 2007-09-18
ASIN: 0387263527
EAN: 9780387263526
sales rank: 938214

The textbook is clearly written, and is suitable for advanced students, e.g. during their master and PhD course time. It is very suited also for postdocs and young scientists as a textbook if they change fields in their research. The textbook represents a thorough and detailed description of the topics in each of the chapters. As the book selects very important topics of present fundamental research and relevant in medical biochemistry, it can supplement any of the text books in biochemistry and basic medicine already on the market.

The textbook is clearly written, and is suitable for advanced students, e.g. during their master and PhD course time. It is very suited also for postdocs and young scientists as a textbook if they change fields in their research. The textbook represents a thorough and detailed description of the topics in each of the chapters. As the book selects very important topics of present fundamental research and relevant in medical biochemistry, it can supplement any of the text books in biochemistry and basic medicine already on the market.

Structure in Protein Chemistry

by: Jack Kyte
publisher: Routledge, published: 1995-01-01
ASIN: 0815317018
EAN: 9780815317012
sales rank: 414660
The second edition of "Structure in Protein Chemistry" showcases the latest developments and innovations in the field of protein structure analysis and prediction. The book begins by explaining how proteins are purified and describes methods for elucidating their sequences of amino acids and defining their posttranslational modifications. Comprehensive explanations of crystallography and of noncovalent forces - ionic interactions, hydrogen bonding, and the hydrophobic effect - act as a prelude to an exhaustive description of the atomic details of the structures of proteins. The resulting understanding of protein molecular structure forms the basis for discussions of the evolution of proteins, the symmetry of the oligomeric associations that produce them, and the chemical, mathematical, and physical basis of the techniques used to study their structures.

Protein Methods

by: Daniel M. Bollag
publisher: Wiley-Liss, published: 1996-01-15
ASIN: 0471118370
EAN: 9780471118374
sales rank: 1487317
Protein Methods
Daniel M. Bollag/ Michael D. Rozycki /Stuart J. Edelstein
Reviews from the first edition
"...it is very well written...would certainly be of use to undergraduate and postgraduate students entering the complex world of protein preparation and to more experienced scientists entering this field for the first time."-Biotechnology and Applied Biochemistry
"The book presents these topics with loads of practical detail, so that the reader has little need to consult other reference sources to carry out the techniques described. All in all, a useful book..." -Theoretical & Applied Genetics
"...clearly written with protocols that are easy to follow and the text is well spread-out and easy to read...all methods are fully referenced...a useful book for beginners at a reasonable price." -FEBS Letters
This revised and expanded Second Edition of Protein Methods remains the first source for a complete summary of tested and proven protein techniques. Now divided into two parts, the book begins with the essential chapters from the first edition, updated to reflect important changes in methodology. The chapter on protein isolation includes a new section focusing on the isolation of proteins from inclusion bodies. In the second section, four new chapters are devoted to protein purification and crystallization.
Chapters include
* Preparation for Protein Isolation
* Protein Extraction and Solubilization
* Protein Concentration Determination
* Concentrating Protein Solutions
* Gel Electrophoresis Under Denaturing Conditions
* Gel Electrophoresis Under
...and feature
* reagent items and equipment required for each method
* chapter "view-at-a-glance" tabs
* illustrations for all gel applications and other methods
* complete supplier list
* appendices of basic chemical parameters and molecular data
* key references
* descriptive tables
* Safety notes.

Manufacturing of Pharmaceutical Proteins: From Technology to Economy

by: Stefan Behme
publisher: Wiley-VCH, published: 2009-03-17
ASIN: 3527324445
EAN: 9783527324446
sales rank: 710667
This comprehensive introduction covers all aspects of biopharmaceutical manufacturing, including technology and facilities, GMP and regulatory issues, as well as costing and timeline aspects for supply chain implementation.
Written by a leading expert at one of the largest pharmaceutical companies worldwide, this practical text is aimed at a wide audience ranging from libraries, biotech companies to students and technicians planning to enter biopharmaceutical manufacturing. In addition, it is well suited for academic teaching as well as internal training within larger biotech or pharmaceutical companies.

Protein Phosphatase Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-19
ASIN: 1617377333
EAN: 9781617377334
sales rank: 1002566

Protein Phosphatase Protocols presents a broad range of protocols for the study of protein phosphatases, all written by experts and innovators from phosphatase laboratories around the world. This volume is a compendium of resources for the study of protein phosphatases and their potential as drug targets. Experimental methodologies are taken from proteomics, bioinformatics, genomics, biochemistry, RNAi, and genetics.

Therapeutic Proteins: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2005-08-15
ASIN: 1588293904
EAN: 9781588293909
sales rank: 2044226
World-class researchers share their innovative and proven techniques for the production of therapeutic proteins downstream of the discovery stage. The methods use a variety of sources for producing therapeutic proteins, including bacterial and yeast expression systems, and insect and mammalian cells, and then cover the purification of the resulting protein using both state-of-the-art and traditional methods, such as those sourced from plasma. Additional chapters offer methods for the characterization of therapeutic proteins throughout the production process, along with methods and strategies for viral inactivation and protein formulation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Therapeutic Peptides and Proteins: Formulation, Processing, and Delivery Systems, Second Edition

by: Ajay K. Banga
publisher: CRC Press, published: 2005-09-14
ASIN: 0849316308
EAN: 9780849316302
sales rank: 2174687
Upon publication of the first edition of Therapeutic Peptides and Proteins ten years ago there were only 19 biotechology medicines on the market. Currently there are more than 100, with at least 400 more in various stages of development. That alone would be grounds for a new edition. Add to that the fact that it is still difficult to find up-to-date, relevant information on protein formulation and/or delivery systems and the need for a new edition is self-evident. This second edition continues the tradition of providing a ready-to-use reference of accurate information, tables, and illustrations.
The book begins with the basics of the origin of recombinant proteins, explores structural and analytical aspects, and moves on to discuss the stability of proteins and peptides. Against this background, the author addresses preformulation and formulation considerations and provides detailed information on important topics such as lyophilization. He goes on to discuss various delivery systems, including controlled release delivery systems and products currently on the market. The book also contains newly added regulatory information and thoroughly investigates therapeutic drugs made from proteins as well as protein-based vaccines. A new chapter on recombinant protein subunit vaccines and their delivery systems has been added.
The work of a single author, the book provides complex information in a simple, elegant, and cohesive style. The author minimizes overlap between coverage and includes extensive cross-referencing that links related topics. Completely revised and updated, packed with cutting-edge information, the second edition once again fills the need for a reference that pulls all the relevant information together into one resource.

Page: 1 2 < > 523

Language for page:
Which server:
Operation:
Product category:
Search parameter:
Search Books:

	Protein Purification: From Molecular Mechanisms to Large-Scale Processes (Acs Symposium Series) publisher: American Chemical Society, published: 1990-05-05 ASIN: 0841217904 EAN: 9780841217904 sales rank: 4618073 This practical volume gives real-life examples of how separation techniques are used in industry with recombinant proteins and emphasizes which techniques might be more useful than others. An introductory chapter ties together the various topics and associates the methods used with examples of large-scale methodologies. The emphasis of the volume is on how series can be applied to developing strategies and appropriate approaches for difficult protein separation problems. This valuable book provides an update on some of the existing technology used in industry and presents separation examples of several commercially important and recently developed proteins.
	Protein Purification Applications: A Practical Approach (Practical Approach Series) publisher: Oxford University Press, USA, published: 2001-04-05 ASIN: 0199636729 EAN: 9780199636723 sales rank: 5478828 Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set) are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.
	Membrane Protein Protocols: Expression, Purification, and Characterization (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-10 ASIN: 1617373761 EAN: 9781617373763 sales rank: 4540393 A collection of key techniques for the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocols contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.
	Fundamentals of Protein Structure and Function by: Engelbert Buxbaum publisher: Springer, published: 2007-09-18 ASIN: 0387263527 EAN: 9780387263526 sales rank: 938214 The textbook is clearly written, and is suitable for advanced students, e.g. during their master and PhD course time. It is very suited also for postdocs and young scientists as a textbook if they change fields in their research. The textbook represents a thorough and detailed description of the topics in each of the chapters. As the book selects very important topics of present fundamental research and relevant in medical biochemistry, it can supplement any of the text books in biochemistry and basic medicine already on the market. The textbook is clearly written, and is suitable for advanced students, e.g. during their master and PhD course time. It is very suited also for postdocs and young scientists as a textbook if they change fields in their research. The textbook represents a thorough and detailed description of the topics in each of the chapters. As the book selects very important topics of present fundamental research and relevant in medical biochemistry, it can supplement any of the text books in biochemistry and basic medicine already on the market.
	Structure in Protein Chemistry by: Jack Kyte publisher: Routledge, published: 1995-01-01 ASIN: 0815317018 EAN: 9780815317012 sales rank: 414660 The second edition of "Structure in Protein Chemistry" showcases the latest developments and innovations in the field of protein structure analysis and prediction. The book begins by explaining how proteins are purified and describes methods for elucidating their sequences of amino acids and defining their posttranslational modifications. Comprehensive explanations of crystallography and of noncovalent forces - ionic interactions, hydrogen bonding, and the hydrophobic effect - act as a prelude to an exhaustive description of the atomic details of the structures of proteins. The resulting understanding of protein molecular structure forms the basis for discussions of the evolution of proteins, the symmetry of the oligomeric associations that produce them, and the chemical, mathematical, and physical basis of the techniques used to study their structures.
	Protein Methods by: Daniel M. Bollag publisher: Wiley-Liss, published: 1996-01-15 ASIN: 0471118370 EAN: 9780471118374 sales rank: 1487317 Protein Methods Daniel M. Bollag/ Michael D. Rozycki /Stuart J. Edelstein Reviews from the first edition "...it is very well written...would certainly be of use to undergraduate and postgraduate students entering the complex world of protein preparation and to more experienced scientists entering this field for the first time."-Biotechnology and Applied Biochemistry "The book presents these topics with loads of practical detail, so that the reader has little need to consult other reference sources to carry out the techniques described. All in all, a useful book..." -Theoretical & Applied Genetics "...clearly written with protocols that are easy to follow and the text is well spread-out and easy to read...all methods are fully referenced...a useful book for beginners at a reasonable price." -FEBS Letters This revised and expanded Second Edition of Protein Methods remains the first source for a complete summary of tested and proven protein techniques. Now divided into two parts, the book begins with the essential chapters from the first edition, updated to reflect important changes in methodology. The chapter on protein isolation includes a new section focusing on the isolation of proteins from inclusion bodies. In the second section, four new chapters are devoted to protein purification and crystallization. Chapters include * Preparation for Protein Isolation * Protein Extraction and Solubilization * Protein Concentration Determination * Concentrating Protein Solutions * Gel Electrophoresis Under Denaturing Conditions * Gel Electrophoresis Under ...and feature * reagent items and equipment required for each method * chapter "view-at-a-glance" tabs * illustrations for all gel applications and other methods * complete supplier list * appendices of basic chemical parameters and molecular data * key references * descriptive tables * Safety notes.
	Manufacturing of Pharmaceutical Proteins: From Technology to Economy by: Stefan Behme publisher: Wiley-VCH, published: 2009-03-17 ASIN: 3527324445 EAN: 9783527324446 sales rank: 710667 This comprehensive introduction covers all aspects of biopharmaceutical manufacturing, including technology and facilities, GMP and regulatory issues, as well as costing and timeline aspects for supply chain implementation. Written by a leading expert at one of the largest pharmaceutical companies worldwide, this practical text is aimed at a wide audience ranging from libraries, biotech companies to students and technicians planning to enter biopharmaceutical manufacturing. In addition, it is well suited for academic teaching as well as internal training within larger biotech or pharmaceutical companies.
	Protein Phosphatase Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-19 ASIN: 1617377333 EAN: 9781617377334 sales rank: 1002566 Protein Phosphatase Protocols presents a broad range of protocols for the study of protein phosphatases, all written by experts and innovators from phosphatase laboratories around the world. This volume is a compendium of resources for the study of protein phosphatases and their potential as drug targets. Experimental methodologies are taken from proteomics, bioinformatics, genomics, biochemistry, RNAi, and genetics.
	Therapeutic Proteins: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2005-08-15 ASIN: 1588293904 EAN: 9781588293909 sales rank: 2044226 World-class researchers share their innovative and proven techniques for the production of therapeutic proteins downstream of the discovery stage. The methods use a variety of sources for producing therapeutic proteins, including bacterial and yeast expression systems, and insect and mammalian cells, and then cover the purification of the resulting protein using both state-of-the-art and traditional methods, such as those sourced from plasma. Additional chapters offer methods for the characterization of therapeutic proteins throughout the production process, along with methods and strategies for viral inactivation and protein formulation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
	Therapeutic Peptides and Proteins: Formulation, Processing, and Delivery Systems, Second Edition by: Ajay K. Banga publisher: CRC Press, published: 2005-09-14 ASIN: 0849316308 EAN: 9780849316302 sales rank: 2174687 Upon publication of the first edition of Therapeutic Peptides and Proteins ten years ago there were only 19 biotechology medicines on the market. Currently there are more than 100, with at least 400 more in various stages of development. That alone would be grounds for a new edition. Add to that the fact that it is still difficult to find up-to-date, relevant information on protein formulation and/or delivery systems and the need for a new edition is self-evident. This second edition continues the tradition of providing a ready-to-use reference of accurate information, tables, and illustrations. The book begins with the basics of the origin of recombinant proteins, explores structural and analytical aspects, and moves on to discuss the stability of proteins and peptides. Against this background, the author addresses preformulation and formulation considerations and provides detailed information on important topics such as lyophilization. He goes on to discuss various delivery systems, including controlled release delivery systems and products currently on the market. The book also contains newly added regulatory information and thoroughly investigates therapeutic drugs made from proteins as well as protein-based vaccines. A new chapter on recombinant protein subunit vaccines and their delivery systems has been added. The work of a single author, the book provides complex information in a simple, elegant, and cohesive style. The author minimizes overlap between coverage and includes extensive cross-referencing that links related topics. Completely revised and updated, packed with cutting-edge information, the second edition once again fills the need for a reference that pulls all the relevant information together into one resource.