Related books on 'biology-protocols'
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publisher: Humana Press, published: 2010-10-25 ASIN: 1607617439 EAN: 9781607617433 sales rank: 722842 Alzheimer’s disease and frontotemporal dementia, two of the most prevalent neurodegenerative diseases, are dreaded disorders that attack the neural networks underlying memory and personality, systems that make us who we are. Major breakthroughs in recent years have spurred new research approaches to these conditions. In Alzheimer's Disease and Frontotemporal Dementia: Methods and Protocols, expert investigators bring together the many divergent areas of expertise used to study these diseases, including behavior, electrophysiology, confocal microscopy, and hardcore biochemistry. Beginning with an overview of the two diseases and contemporary research on them, the book continues with specialized protocols for working with amyloid-β peptide, tau, and apolipoprotein E as well as experimental systems for studying AD and FTD, including cell and animal models, and outcome measures that can be used to assess neuronal function in these systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Alzheimer's Disease and Frontotemporal Dementia: Methods and Protocols serves as an ideal guide for all scientists pursuing the cause and cure of these widespread and nightmarishly debilitating disorders. |
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publisher: Humana Press, published: 2005-08-15 ASIN: 1588293904 EAN: 9781588293909 sales rank: 2044226 World-class researchers share their innovative and proven techniques for the production of therapeutic proteins downstream of the discovery stage. The methods use a variety of sources for producing therapeutic proteins, including bacterial and yeast expression systems, and insect and mammalian cells, and then cover the purification of the resulting protein using both state-of-the-art and traditional methods, such as those sourced from plasma. Additional chapters offer methods for the characterization of therapeutic proteins throughout the production process, along with methods and strategies for viral inactivation and protein formulation. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. |
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publisher: Springer, published: 2010-06-11 ASIN: 1607617641 EAN: 9781607617648 sales rank: 1650848 The beauty and variety of plants has fascinated mankind for thousands of years, and this fascination continues today as plant developmental biologists discover mechanisms that control the establishment of specialized cell types, tissues, and organs from the fertilized egg during a plant’s life. In Plant Developmental Biology: Methods and Protocols, expert researchers provide a collection of protocols for many of the common experimental approaches in plant developmental biology, including diverse methods that range from grafting over bimolecular fluorescence complementation to chromatin immunoprecipitation. Chapters are divided into six major parts: growth protocols, manipulation of gene activity, assaying developmental phenotypes, assaying gene activity, testing protein-protein interactions, and probing chromatin. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and revolutionary, Plant Developmental Biology: Methods and Protocols is an essential guide for all plant developmental geneticists, biochemists, and researchers in stress physiology and plant nutrition. |
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publisher: Springer, published: 2007-06-12 ASIN: 1588296679 EAN: 9781588296672 sales rank: 302455 This book offers a broad collection of methods for studying the molecular biology, function, and features of mitochondria. In the past decade, mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes. This practical guide presents a wide spectrum of mitochondrial methods, each written by specialists with solid experience and intended for implementation by novice and expert researchers alike. |
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publisher: Humana Press, published: 2010-11-19 ASIN: 1617376973 EAN: 9781617376979 sales rank: 3932713 This book aims to assist research scientists in choosing the most applicable database or bioinformatics tools to aid and promote their research in plant biotechnology. Chapters include practical examples and highlight common problems encountered in bioinformatics analysis. Further chapters are aimed at researchers developing bioinformatics databases and tools, detailing commonly applied database formats and biology-focused scripting languages. |
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by: Bruce Alberts publisher: Garland Science, published: 1994-03 ASIN: 0815316194 EAN: 9780815316190 sales rank: 323992 "The epitome of what a current upper level textbook in cell biology should be". -- Quarterly Review of Biology "Accessible and relatively easy to read....timely and accurate.... profusely and beautifully illustrated". -- American Society for Microbiology News "A reliable, accurate and up-to-date textbook for which students and researchers must be grateful". -- Trends in Biochemical Sciences "The standard against which other scientific textbooks will be measured". -- Biochemical Education Now generously illustrated in full color and featuring many new panels, drawings, and diagrams, the Third Edition is completely reorganized to reflect new discoveries, new emphases, and new approaches. It covers major advances in signal transduction, intracellular protein sorting, gene regulation, control of cell division, and developmental biology. The biology of plants and the nervous system are integrated into the main text. The Third Edition also extensively updates coverage of immunology and cancer, and adds two new chapters that examine recombinant DNA techniques and proteins as machines. |
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publisher: Humana Press, published: 1998-02-24 ASIN: 0896033856 EAN: 9780896033856 sales rank: 1881235 A comprehensive collection of state-of-the-art techniques for generating transgenic plants that are resistant to plant viruses via the cloning and expression of the coat protein gene. Its unfailingly reproducible methods, perfected by hands-on masters, cover the entire process from virus isolation, RNA extraction, and cloning coat protein genes, to the introduction of the coat protein gene into the plant genome and the testing of transgenic plants for resistance. Methods for testing for transformation by PCR and Southern blotting, the detection of RNA transcripts by Northern blotting, and the production of protein by Western analysis are provided, as are methods for challenging the transgenic plants produced and for detecting and measuring the levels of virus. |
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publisher: Humana Press, published: 2010-11-10 ASIN: 1617371513 EAN: 9781617371516 Expert investigators describe in detail the most widely used techniques in chemokine biology. Covering both ligands and receptors, these readily reproducible methods cover all aspects of chemokine research, ranging from the cloning and characterization of chemokines and their receptors, through the use of animal models to study chemokine function in vivo. Each method also includes relevant background information, as well as providing a useful bibliography that renders the study of chemokines accessible at all levels of experience. Comprehensive and highly practical, Chemokine Protocols offers experimental and clinical chemokine researchers today's gold-standard collection of proven methods for analyzing this biologically ubiquitous and important class of proteins. |
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publisher: Humana Press, published: 2010-11-19 ASIN: 1617377155 EAN: 9781617377150 sales rank: 517188 Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design, Volume 1: Isolation, Characterization, and Interactions examines a multitude of topics, including the isolation of phages, morphological and molecular characterization, and interaction with bacteria. Written in the highly successful Methods in Molecular Biology™ series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject. |
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publisher: Humana Press, published: 1999-11-19 ASIN: 0896038521 EAN: 9780896038523 sales rank: 3519425 Drawing on state-of-the-art cellular and molecular techniques as well as new and sophisticated imaging and information technologies, this comprehensive, three-volume collection of cutting-edge protocols provides readily reproducible methods for studying and analyzing the events of embryonic development. Volume 1 (ISBN: 089603-574-3) contains techniques for establishing and characterizing several widely used experimental model systems, for the study of developmental patterns and morphogenesis, and for the examination of embryo structure and function. There are also step-by-step methods for the analaysis of cell lineage, the production and use of chimeras, and the experimental and molecular manipulation of embryos, including the application of viral vectors. Volume 2 (ISBN: 0-89603-575-1) describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. No less innovative, Volume 3 (ISBN: 0-89603-576-X) introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines. |