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publisher: Wiley-Liss, published: 1990-04-30
ASIN: 0471562351
EAN: 9780471562351
sales rank: 2456747
Revised and updated, this Second Edition of a classic text describes and evaluates--in greater detail--the most recent practical applications of flow cytometry technique to basic cellular biological investigations and clinical research on human neoplasms. Ideal for the experienced researcher as well as the novice, this informative book offers state-of-the-art reviews of all aspects of flow cytometry. New articles highlight investigations of higher plants, the flow cytometry of microorganisms, and measurements of intracellular ionized calcium and membrane potential--illustrating techniques of specimen preparation, measurement and analysis for each. New chapters examine applications of flow cytometry to medical genetics, genetic toxicology, and ultrasensitive analysis of molecules in solution. The Second Edition goes beyond the traditional analysis of DNA histograms with BrdU incorporation and DNA denaturability to identify and analyze the cell cycle more precisely. New or rewritten chapters discuss the importance of flow cytometry for measurements of nucleic acids, chromatin, and DNA and cover the cytometry of sperm and the cytopathic effects of viruses.
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by: Rafael Nunez
publisher: Horizon Press, published: 2001-10-02
ASIN: 1898486263
EAN: 9781898486268
sales rank: 6243957
An essential handbook for research scientists that use, or plan to use, flow cytometers. Provides an introduction and guide for those new to the field while serving as a valuable reference for experienced users who want to investigate new applications. Aimed especially at the research scientist wishing to explore the applications of flow cytometry in a wide range of scientific fields, this book covers basic principles, instrumentation, data analysis, molecular cytometry, DNA analysis, and many practical applications. Fully illustrated throughout with a comprehensive index and useful appendices.
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publisher: Academic Pr, published: 1985-08
ASIN: 0127121501
EAN: 9780127121505
sales rank: 3094550
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publisher: Williams & Wilkins, published: 1993-01-01
ASIN: 0683004808
EAN: 9780683004809
sales rank: 3894289
Aimed at pathologists, oncologists, haematologists and laboratory medicine specialists, this book on flow cytometry addresses such topics as fundamental principles, basic techniques and clinical applications, with an emphasis on its relation to the biology of human cancers and other diseases. Chapters on the clinical application of flow cytometry include its use in diagnosing lymphomas and a wide variety of other cancers. Each organ-system chapter is followed by a clinical commentary that provides additional perspectives on the diagnosis utility of this technology.
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publisher: Humana Press, published: 1998-06-15
ASIN: 0896035387
EAN: 9780896035386
sales rank: 4052073
Leading researchers and experimentalists update the field of flow cytometry with detailed accounts of their many new, improved, and well-tested protocols and applications. Among the cutting-edge methods presented here are techniques for the detection of terminal transferase ion leukemia, intracellular cytokines, intracellular/intranuclear antigens in leukemia and tumors, and cyclins. Protocols for performing functional studies include methods for assessing viability, transmembrane potential, oxidative metabolism, environmental particulate uptake, drug resistance, and cellular pharmacokinetics. The methods for examining DNA address cell cycle analysis, DNA/RNA analysis of solid malignancies, solid-tumor DNA ploidy analysis, apoptosis, and fluorescent in situ hybridization. Protocols for chromosome sorting and 5-6 color analysis are included as advanced methods. The techniques in this volume take advantage of the new instrumentation and probes that have recently been developed to advance the field of flow cytometry and improve standard methods.
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by: Rochelle A. Diamond
publisher: Springer, published: 2000-04-13
ASIN: 3540651497
EAN: 9783540651499
sales rank: 3969979
A contribution towards making this increasingly valuable technology accessible to researchers, including the students, post-doctoral scholars, and technicians gathering the knowledge inherent in this integration between analysis and physical isolation/purification methodologies. A step-by-step approach to the methodology for measuring various attributes demonstrated in the particular cells of interest is provided, as is a myriad of resources to fuel the curiosity and answer questions of both new and adept users. This book stems from the editors'experiences managing flow cytometry/cell sorting core facilities for the emerging researchers, in particular in developmental, cellular, and molecular biology.
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by: Japan and Hanamaki-shi, Japan) International Symposium on Flow Cytometry and Image Analysis for Clinical Applications (1990 : Morioka-shi
publisher: Excerpta Medica, published: 1991-06
ASIN: 0444813799
EAN: 9780444813794
The techniques of flow cytometry and their clinical applications have rapidly developed over the last 10 years. Modern instruments of flow cytometry have become reliable and sophisticated in cell analysis and powerful tools in research in cell biology, oncology, immunology and various fields of clinical medicine. This book presents a comprehensive update of ideas and instruments of flow cytometry and image analysis, summarizing the present state of flow cytometry. Papers are presented consisting of valuable research in entire fields of cell biology, cell kinetics, DNA ploidy, surface antibodies and cytogenetics. Moreover, many papers have distinctly foreseeable clinical applications in studies of cancer diagnosis and therapy, immunology and virology.
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publisher: Blackwell Science Inc, published: 1994-08
ASIN: 0632036737
EAN: 9780632036738
sales rank: 6119755
Flow cytometry is a relatively new technique used by haematologists and immunologists for the diagnosis and follow-up of leukaemias, and the investigation of immune profiles in patients with immune deficient disorders, especially AIDS. It is also used in tests for auto-antibodies. This is a practical text for those who need an introduction to the technique.
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by: W. M. Grogan
publisher: CRC Press, published: 1990-07-27
ASIN: 0824783301
EAN: 9780824783303
sales rank: 6561555
Discusses the methodology and procedures used in studies of the cell cycle, cell development and differentiation, ageing, immunology, membrane fluidity, and aneuploidy analysis of the 15 most common forms of cancer. Described techniques of analysis include preparation of single-cell suspensions, DNA
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by: Philippe Vielh
publisher: Igaku-Shoin Medical Pub, published: 1991-07
ASIN: 0896402045
EAN: 9780896402041
sales rank: 10161024
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