Related books on 'protein-purification'
Return to life science book listNumber of products: 5225
Page 5 of 523
|
by: Martin Zacharias publisher: Imperial College Press, published: 2010-05-04 ASIN: 1848163398 EAN: 9781848163393 sales rank: 1247680 Given the immense progress achieved in elucidating protein protein complex structures and in the field of protein interaction modeling, there is great demand for a book that gives interested researchers/students a comprehensive overview of the field. This book does just that. It focuses on what can be learned about protein protein interactions from the analysis of protein protein complex structures and interfaces. What are the driving forces for protein protein association? How can we extract the mechanism of specific recognition from studying protein protein interfaces? How can this knowledge be used to predict and design protein protein interactions (interaction regions and complex structures)? What methods are currently employed to design protein protein interactions, and how can we influence protein protein interactions by mutagenesis and small-molecule drugs or peptide mimetics? The book consists of about 15 review chapters, written by experts, on the characterization of protein protein interfaces, structure determination of protein complexes (by NMR and X-ray), theory of protein protein binding, dynamics of protein interfaces, bioinformatics methods to predict interaction regions, and prediction of protein protein complex structures (docking and homology modeling of complexes, etc.) and design of protein protein interactions. It serves as a bridge between studying/analyzing protein protein complex structures (interfaces), predicting interactions, and influencing/designing interactions. |
|
by: Andrew Brown publisher: Oxford University Press, USA, published: 2007-05-10 ASIN: 0199205655 EAN: 9780199205653 sales rank: 1390058 J.D. Bernal, known as "Sage," was an extraordinary man and multifaceted character. A scientist of dazzling intellectual ability and a leading figure in the development of X-ray crystallography, he was a polymath, a fervent Marxist, and much admired worldwide. Although he himself never won a Nobel Prize, several of his distinguished students went on to do so, including Dorothy Hodgkin, Max Perutz, and Aaron Klug. Andrew Brown has had unprecedented access to Bernal's papers and diaries, and this biography includes previously unpublished material on Bernal's role during the Second World War. Bernal not only changed the course of science, but was witness to (and often a participant in) historical events (the Easter Rebellion, the Great Strike, the anti-fascist movement and pacific causes, civil defense, RAF bombing strategy, the planning for D-Day, post-war rebuilding, and nuclear weapons.) One of the few men familiar with Downing Street, the White House and the Kremlin, he left fascinating accounts of Churchill, Stalin, Mao Zedong, Louis Mountbatten and Picasso, as well as the century's greatest scientists. Brown's compelling account covers all aspects of Bernal's brilliant, colorful, and bohemian life, and introduces this towering figure of 20th century science to a wide audience. |
|
by: M. Kastner publisher: Elsevier Science, published: 1999-12-28 ASIN: 0444502114 EAN: 9780444502117 sales rank: 4648976 A handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes like membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, including coupling reaction, buffer solution additives, and software. The text as a whole can be viewed as a systematic survey of available methods and how best to use them, but also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the overall essence of the volume. The principle philosophy of this compilation is that practical application is everything, therefore both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Over-exposure to history, concept and theory has deliberately been avoided. The reader will find a wealth of tips and tricks from users for users, including advice on the advantages and disadvantages of each method. Easy-to-read sections on "Getting started now" and "Where to go from here" attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area. |
|
publisher: CRC Press, published: 2009-07-28 ASIN: 0824754689 EAN: 9780824754686 sales rank: 4270519 A True Insider’s Guide to the Field – Then and Now Until now, there has not been a book that effectively addresses the historical basis of protein discovery. Featuring contributions from a distinguished international panel of experts, Protein Discovery Technologies elucidates the principles, techniques, strategies, and broad range of applications of protein discovery by documenting the often untold stories and personal accounts of the contributors’ past scientific achievements. Incorporates Short, Personal Discovery Vignettes Rather than an exhaustive field analysis, this globally pertinent resource presents in-depth discussions of various methods for protein discovery, including bioinformatics, interaction cloning, protein purification, phage display, non-primate models, and chemical targeting. It also explores biological themes through the examination of cell death, angiogenesis, hemostasis, development, signal transduction, transcriptional control, cell cycle control, neurobiology, and quality control. This readily accessible resource discusses an array of interesting topics, including:
Whereas most books tend to be more specialized, this book contains a broad view of the protein discoveries in many different fields, making Protein Discovery Technologies a valuable reference for today’s researchers, both the new and more seasoned, who are seeking a newfound perspective or a deeper understanding of this exciting field. Daniel E. Levy, editor of the Drug Discovery Series, is the founder of DEL BioPharma, a consulting service for drug discovery programs. He also maintains a blog that explores organic chemistry. |
|
by: Douglas T. Gjerde publisher: Wiley-VCH, published: 2009-09-08 ASIN: 3527321160 EAN: 9783527321162 sales rank: 1723865 This first book on the market covers the many new and important RNA species discovered over the past five years, explaining current methods for the enrichment, separation and purification of these novel RNAs. Building up from general principles of RNA biochemistry and biophysics, this book addresses the practical aspects relevant to the laboratory researcher throughout, while discussing the performance and potential problems of the methods discussed. An appendix contains a glossary with the important terms and techniques used in RNA analysis. By explaining the basic and working principles of the methods, the book allows biochemists and molecular biologists to gain much more expertise than by simply repeating a pre-formulated protocol, enabling them to select the procedure and materials best suited to the RNA analysis task at hand. As a result, they will be able to develop new protocols where needed and optimize and fine-tune the general purpose standard protocols that come with the purification equipment and instrumentation. |
|
publisher: Wiley-Interscience, published: 2009-02-09 ASIN: 0470209623 EAN: 9780470209622 sales rank: 550452 Traditional column chromatography dominates current purification technology, and many of the productivity gains that have been achieved have relied on upscaling such devices. However, this comes with a cost penalty and the pharmaceutical industry has reached the point at which further upscaling becomes economically unsupportable. This book offers a broad-based reassessment of old and new purification methods, incorporating an analysis of innovative new trends in purification. The book has wide coverage of different antibody purification strategies and brings together top-tier experts to address problems in process-scale antibody purification. |
|
publisher: Humana Press, published: 2010-11-10 ASIN: 1617373389 EAN: 9781617373381 sales rank: 2172971 Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Divided into two sections, Protein Engineering Protocols reviews rational protein design strategies and directed evolutionary techniques, and their impact on protein engineering. The first section presents design and computational strategies for protein engineering focusing on designing a protein with desired properties, including examples covering a wide range of engineering techniques, such as protein-protein interactions, DNA binding, antibody mimics, and enzymatic activity. The second section on evolutionary techniques provides step-by-step instructions on library design and statistical assessment of library quality. New methods for DNA shuffling as well as different selection strategies are also presented. Following the successful Methods in Molecular Biology™ series format, this volume provides a comprehensive guide to the methods used at every stage of the engineering process. By combining a thorough theoretical foundation with detailed protocols, Protein Engineering Protocols will be invaluable to all research workers in the area, from graduate students to senior investigators. |
|
publisher: Humana Press, published: 1996-06-15 ASIN: 0896033368 EAN: 9780896033368 sales rank: 3038687 A comprehensive collection of essential, time-tested recipes for successful protein fractionation and purification in any experimental circumstance. The protocols give step-by-step instructions on how to select a source for the protein of interest, how to obtain a usable initial extract, how to purify the protein from that extract using both chemical and molecular methods, and how to dry and store the purified protein. Protein Purification Protocols provides all that is needed to design and carry out a successful purification program. It helps both experienced and novice investigators to clarify and define their purification problems and then provides a comprehensive set of tools for a practical solution. |
|
publisher: Oxford University Press, USA, published: 1990-11-22 ASIN: 0199630496 EAN: 9780199630493 sales rank: 10950870 This useful volume covers extraction, concentration and buffer exchange, and techniques of separation on the basis of charge, size and exploitation of activity. |
|
|
publisher: University Park Press, published: 1976 ASIN: 0839109245 EAN: 9780839109242 sales rank: 10049079 |