Related books on 'methods-in-molecular-biology'
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Statistics in Human Genetics and Molecular Biology (Chapman & Hall/CRC Texts in Statistical Science) by: Cavan Reilly publisher: Chapman and Hall/CRC, published: 2009-06-19 ASIN: 1420072633 EAN: 9781420072631 sales rank: 1123747 Focusing on the roles of different segments of DNA, Statistics in Human Genetics and Molecular Biology provides a basic understanding of problems arising in the analysis of genetics and genomics. It presents statistical applications in genetic mapping, DNA/protein sequence alignment, and analyses of gene expression data from microarray experiments. The text introduces a diverse set of problems and a number of approaches that have been used to address these problems. It discusses basic molecular biology and likelihood-based statistics, along with physical mapping, markers, linkage analysis, parametric and nonparametric linkage, sequence alignment, and feature recognition. The text illustrates the use of methods that are widespread among researchers who analyze genomic data, such as hidden Markov models and the extreme value distribution. It also covers differential gene expression detection as well as classification and cluster analysis using gene expression data sets. Ideal for graduate students in statistics, biostatistics, computer science, and related fields in applied mathematics, this text presents various approaches to help students solve problems at the interface of these areas. |
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by: Felix Franks publisher: Humana Press, published: 1988-04-18 ASIN: 0896031098 EAN: 9780896031098 sales rank: 904779 |
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publisher: Springer, published: 2006-02-15 ASIN: 1588294986 EAN: 9781588294982 sales rank: 926659 Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells. The second volume, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications. |
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publisher: Humana Press, published: 2011-12-02 ASIN: 1617794147 EAN: 9781617794148 sales rank: 547008 Studies over the past decade have continued to bring tremendous advances to our understanding of bone biology. New pathways have been discovered and expanded our knowledge of the ways in which genes and gene products affect bone cells and thereby bone mass and bone strength. In Bone Research Protocols, Second Edition, expert researchers in the field detail many methods commonly used to study bone biology. Focusing mainly on in vitro methods, this volume gives techniques for isolation, culture and functional analysis of all bone cell types and details a range of imaging methods, including light and ultrastructural microscopy and live cell imaging. Some important in vivo techniques are included, such as analysis of bone resorption and imaging using X rays, fluorescent or luminescent techniques. Methods for study of proteins and nucleic acid are included and methods for analysis of bone composition, measurement of bone strength, and response to mechanical stimulation are described. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bone Research Protocols, Second Edition seeks to aid scientists in the bone field to establish new techniques in their laboratories. |
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by: Russell Schwartz publisher: The MIT Press, published: 2008-07-25 ASIN: 0262195844 EAN: 9780262195843 sales rank: 1096820 A practice-oriented survey of techniques for computational modeling and simulation suitable for a broad range of biological problems. |
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publisher: Humana Press, published: 2010-11-19 ASIN: 1617375322 EAN: 9781617375323 sales rank: 3869898 A collection of classic, novel, and state-of-the-art methods for the study of cell migration in cultured cells, different model organisms, and specialized cells in normal development and disease. Highlights include basic assays that apply to all cell migration studies in vitro, assays in various model organisms, and assays for cancer cells, endothelial cells, and neurons both in vitro and in animal models. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. |
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publisher: Humana Press, published: 2010-11-19 ASIN: 1617377155 EAN: 9781617377150 sales rank: 1694861 Ranging from the evolution of pathogenicity to oceanic carbon cycling, the many and varied roles that bacteriophages play in microbial ecology and evolution have inspired increased interest within the scientific community. Bacteriophages: Methods and Protocols pulls together the vast body of knowledge and expertise from top international bacteriophage researchers to provide both classical and state-of-the-art molecular techniques. With its well-organized modular design, Volume 1: Isolation, Characterization, and Interactions examines a multitude of topics, including the isolation of phages, morphological and molecular characterization, and interaction with bacteria. Written in the highly successful Methods in Molecular Biology™ series format, chapters consist of brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section which details tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Bacteriophages: Methods and Protocols is a valuable reference for experienced bacteriophage researchers as well as an easily accessible introduction for newcomers to the subject. |
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publisher: Humana Press, published: 2010-09-29 ASIN: 160761894X EAN: 9781607618942 sales rank: 293434 In the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications. In Enzyme Stabilization and Immobilization: Methods and Protocols, expert researchers explore the latest developments through detailed laboratory protocols, which address many different theories and techniques in enzyme stabilization. Chapters outline protocols for enzyme stabilization in solutions, including: liposome formation, micelle introduction, crosslinking, and additives. Secondly, the book contain protocols for enzyme stabilization via enzyme immobilization, such as sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Enzyme Stabilization and Immobilization: Methods and Protocols is an essential handbook for all molecular biologists, biochemists, and biomedical and biochemical engineers. |
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by: Frank H. Stephenson publisher: Academic Press, published: 2010-07-12 ASIN: 0123756901 EAN: 9780123756909 sales rank: 613852 A must have for all Biotechnology Students and technicians. It walks readers through the minefield of laboratory calculations, helping to prevent the types of errors which could mean the loss of weeks of work. Key Features: * Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology * Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation * Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: * Updated and increased coverage of real time PCR and the mathematics used to measure gene expression * More sample problems in every chapter for readers to practice concepts |
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publisher: Humana Press, published: 2010-11-10 ASIN: 1617374725 EAN: 9781617374722 sales rank: 5397582 Seasoned practitioners from many leading laboratories describe their best readily reproducible screening strategies for isolating useful clones. These techniques have been optimized for sensitivity, high throughput, and robustness, and are of proven utility for directed evolution purposes. The assays presented use a variety of techniques, including genetic complementation, microtiter plates, solid-phase screens with colorimetric substrates, and flow cytometric screens. An accompanying volume, Directed Evolution Library Creation: Methods and Protocols (ISBN 1-58829-285-1), describes readily reproducible methods for the creation of mutated DNA molecules and DNA libraries. Copy for Both Volumes Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods constitute an extraordinary collection of all the key methods used today for directed evolution research. Described in step-by-step detail to ensure robust experimental results, these methods will enable both newcomers and more experienced investigators to design and implement directed evolution strategies for the engineering of novel proteins. The first volume describes methods for the creation of mutated DNA molecules, or DNA libraries, encoding variants of desired proteins. The second volume describes methods for screening DNA libraries to isolate mutant proteins that exhibit a specified function. |