Related books on 'recombinant-protein'
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publisher: Oxford University Press, USA, published: 2001-04-05 ASIN: 0199636737 EAN: 9780199636730 sales rank: 1569793 Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy. |
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publisher: Springer, published: 2009-06-22 ASIN: 1441902953 EAN: 9781441902955 sales rank: 486776 V(D)J Recombination: for the community of immunologists and developmental biologists, the molecular route by which B and T lymphocytes acquire their unique function of affording adaptive immunity. Yet, for many—from experienced scientists to trainees—it represents a (rather too) sophisticated process whose true insight is excessively demanding. However, when not simply considered as a private ground for a few aficionados, it can be seen as a way of understanding how mature lymphocytes carry on their basic functions. For the group of aficionados—which includes this editor—it is an elegant paradigm featuring many fascinating evolutionary achievements of which the biological world alone has the secret. These include a subtle biochemical principle most likely hijacked some 470 million years ago from an ancestral gene invader and since then cleverly adapted by jawed vertebrates to precisely cleave and rearrange their antigen receptor (Ig and TCR) loci. This invader would itself have assigned the services of the nonhomologous end joining (NHEJ) DNA repair machinery as well as various DNA polymerases or transferases to work in concert with developmental clues in lymphoid cell lineages to generate an immune repertoire and efficient host surveillance while avoiding autoimmunity. |
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publisher: Springer, published: 2005-12-21 ASIN: 0387245316 EAN: 9780387245317 sales rank: 3205981 The rapidly evolving field of protein science has now come to realize the ubiquity and importance of protein-protein interactions. It had been known for some time that proteins may interact with each other to form functional complexes, but it was thought to be the property of only a handful of key proteins. However, with the advent of high throughput proteomics to monitor protein-protein interactions at an organism level, we can now safely state that protein-protein interactions are the norm and not the exception. Thus, protein function must be understood in the larger context of the various binding complexes that each protein may form with interacting partners at a given time in the life cycle of a cell. Proteins are now seen as forming sophisticated interaction networks subject to remarkable regulation. The study of these interaction networks and regulatory mechanism, which I would like to term "systems proteomics," is one of the thriving fields of proteomics. The bird-eye view that systems proteomics offers should not however mask the fact that proteins are each characterized by a unique set of physical and chemical properties. In other words, no protein looks and behaves like another. This complicates enormously the design of high-throughput proteomics methods. Unlike genes, which, by and large, display similar physico-chemical behaviors and thus can be easily used in a high throughput mode, proteins are not easily amenable to the same treatment. It is thus important to remind researchers active in the proteomics field the fundamental basis of protein chemistry. This book attempts to bridge the two extreme ends of protein science: on one end, systems proteomics, which describes, at a system level, the intricate connection network that proteins form in a cell, and on the other end, protein chemistry and biophysics, which describe the molecular properties of individual proteins and the structural and thermodynamic basis of their interactions within the network. Bridging the two ends of the spectrum is bioinformatics and computational chemistry. Large data sets created by systems proteomics need to be mined for meaningful information, methods need to be designed and implemented to improve experimental designs, extract signal over noise, and reject artifacts, and predictive methods need to be worked out and put to the test. Computational chemistry faces similar challenges. The prediction of binding thermodynamics of protein-protein interaction is still in its infancy. Proteins are large objects, and simplifying assumptions and shortcuts still need to be applied to make simulations manageable, and this despite exponential progress in computer technology. Finally, the study of proteins impacts directly on human health. It is an obvious statement to say that, for decades, enzymes, receptors, and key regulator proteins have been targeted for drug discovery. However, a recent and exciting development is the exploitation of our knowledge of protein-protein interaction for the design of new pharmaceuticals. This presents particular challenges because protein-protein interfaces are generally shallow and interactions are weak. However, progress is clearly being made and the book seeks to provide examples of successes in this area. |
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by: David P. Clark BA (honors)Christ's College Cambridge 1973 PhD University of Brsitol (England) 1977 publisher: Academic Cell, published: 2008-09-19 ASIN: 0121755525 EAN: 9780121755522 sales rank: 996975 Unlike most Biotechnology textbooks, Dr. David P. Clark's "Biotechnology" approaches modern Biotechnology from a Molecular Basis, which grew out of the increasing biochemical understanding of physiology. Using straight forward, less-technical jargon, Clark manages to introduce each chapter with a basic concept, that ultimately evolves into a more specific detailed principle. This up-to-date text covers a wide realm of topics including forensics and bioethics using colorful illustrations and concise applications. This book will help readers understand what molecular biotechnology actually is as a scientific discipline, how the research in this area is conducted, and how this technology may impact the future. · Up-to-date text focuses on modern biotechnology with a molecular foundation · Basic concepts followed by more detailed, specific applications · Clear, color illustrations of key topics and concepts · Clearly written without overly technical jargon or complicated examples |
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by: Aidong Zhang publisher: Cambridge University Press, published: 2009-04-06 ASIN: 0521888956 EAN: 9780521888950 sales rank: 2052428 The analysis of protein-protein interactions is fundamental to the understanding of cellular organization, processes, and functions. Recent large-scale investigations of protein-protein interactions using such techniques as two-hybrid systems, mass spectrometry, and protein microarrays have enriched the available protein interaction data and facilitated the construction of integrated protein-protein interaction networks. The resulting large volume of protein-protein interaction data has posed a challenge to experimental investigation. This book provides a comprehensive understanding of the computational methods available for the analysis of protein-protein interaction networks. It offers an in-depth survey of a range of approaches, including statistical, topological, data-mining, and ontology-based methods. The author discusses the fundamental principles underlying each of these approaches and their respective benefits and drawbacks, and she offers suggestions for future research. |
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publisher: Humana Press, published: 2010-11-10 ASIN: 1617374490 EAN: 9781617374494 sales rank: 3536713 This collection of readily reproducible methods using the latest advances in NMR technology includes methods for high-level recombinant protein expression using sophisticated isotopic labeling strategies, TROSY methods for the study of structure and dynamics, and methods for the acquisition and interpretation of residual dipolar coupling data. There are also applications of dynamics measurements on multiple timescales, new developments in NMR data analysis, structure calculation protocols, and solid-state methods. The protocols follow the successful Methods in Molecular Biology™ series format, each offering detailed laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. |
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publisher: Springer, published: 2010-11-19 ASIN: 3642097057 EAN: 9783642097058 Over the last decade it has been shown that orphan G protein-coupled receptors (GPCRs) can be used as targets to discover novel neuropeptides. A dozen neuropeptides have been identified through this approach. Each of these neuropeptides has opened new doors for our understanding of fundamental physiological or behavioral responses. For example the orexins, MCH and ghrelin carry fundamental roles in regulating food intake while neuropeptide S, neuromedin S, the prokineticins and the orexins are major players in modulating sleep and circadian rhythms. The chapters of this book review the latest research in the field, most of them are written by the original discoverers of the respective novel neuropeptide. Emphasis is set not only on their discovery but also on their functional significance. Since many of these neuropeptides are part of drug discovery programs, this book impacts academic as well as pharmaceutical research. |
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publisher: Humana Press, published: 1996-08-19 ASIN: 0896033457 EAN: 9780896033450 sales rank: 2969071 Leading practitioners authoritatively describe the newest and most effective spectrometric techniques for the analysis of proteins and peptides. The areas covered range from the elucidation of primary and secondary protein structure and the rapid identification of proteins using database techniques to methods for sequencing, as well as methods for the quantitative determination of peptides. Other chapters provide detailed information on the analysis of glycoproteins and glycopeptides and on the use of mass spectrometry to probe the interactions of proteins, both covalent and noncovalent. |
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by: Martin Zacharias publisher: Imperial College Press, published: 2010-05-04 ASIN: 1848163398 EAN: 9781848163393 sales rank: 2608614 Given the immense progress achieved in elucidating protein protein complex structures and in the field of protein interaction modeling, there is great demand for a book that gives interested researchers/students a comprehensive overview of the field. This book does just that. It focuses on what can be learned about protein protein interactions from the analysis of protein protein complex structures and interfaces. What are the driving forces for protein protein association? How can we extract the mechanism of specific recognition from studying protein protein interfaces? How can this knowledge be used to predict and design protein protein interactions (interaction regions and complex structures)? What methods are currently employed to design protein protein interactions, and how can we influence protein protein interactions by mutagenesis and small-molecule drugs or peptide mimetics? The book consists of about 15 review chapters, written by experts, on the characterization of protein protein interfaces, structure determination of protein complexes (by NMR and X-ray), theory of protein protein binding, dynamics of protein interfaces, bioinformatics methods to predict interaction regions, and prediction of protein protein complex structures (docking and homology modeling of complexes, etc.) and design of protein protein interactions. It serves as a bridge between studying/analyzing protein protein complex structures (interfaces), predicting interactions, and influencing/designing interactions. |
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by: M. Chance publisher: Wiley, published: 2008-09-22 ASIN: 0470258861 EAN: 9780470258866 sales rank: 2352254 |