Related books on 'methods-in-molecular-biology'
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publisher: Humana Press, published: 2010-11-19 ASIN: 1617377430 EAN: 9781617377433 sales rank: 2432841 At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). This book focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome. |
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publisher: Humana Press, published: 2010-11-19 ASIN: 161737525X EAN: 9781617375255 sales rank: 3222131 A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. |
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publisher: Humana Press, published: 2010-11-10 ASIN: 1617371122 EAN: 9781617371127 Well-versed investigators describe in step-by-step detail a wide range of DNA repair activities, from single act-alone repair proteins to complex repair systems. These practical protocols not only detail the various repair activities found in cells, but also demonstrate the use of DNA repair proteins and systems as reagents in molecular biology and biotechnology. The techniques described here include mutation and polymorphism detection, which are useful in the search for disease genes and drug response genes, as well as for breeding and trait selection in animals and plants. Compact and highly practical, DNA Repair Protocols: Prokaryotic Systems provides expert guidance to both the DNA repair researcher studying the fundamental aspects of DNA repair and the applied researcher in human genetics and biotechnology. |
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by: Geoffrey M. Cooper publisher: Sinauer Associates, Inc., published: 2006-06-01 ASIN: 0878932194 EAN: 9780878932191 sales rank: 247733 The fourth edition of "The Cell" has been thoroughly updated to reflect major advances in the field, including the role of microRNAs in the regulation of gene expression, advances in stem cell biology, as well as Oncogenes and the rational design of new anticancer drugs. While retaining the overall structure and pedagogy that made previous editions so successful, the fourth edition now includes additional chapters on Programmed Cell death and the Maintenance of Adult Tissues. There is also significant new material on topics like Signalling Networks, Cell-Matrix Interactions and Bioinformations to keep "The Cell" fresh, vibrant and perpetually appealing to students. |
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publisher: Humana Press, published: 2009-03-05 ASIN: 1934115495 EAN: 9781934115497 sales rank: 977847 As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs. |
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publisher: Humana Press, published: 2012-02-29 ASIN: 1617795607 EAN: 9781617795602 |
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publisher: ASM Press, published: 2007-09 ASIN: 1555812236 EAN: 9781555812232 sales rank: 948565 This volume is a state-of-the-art revision of the previously published Methods for General and Molecular Bacteriology. The new, expanded edition offers comprehensive coverage of methods and includes archaea and filamentous fungi, in addition to bacteria. |
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publisher: Humana Press, published: 2010-11-19 ASIN: 1617376590 EAN: 9781617376597 Protein Design: Methods and Applications presents the most up-to-date protein design and engineering strategies so that readers can undertake their own projects with a maximum chance of success. The authors present integrated computational approaches that require various degrees of computational complexity, and the major accomplishments that have been achieved in the design and structural characterization of helical peptides and proteins. |
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publisher: Humana Press, published: 2011-05-26 ASIN: 1617378267 EAN: 9781617378263 sales rank: 2042239 Due to the rising threat of tuberculosis and other mycobacterial infections, methods to study the biology of the mycobacteria and to improve diagnostic, therapeutic and preventative reagents are still very much in need. Mycobacteria Protocols, Second Edition updates and refines the methods of the well-received first edition while adding newly developed methods culled from the most cutting-edge research in the field. Ranging from the basics of sub-cellular fractionation to advanced methods using specialized growth conditions and whole genome, transcriptome and proteome analysis, the chapters conform to the Methods in Molecular Biology™ series format, providing step-by-step laboratory protocols, lists of necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Mycobacteria Protocols, Second Edition is the perfect resource to promote and stimulate further research into these intriguing, important and most fractious of bacteria. |
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publisher: Humana Press, published: 2010-12-09 ASIN: 1617375764 EAN: 9781617375767 sales rank: 942405 This book provides key methods, approaches, and strategies to dissect the plant defense response. Addressing methods to identify and characterize plant resistance genes as well as pathogen-associated molecules that trigger the plant defense response, this volume creates a better understanding of the interactions between pathogens and their hosts. This will help to develop better methods for disease control in plants and animals. |