recombinant-protein books

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Guidebook to Protein Toxins and Their Use in Cell Biology (Sambrook & Tooze Publication at Oxford University Press)

publisher: A Sambrook and Tooze Publication at Oxford University Press, published: 1997-07-31
ASIN: 0198599544
EAN: 9780198599548
sales rank: 2402232
Many bacteria, animals, and plants produce toxins that can prove lethal to other organisms. Toxins are a form of "biological warfare" that helps their producer to survive and so confer an evolutionary advantage. They display an extraordinary range of complexity, from the formic acid provided by ants to bacterial proteins composed of thousands of amino acids. This Guidebook considers the more complex protein and peptide toxins and groups them according to their mode of action. Topics covered include: membrane-permeabilizing toxins; toxins affecting signal transduction and protein synthesis; cytoskeleton-affecting toxins; toxins affecting the immune and inflammatory response. This class of biomolecules will be of interest to a wide range of researchers in cell biology, neuroscience, and toxicology.

Sphingolipid Metabolism and Cell Signaling, Part B, Volume 312 (Methods in Enzymology)

publisher: Academic Press, published: 2000-10-30
ASIN: 0121822133
EAN: 9780121822132
sales rank: 4213694
This volume contains information on analyzing sphingolipids, sphingolipid transport and trafficking, and sphingolipid-protein interactions and cellular targets. Its companion Volume 311 presents methods used in studying enzymes of sphingolipid biosynthesis and turnover, including inhibitors of some of these enzymes, genetic approaches, and organic and enzymatic syntheses of sphingolipids and analogs.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Redox Proteomics: From Protein Modifications to Cellular Dysfunction and Diseases (Wiley - Interscience Series on Mass Spectrometry)

publisher: Wiley-Interscience, published: 2006-06-12
ASIN: 0471723452
EAN: 9780471723455
sales rank: 2106091
Methodology and applications of redox proteomics

The relatively new and rapidly changing field of redox proteomics has the potential to revolutionize how we diagnose disease, assess risks, determine prognoses, and target therapeutic strategies for people with inflammatory and aging-associated diseases. This collection brings together, in one comprehensive volume, a broad array of information and insights into normal and altered physiology, molecular mechanisms of disease states, and new applications of the rapidly evolving techniques of proteomics.

Written by some of the finest investigators in this area, Redox Proteomics: From Protein Modifications to Cellular Dysfunction and Diseases examines the key topics of redox proteomics and redox control of cellular function, including:
* The role of oxidized proteins in various disorders
* Pioneering studies on the development of redox proteomics
* Analytical methodologies for identification and structural characterization of proteins affected by oxidative/nitrosative modifications
* The response and regulation of protein oxidation in different cell types
* The pathological implications of protein oxidation for conditions, including asthma, cardiovascular disease, diabetes, preeclampsia, and Alzheimer's disease

Distinguished by its in-depth discussions, balanced methodological approach, and emphasis on medical applications and diagnosis development, Redox Proteomics is a rich resource for all professionals with an interest in proteomics, cellular physiology and its alterations in disease states, and related fields.

Protein and Peptide Analysis by Mass Spectrometry (Methods in Molecular Biology)

publisher: Humana Press, published: 1996-08-19
ASIN: 0896033457
EAN: 9780896033450
sales rank: 2041504
Leading practitioners authoritatively describe the newest and most effective spectrometric techniques for the analysis of proteins and peptides. The areas covered range from the elucidation of primary and secondary protein structure and the rapid identification of proteins using database techniques to methods for sequencing, as well as methods for the quantitative determination of peptides. Other chapters provide detailed information on the analysis of glycoproteins and glycopeptides and on the use of mass spectrometry to probe the interactions of proteins, both covalent and noncovalent.

Biosystems Engineering II: Linking Cellular Networks and Bioprocesses (Advances in Biochemical Engineering Biotechnology)

publisher: Springer, published: 2010-09-02
ASIN: 3642138659
EAN: 9783642138652
sales rank: 3038548
-Morphology of Filamentous Fungi: Linking Cellular Biology to Process Engineering Using Aspergillus niger, By Rainer Krull, Christiana Cordes, Harald Horn, Ingo Kampen, Arno Kwade, Thomas R. Neu, and Bernd Nörtemann; -Multi-Scale Spatio-Temporal Modeling: Lifelines of Microorganisms in Bioreactors and Tracking Molecules in Cells, By Alexei Lapin, Michael Klann, and Matthias Reuss; -Impact of Profiling Technologies in the Understanding of Recombinant Protein Production, By Chandran Vijayendran and Erwin Flaschel -Engineering the Escherichia coli Fermentative Metabolism, By M. Orencio-Trejo, J. Utrilla, M.T. Fernàndez-Sandoval, G. Huerta-Beristain, G. Gosset, and A. Martinez; -Modeling Languages for Biochemical Network Simulation: Reaction vs Equation Based Approaches, By Wolfgang Wiechert, Stephan Noack, and Atya Elsheikh; -Impact of Thermodynamic Principles in Systems Biology, By J.J. Heijnen;

Protein Microarrays

by: Mark Schena
publisher: Jones and Bartlett Publishers, Inc., published: 2004-07
ASIN: 0763731277
EAN: 9780763731274
sales rank: 2648922
Protein Microarrays is one of the first books in this exciting new technology and will be an essential reference for most biologists and biotechnology professionals.

Downstream Processing of Proteins: Methods and Protocols (Methods in Biotechnology)

publisher: Humana Press, published: 2010-11-19
ASIN: 1617370754
EAN: 9781617370755
sales rank: 5162679
Mohamed A. Desai and a team of experienced biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. These techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods from academia with a potential for scale-up. Also treated are various strands of the downstream biological process essential for a successful product license application, including both the validation of DSP stages, and the design and validation of viral clearance stages during the purification process. Downstream Processing of Proteins: Methods and Protocols provides scientists everywhere, but particularly in the biopharmaceutical and biotechnology industry, with a much-needed introduction to this critical technology.

Calcium-Binding Protein Protocols: Volume 2: Methods and Techniques (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-10
ASIN: 161737136X
EAN: 9781617371363
Blurb for Volume 2 Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant class proteins, and provide a comprehensive collection of proven experimental techniques for studying it both in vitro and in vivo. This second volume focuses on cutting-edge experimental techniques for studying the solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding protein in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, there are also NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, and fluorescence methods to study the distribution of calcium and calcium-binding proteins in cells. The first companion volume, Reviews and Case Histories sets the stage for this volume by introducing the various classes of intra- and extra-cellular calcium-binding proteins and their mode of action. Single Blurb for Both Volumes Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant protein class, and provide a comprehensive collection of proven experimental techniques for their study both in vitro and in vivo. The first volume discusses the role of calcium in intracellular secondary messenger activation mechanisms, including unique aspects of calcium chemistry and its utilization in dairy proteins and blood clotting. Detailed case studies provide a wealth of valuable information about protein purification and characterization strategies, X-ray crystallography, and specific calcium-binding proteins and their modes of action. The second volume focuses on cutting-edge experimental techniques for studying the solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding protein in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, there are also NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, and fluorescence methods to study the distribution of calcium and calcium-binding proteins in cells.

Posttranslational Modification of Proteins: Tools for Functional Proteomics (Methods in Molecular Biology)

publisher: Humana Press, published: 2002-04-15
ASIN: 0896036782
EAN: 9780896036789
sales rank: 2481753
Christoph Kannicht and a panel of highly experienced researchers describe readily reproducible methods for detecting and analyzing the posttranslational modifications of protein, particularly with regard to protein function, proteome research, and the characterization of pharmaceutical proteins. Among the methods presented are those for analyzing the assignment of disulfide bond sites in proteins, protein N-glycosylation and protein O-glycosylation, and oligosaccharides present at specific single glycosylation sites in a protein. Additional powerful techniques facilitate the analysis of glycosylphosphatidylinositols, lipid modifications, protein phosphorylation and sulfation, protein methylation and acetylation, a-amidation, g-glutamate, isoaspartate, and lysine hydroxylation.

Membrane Protein Protocols: Expression, Purification, and Characterization (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-10
ASIN: 1617373761
EAN: 9781617373763
sales rank: 4539931
A collection of key techniques for the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocols contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.

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	Guidebook to Protein Toxins and Their Use in Cell Biology (Sambrook & Tooze Publication at Oxford University Press) publisher: A Sambrook and Tooze Publication at Oxford University Press, published: 1997-07-31 ASIN: 0198599544 EAN: 9780198599548 sales rank: 2402232 Many bacteria, animals, and plants produce toxins that can prove lethal to other organisms. Toxins are a form of "biological warfare" that helps their producer to survive and so confer an evolutionary advantage. They display an extraordinary range of complexity, from the formic acid provided by ants to bacterial proteins composed of thousands of amino acids. This Guidebook considers the more complex protein and peptide toxins and groups them according to their mode of action. Topics covered include: membrane-permeabilizing toxins; toxins affecting signal transduction and protein synthesis; cytoskeleton-affecting toxins; toxins affecting the immune and inflammatory response. This class of biomolecules will be of interest to a wide range of researchers in cell biology, neuroscience, and toxicology.
	Sphingolipid Metabolism and Cell Signaling, Part B, Volume 312 (Methods in Enzymology) publisher: Academic Press, published: 2000-10-30 ASIN: 0121822133 EAN: 9780121822132 sales rank: 4213694 This volume contains information on analyzing sphingolipids, sphingolipid transport and trafficking, and sphingolipid-protein interactions and cellular targets. Its companion Volume 311 presents methods used in studying enzymes of sphingolipid biosynthesis and turnover, including inhibitors of some of these enzymes, genetic approaches, and organic and enzymatic syntheses of sphingolipids and analogs. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
	Redox Proteomics: From Protein Modifications to Cellular Dysfunction and Diseases (Wiley - Interscience Series on Mass Spectrometry) publisher: Wiley-Interscience, published: 2006-06-12 ASIN: 0471723452 EAN: 9780471723455 sales rank: 2106091 Methodology and applications of redox proteomics The relatively new and rapidly changing field of redox proteomics has the potential to revolutionize how we diagnose disease, assess risks, determine prognoses, and target therapeutic strategies for people with inflammatory and aging-associated diseases. This collection brings together, in one comprehensive volume, a broad array of information and insights into normal and altered physiology, molecular mechanisms of disease states, and new applications of the rapidly evolving techniques of proteomics. Written by some of the finest investigators in this area, Redox Proteomics: From Protein Modifications to Cellular Dysfunction and Diseases examines the key topics of redox proteomics and redox control of cellular function, including: * The role of oxidized proteins in various disorders * Pioneering studies on the development of redox proteomics * Analytical methodologies for identification and structural characterization of proteins affected by oxidative/nitrosative modifications * The response and regulation of protein oxidation in different cell types * The pathological implications of protein oxidation for conditions, including asthma, cardiovascular disease, diabetes, preeclampsia, and Alzheimer's disease Distinguished by its in-depth discussions, balanced methodological approach, and emphasis on medical applications and diagnosis development, Redox Proteomics is a rich resource for all professionals with an interest in proteomics, cellular physiology and its alterations in disease states, and related fields.
	Protein and Peptide Analysis by Mass Spectrometry (Methods in Molecular Biology) publisher: Humana Press, published: 1996-08-19 ASIN: 0896033457 EAN: 9780896033450 sales rank: 2041504 Leading practitioners authoritatively describe the newest and most effective spectrometric techniques for the analysis of proteins and peptides. The areas covered range from the elucidation of primary and secondary protein structure and the rapid identification of proteins using database techniques to methods for sequencing, as well as methods for the quantitative determination of peptides. Other chapters provide detailed information on the analysis of glycoproteins and glycopeptides and on the use of mass spectrometry to probe the interactions of proteins, both covalent and noncovalent.
	Biosystems Engineering II: Linking Cellular Networks and Bioprocesses (Advances in Biochemical Engineering Biotechnology) publisher: Springer, published: 2010-09-02 ASIN: 3642138659 EAN: 9783642138652 sales rank: 3038548 -Morphology of Filamentous Fungi: Linking Cellular Biology to Process Engineering Using Aspergillus niger, By Rainer Krull, Christiana Cordes, Harald Horn, Ingo Kampen, Arno Kwade, Thomas R. Neu, and Bernd Nörtemann; -Multi-Scale Spatio-Temporal Modeling: Lifelines of Microorganisms in Bioreactors and Tracking Molecules in Cells, By Alexei Lapin, Michael Klann, and Matthias Reuss; -Impact of Profiling Technologies in the Understanding of Recombinant Protein Production, By Chandran Vijayendran and Erwin Flaschel -Engineering the Escherichia coli Fermentative Metabolism, By M. Orencio-Trejo, J. Utrilla, M.T. Fernàndez-Sandoval, G. Huerta-Beristain, G. Gosset, and A. Martinez; -Modeling Languages for Biochemical Network Simulation: Reaction vs Equation Based Approaches, By Wolfgang Wiechert, Stephan Noack, and Atya Elsheikh; -Impact of Thermodynamic Principles in Systems Biology, By J.J. Heijnen;
	Protein Microarrays by: Mark Schena publisher: Jones and Bartlett Publishers, Inc., published: 2004-07 ASIN: 0763731277 EAN: 9780763731274 sales rank: 2648922 Protein Microarrays is one of the first books in this exciting new technology and will be an essential reference for most biologists and biotechnology professionals.
	Downstream Processing of Proteins: Methods and Protocols (Methods in Biotechnology) publisher: Humana Press, published: 2010-11-19 ASIN: 1617370754 EAN: 9781617370755 sales rank: 5162679 Mohamed A. Desai and a team of experienced biotechnologists review both conventional and novel isolation techniques used in industrial applications for the downstream processing of protein molecules. These techniques include primary and secondary separations during the isolation of biomolecules, as well as unique laboratory-scale research methods from academia with a potential for scale-up. Also treated are various strands of the downstream biological process essential for a successful product license application, including both the validation of DSP stages, and the design and validation of viral clearance stages during the purification process. Downstream Processing of Proteins: Methods and Protocols provides scientists everywhere, but particularly in the biopharmaceutical and biotechnology industry, with a much-needed introduction to this critical technology.
	Calcium-Binding Protein Protocols: Volume 2: Methods and Techniques (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-10 ASIN: 161737136X EAN: 9781617371363 Blurb for Volume 2 Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant class proteins, and provide a comprehensive collection of proven experimental techniques for studying it both in vitro and in vivo. This second volume focuses on cutting-edge experimental techniques for studying the solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding protein in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, there are also NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, and fluorescence methods to study the distribution of calcium and calcium-binding proteins in cells. The first companion volume, Reviews and Case Histories sets the stage for this volume by introducing the various classes of intra- and extra-cellular calcium-binding proteins and their mode of action. Single Blurb for Both Volumes Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant protein class, and provide a comprehensive collection of proven experimental techniques for their study both in vitro and in vivo. The first volume discusses the role of calcium in intracellular secondary messenger activation mechanisms, including unique aspects of calcium chemistry and its utilization in dairy proteins and blood clotting. Detailed case studies provide a wealth of valuable information about protein purification and characterization strategies, X-ray crystallography, and specific calcium-binding proteins and their modes of action. The second volume focuses on cutting-edge experimental techniques for studying the solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding protein in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, there are also NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, and fluorescence methods to study the distribution of calcium and calcium-binding proteins in cells.
	Posttranslational Modification of Proteins: Tools for Functional Proteomics (Methods in Molecular Biology) publisher: Humana Press, published: 2002-04-15 ASIN: 0896036782 EAN: 9780896036789 sales rank: 2481753 Christoph Kannicht and a panel of highly experienced researchers describe readily reproducible methods for detecting and analyzing the posttranslational modifications of protein, particularly with regard to protein function, proteome research, and the characterization of pharmaceutical proteins. Among the methods presented are those for analyzing the assignment of disulfide bond sites in proteins, protein N-glycosylation and protein O-glycosylation, and oligosaccharides present at specific single glycosylation sites in a protein. Additional powerful techniques facilitate the analysis of glycosylphosphatidylinositols, lipid modifications, protein phosphorylation and sulfation, protein methylation and acetylation, a-amidation, g-glutamate, isoaspartate, and lysine hydroxylation.
	Membrane Protein Protocols: Expression, Purification, and Characterization (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-10 ASIN: 1617373761 EAN: 9781617373763 sales rank: 4539931 A collection of key techniques for the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be overexpressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocols contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.