recombinant-protein books

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Cell-free Protein Synthesis: Methods and Protocols

publisher: Wiley-VCH, published: 2007-12-25
ASIN: 3527316493
EAN: 9783527316496
sales rank: 2139000
With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.

Handbook of Animal Lectins: Properties and Biomedical Applications

by: David C. Kilpatrick
publisher: Wiley, published: 2000-10-31
ASIN: 047189981X
EAN: 9780471899815
sales rank: 876363
This comprehensive yet concise guide to animal lectins,
covers all sources from unicellular protozoa and slime
moulds through invertebrates to mammals and birds, and
provides essential information to the widest possible
readership.

The last few years have seen an explosion of interest and
research in animal lectins, and this book, which is divided
into two parts, provides a compendium of galectins,
collectins, selectins, pentraxins and other
carbohydrate-binding proteins from throughout the animal
kingdom. The first part introduces animal lectins on both
phylogenetic and structural bases and outlines their key
biomedical applications. The second, and major part, is a
quick reference alphabetical directory listing around 170
lectins. Each lectin entry provides, where known, details
of its:

Isolation
Structure
Biological activities
Tissue and/or subcellular distribution
Possible functions
Applications

A bibliography for each lectin is also included and useful
appendices list lectins according to their sugar specificity,
characterised lectins of human origin by tissue source and
commercial suppliers of animal lectins.

This superb book is aimed at basic scientists and clinicians
alike, and will be invaluable to both established research
workers and newcomers to this area. It will be of
particular interest to biochemists, cell biologists,
immunologists, oncologists, pharmacologists, medical
practitioners and students of medical and biological
sciences.

Modern Protein Chemistry: Practical Aspects

publisher: CRC Press, published: 2001-09-12
ASIN: 0849394538
EAN: 9780849394539
sales rank: 3246068
In recent years, interest in proteins has surged. This resurgence has been driven by the expansion of the post-genomic era when structural genomics and proteomics require new techniques in protein chemistry and new applications of older techniques. Protein chemistry methods are used by nearly every discipline of biomedical research. Many techniques have been used in less traditional ways with exciting results. Modern Protein Chemistry: Practical Aspects describes the practical side of advanced techniques in protein chemistry. The book gives researchers an excellent "cost-benefit" analysis of these techniques.

The contributors have been selected for their prominence in their specific fields and because they run laboratories that actively collaborate with other scientists. Researchers and practitioners, both beginners and experienced, who are looking for new ideas and who are interested in applying these more advanced methods will be assisted in their work by these commentaries.

This guide provides hands-on information to complement theoretical understanding. The theory behind these methods can be found in existing books and in the original literature. However, no other guide will help you make a practical evaluation of these methods and their value to your work.

Bioseparations of Proteins, Volume 1: Unfolding/Folding and Validations (Separation Science and Technology)

by: Ajit Sadana
publisher: Academic Press, published: 1997-11-17
ASIN: 0126140405
EAN: 9780126140408
sales rank: 6970109
This book covers the fundamentals of protein inactivation during bioseparation and the effect on protein processing. Bioseparation of Proteins is unique because it provides a background of the bioseparation processes, and it is the first book available to emphasize the influence of the different bioseparation processes on protein inactivation.
Bioseparation of Proteins covers the extent, mechanisms of, and control of protein inactivation during these processes along with the subsequent and essential validation of these processes. The book focuses on the avoidance of protein (biologicalproduct) inactivation at each step in a bioprocess. It compares protein inactivation exhibited during the different bioseparation processes by different workers and provides a valuable framework for workers in different areas interested in bioseparations.
Topics include separation and detection methods; estimates of protein inactivation and an analysis of this problem for different separation processes; strategies for avoiding inactivation; the molecular basis of surface activity and protein adsorption,process monitoring, and product validation techniques; and the economics of various bioseparation processes and quality control procedures.

Key Features
* Protein inactivation and other aspects of biological stability are critical to an effective bioseparation process; This book is a detailed and critical review of the available literature in an area that is essential to the effectiveness, validation, and economics of bioseparation processes for drugs and other biological products; Conveniently assembled under one cover, the survey of the literature and resulting perspective will greatly assist engineers and chemists in designingand improving their own processes; Key features of the text include:
* detailed data on biological stability under various bioseparation conditions
* extensive case studies from the literature on separation processes, validation, and economics
* simplified analysis of protein refolding and inactivation mechanisms
* consideration of adsorption theories and the effect of heterogeneity
* coverage of both classical and novel bioseparation techniques, including chromatographic procedures

Protein Synthesis: Methods and Protocols (Methods in Molecular Biology)

publisher: Humana Press, published: 2010-11-10
ASIN: 1617370258
EAN: 9781617370250
sales rank: 2882724
This book presents a collection of molecular biological methods specific to protein synthesis. Chapters open with a discussion of basic background information and strategy which is then complemented by comprehensive methodological details. The book is divided into seven significant areas that cover all of the research techniques required by both experienced researchers and newcomers to the field of protein synthesis, and will prove to be an invaluable reference source on the benchtop of many protein laboratories.

Cell Surface Receptors, Volume 68 (Advances in Protein Chemistry)

by: K. Christopher Garcia Ph.D
publisher: Academic Press, published: 2004-11-05
ASIN: 0120342685
EAN: 9780120342686
sales rank: 3119365
Cell Surface Receptors contains an extensive discussion of cell surface receptors in 11 chapters by experts in their field. As cell surface receptors are involved in almost every aspect of signaling throughout the body, the topic has been of high interest in the community in recent years.

Selected Contents:
-Structures of Axon Guidance Molecules and Their Neuronal Receptors
-Shared Cytokine Signaling Receptor
-NKG2D and Related Immunoreceptors
-Inonotropic Glutamat Receptor Recognition and Activation
-Chemotaxis Receptors and Signaling

PEGylated Protein Drugs: Basic Science and Clinical Applications (Milestones in Drug Therapy)

publisher: Birkhäuser Basel, published: 2009-10-23
ASIN: 3764386789
EAN: 9783764386788
sales rank: 1887935

PEGylation technology and key applications are introduced by this topical volume. Basic physical and chemical properties of PEG as basis for altering/improving in vivo behaviour of PEG-conjugates such as increased stability, improved PK/PD, and decreased immunogenicity, are discussed. Furthermore, chemical and enzymatic strategies for the coupling and the conjugate characterization are reported. Following chapters describe approved and marketed PEG-proteins and PEG-oligonucleotides as well as conjugates in various stages of clinical development.

Protein Structure and Diseases, Volume 83 (Advances in Protein Chemistry)

publisher: Academic Press, published: 2011-06-10
ASIN: 0123812623
EAN: 9780123812629
sales rank: 5814999
Structural genomics is the systematic determination of 3-dimensional structures of proteins representative of the range of protein structure and function found in nature. The goal is to build a body of structural information that will predict the structure and potential function for almost any protein from knowledge of its coding sequence. This is essential information for understanding the functioning of the human proteome, the ensemble of tens of thousands of proteins specified by the human genome.

While most structural biologists pursue structures of individual proteins or protein groups, specialists in structural genomics pursue structures of proteins on a genome wide scale. This implies large scale cloning, expression and purification. One main advantage of this approach is economy of scale.

Key Features
*Examines the three dimensional structure of all proteins of a given organism, by experimental methods such as X-ray crystallography and NMR spectroscopy

* Looks at structural genomics as a foundation of drug discovery as discovering new medicines is becoming more challenging and the pharmaceutical industry is looking to new technologies to help in this mission

Protein Stability and Folding: Theory and Practice (Methods in Molecular Biology)

by: Bret A. Shirley
publisher: Humana Press, published: 1995-05-15
ASIN: 0896033015
EAN: 9780896033016
sales rank: 3149116
In Protein Stability and Folding: Theory and Practice, world-class scientists present in a single volume a comprehensive selection of hands-on recipes for all of the major techniques needed to understand the conformational stability of proteins, as well as their three-dimensional folding. The distinguished contributors provide clear, step-by-step instructions along with many troubleshooting tips, alternative procedures, and informative explanations about why certain steps are necessary. Even highly skilled researchers will find many time-saving methods. Among the techniques discussed are fluorescent, ultraviolet, and infrared spectroscopy; HPLC peptide mapping; differential scanning calorimetry; and hydrogen exchange. Shirley's Protein Stability and Folding: Theory and Practice will ensure a significant difference in the outcome of your experiments, producing the result desired even for beginners.

Handbook of Plant Lectins: Properties and Biomedical Applications

by: Els. J. M. Van Damme
publisher: Wiley, published: 1998-02-25
ASIN: 047196445X
EAN: 9780471964452
sales rank: 863471
Plant lectins are extensively used as tools and as bioactive proteins in different areas of biomedical and biological research. The Handbook of Plant Lectins provides a comprehensive yet concise overview of the biochemical properties, carbohydrate-binding specificity, biological activities and applications of most of the currently known plant lectins. This handbook consists of two major sections: an introductory guide and a quick reference dictionary. Part I acquaints the newcomer to the lectin field with the essential information on lectins and their importance to biomedicine:
* what are lectins?
* their carbohydrate-binding specificity
* effects on nutrition and immunology
* use in histochemistry
* application as therapeutic agents
Part II lists approximately 200 lectin entries in alphabetical order. Each entry deals with the lectin(s) of a particular plant and provides, (where known), details of:
* isolation and characterisation;
* sugar binding specificity;
* biological activities;
* applications;
* commercial availability; and,
* a bibliography.
Useful summary tables list lectins according to their specificity, thereby allowing the user to choose the best lectin for their application. A list of suppliers is also provided. Handbook of Plant Lectins will be of interest to biologists and biomedical researchers studying cell biology, cancer research, nutrition, immunology, pathology and physiology.

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	Cell-free Protein Synthesis: Methods and Protocols publisher: Wiley-VCH, published: 2007-12-25 ASIN: 3527316493 EAN: 9783527316496 sales rank: 2139000 With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.
	Handbook of Animal Lectins: Properties and Biomedical Applications by: David C. Kilpatrick publisher: Wiley, published: 2000-10-31 ASIN: 047189981X EAN: 9780471899815 sales rank: 876363 This comprehensive yet concise guide to animal lectins, covers all sources from unicellular protozoa and slime moulds through invertebrates to mammals and birds, and provides essential information to the widest possible readership. The last few years have seen an explosion of interest and research in animal lectins, and this book, which is divided into two parts, provides a compendium of galectins, collectins, selectins, pentraxins and other carbohydrate-binding proteins from throughout the animal kingdom. The first part introduces animal lectins on both phylogenetic and structural bases and outlines their key biomedical applications. The second, and major part, is a quick reference alphabetical directory listing around 170 lectins. Each lectin entry provides, where known, details of its: Isolation Structure Biological activities Tissue and/or subcellular distribution Possible functions Applications A bibliography for each lectin is also included and useful appendices list lectins according to their sugar specificity, characterised lectins of human origin by tissue source and commercial suppliers of animal lectins. This superb book is aimed at basic scientists and clinicians alike, and will be invaluable to both established research workers and newcomers to this area. It will be of particular interest to biochemists, cell biologists, immunologists, oncologists, pharmacologists, medical practitioners and students of medical and biological sciences.
	Modern Protein Chemistry: Practical Aspects publisher: CRC Press, published: 2001-09-12 ASIN: 0849394538 EAN: 9780849394539 sales rank: 3246068 In recent years, interest in proteins has surged. This resurgence has been driven by the expansion of the post-genomic era when structural genomics and proteomics require new techniques in protein chemistry and new applications of older techniques. Protein chemistry methods are used by nearly every discipline of biomedical research. Many techniques have been used in less traditional ways with exciting results. Modern Protein Chemistry: Practical Aspects describes the practical side of advanced techniques in protein chemistry. The book gives researchers an excellent "cost-benefit" analysis of these techniques. The contributors have been selected for their prominence in their specific fields and because they run laboratories that actively collaborate with other scientists. Researchers and practitioners, both beginners and experienced, who are looking for new ideas and who are interested in applying these more advanced methods will be assisted in their work by these commentaries. This guide provides hands-on information to complement theoretical understanding. The theory behind these methods can be found in existing books and in the original literature. However, no other guide will help you make a practical evaluation of these methods and their value to your work.
	Bioseparations of Proteins, Volume 1: Unfolding/Folding and Validations (Separation Science and Technology) by: Ajit Sadana publisher: Academic Press, published: 1997-11-17 ASIN: 0126140405 EAN: 9780126140408 sales rank: 6970109 This book covers the fundamentals of protein inactivation during bioseparation and the effect on protein processing. Bioseparation of Proteins is unique because it provides a background of the bioseparation processes, and it is the first book available to emphasize the influence of the different bioseparation processes on protein inactivation. Bioseparation of Proteins covers the extent, mechanisms of, and control of protein inactivation during these processes along with the subsequent and essential validation of these processes. The book focuses on the avoidance of protein (biologicalproduct) inactivation at each step in a bioprocess. It compares protein inactivation exhibited during the different bioseparation processes by different workers and provides a valuable framework for workers in different areas interested in bioseparations. Topics include separation and detection methods; estimates of protein inactivation and an analysis of this problem for different separation processes; strategies for avoiding inactivation; the molecular basis of surface activity and protein adsorption,process monitoring, and product validation techniques; and the economics of various bioseparation processes and quality control procedures. Key Features * Protein inactivation and other aspects of biological stability are critical to an effective bioseparation process; This book is a detailed and critical review of the available literature in an area that is essential to the effectiveness, validation, and economics of bioseparation processes for drugs and other biological products; Conveniently assembled under one cover, the survey of the literature and resulting perspective will greatly assist engineers and chemists in designingand improving their own processes; Key features of the text include: * detailed data on biological stability under various bioseparation conditions * extensive case studies from the literature on separation processes, validation, and economics * simplified analysis of protein refolding and inactivation mechanisms * consideration of adsorption theories and the effect of heterogeneity * coverage of both classical and novel bioseparation techniques, including chromatographic procedures
	Protein Synthesis: Methods and Protocols (Methods in Molecular Biology) publisher: Humana Press, published: 2010-11-10 ASIN: 1617370258 EAN: 9781617370250 sales rank: 2882724 This book presents a collection of molecular biological methods specific to protein synthesis. Chapters open with a discussion of basic background information and strategy which is then complemented by comprehensive methodological details. The book is divided into seven significant areas that cover all of the research techniques required by both experienced researchers and newcomers to the field of protein synthesis, and will prove to be an invaluable reference source on the benchtop of many protein laboratories.
	Cell Surface Receptors, Volume 68 (Advances in Protein Chemistry) by: K. Christopher Garcia Ph.D publisher: Academic Press, published: 2004-11-05 ASIN: 0120342685 EAN: 9780120342686 sales rank: 3119365 Cell Surface Receptors contains an extensive discussion of cell surface receptors in 11 chapters by experts in their field. As cell surface receptors are involved in almost every aspect of signaling throughout the body, the topic has been of high interest in the community in recent years. Selected Contents: -Structures of Axon Guidance Molecules and Their Neuronal Receptors -Shared Cytokine Signaling Receptor -NKG2D and Related Immunoreceptors -Inonotropic Glutamat Receptor Recognition and Activation -Chemotaxis Receptors and Signaling
	PEGylated Protein Drugs: Basic Science and Clinical Applications (Milestones in Drug Therapy) publisher: Birkhäuser Basel, published: 2009-10-23 ASIN: 3764386789 EAN: 9783764386788 sales rank: 1887935 PEGylation technology and key applications are introduced by this topical volume. Basic physical and chemical properties of PEG as basis for altering/improving in vivo behaviour of PEG-conjugates such as increased stability, improved PK/PD, and decreased immunogenicity, are discussed. Furthermore, chemical and enzymatic strategies for the coupling and the conjugate characterization are reported. Following chapters describe approved and marketed PEG-proteins and PEG-oligonucleotides as well as conjugates in various stages of clinical development.
	Protein Structure and Diseases, Volume 83 (Advances in Protein Chemistry) publisher: Academic Press, published: 2011-06-10 ASIN: 0123812623 EAN: 9780123812629 sales rank: 5814999 Structural genomics is the systematic determination of 3-dimensional structures of proteins representative of the range of protein structure and function found in nature. The goal is to build a body of structural information that will predict the structure and potential function for almost any protein from knowledge of its coding sequence. This is essential information for understanding the functioning of the human proteome, the ensemble of tens of thousands of proteins specified by the human genome. While most structural biologists pursue structures of individual proteins or protein groups, specialists in structural genomics pursue structures of proteins on a genome wide scale. This implies large scale cloning, expression and purification. One main advantage of this approach is economy of scale. Key Features Examines the three dimensional structure of all proteins of a given organism, by experimental methods such as X-ray crystallography and NMR spectroscopy Looks at structural genomics as a foundation of drug discovery as discovering new medicines is becoming more challenging and the pharmaceutical industry is looking to new technologies to help in this mission
	Protein Stability and Folding: Theory and Practice (Methods in Molecular Biology) by: Bret A. Shirley publisher: Humana Press, published: 1995-05-15 ASIN: 0896033015 EAN: 9780896033016 sales rank: 3149116 In Protein Stability and Folding: Theory and Practice, world-class scientists present in a single volume a comprehensive selection of hands-on recipes for all of the major techniques needed to understand the conformational stability of proteins, as well as their three-dimensional folding. The distinguished contributors provide clear, step-by-step instructions along with many troubleshooting tips, alternative procedures, and informative explanations about why certain steps are necessary. Even highly skilled researchers will find many time-saving methods. Among the techniques discussed are fluorescent, ultraviolet, and infrared spectroscopy; HPLC peptide mapping; differential scanning calorimetry; and hydrogen exchange. Shirley's Protein Stability and Folding: Theory and Practice will ensure a significant difference in the outcome of your experiments, producing the result desired even for beginners.
	Handbook of Plant Lectins: Properties and Biomedical Applications by: Els. J. M. Van Damme publisher: Wiley, published: 1998-02-25 ASIN: 047196445X EAN: 9780471964452 sales rank: 863471 Plant lectins are extensively used as tools and as bioactive proteins in different areas of biomedical and biological research. The Handbook of Plant Lectins provides a comprehensive yet concise overview of the biochemical properties, carbohydrate-binding specificity, biological activities and applications of most of the currently known plant lectins. This handbook consists of two major sections: an introductory guide and a quick reference dictionary. Part I acquaints the newcomer to the lectin field with the essential information on lectins and their importance to biomedicine: * what are lectins? * their carbohydrate-binding specificity * effects on nutrition and immunology * use in histochemistry * application as therapeutic agents Part II lists approximately 200 lectin entries in alphabetical order. Each entry deals with the lectin(s) of a particular plant and provides, (where known), details of: * isolation and characterisation; * sugar binding specificity; * biological activities; * applications; * commercial availability; and, * a bibliography. Useful summary tables list lectins according to their specificity, thereby allowing the user to choose the best lectin for their application. A list of suppliers is also provided. Handbook of Plant Lectins will be of interest to biologists and biomedical researchers studying cell biology, cancer research, nutrition, immunology, pathology and physiology.