PCR inconsistency

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Credit reward to answer the question: 0 - Question raised on 2010-07-25 23:48

I am trying to amplify 18 samples of soil DNA diluted 20x, but I am having toruble because sometimes the products would work and sometimes they wouldn't - sometimes it would be a strong amplification and sometimes mediocre. I do not know what is wrong with my technique (if any), since I always thaw all my reagents and centrifuge them, I mix the master mix, and my reagents should not be contaminated since some products do work. Furthermore, I am puzzled because I need these products to run DGGE, but when the products are verified on agarose gel, occassionally there are no results in the DGGE image itself. Any tips on improving my PCR?

total 23 hits Asked by: fuzzypeachgl1

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