CD8 enrichment from 20 spleens
Pending answer
Credit reward to answer the question: 0 -
Time remaining before closing the question: 195day3hour25min26sec
I need to enrich the CD8 population in the spleen before FACS sorting a very rare CD8 population, from a total of 20 spleens. I only have three hours to do the sorting. Since I have 20 spleens, I will need to reduce the volume of my sample and increase the concentration of CD8 in my sample. I plan to use non-CD8 beads to negatively enrich the CD8 population, before FACS sorting. My question is, there are a lot of macrophages in the spleen. Is it worth incubating the splenocytes for an hour, at 37oC in tissue-culture flasks, to remove macrophages before treating the lymphocytes with beads?
total 21 hits
Asked by:
131.111.*.*
Search ask
Related questions
Questions pending your answer