electrocompetent e faecalis

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Credit reward to answer the question: 0 - Question raised on 2011-05-02 12:18

Hi

I work with Enterococcus faecalis and I am trying to find a way of increasing the transformation efficiency. Does anyone have a protocol where the transformation efficiency for this but is at least 10^7 or better?

My second question is colony pcr. Since this bug is Gram +, colony PCR can be a bit tricky? Does anyone have a protocol that works for them?

Thanks for any help that can be offered!

HS

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