i Can see no bands in gel

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hi, i loaded the gel with pcr product and ladder but i see no bands of pcr product however i see ladder bands clearly .

what can be the problem ,i have done one mistake by setting the Tm temperature at 60 degree instead Tm 55.6 (Tm of primer) in PCR , did that created a problem or something else please let me know the reason so that i may correct myself.

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