ProteoExtract® Subcellular Proteome Extraction Kit, Mini

Assay time

Detection method

Form 10 Extractions

Format

Kit contents:
The ProteoExtract® Subcellular Proteome Extraction Kit, Mini is a tool for the differential extraction of proteins from mammalian cells according to their subcellular localization. The sequential extraction steps yield proteins in their native state, making it highly suited for demanding proteomics applications. Sample size: 3-5x10⁶ or 25-50 mg tissue.

References
Yuan, X., et al. 2002. Electrophoresis 23, 1185.Butcher, B.A., et al. 2001. J. Immunol. 167, 2193;Ott, V., et al. 2001. Pharmacogenomics J. 1, 142.Allen, L., et al. 2000. Nature 405, 819.Rabilloud, T. 2000. Anal. Chem. 72, 48A.Mejdoubi, M., et al. 1999. Biochem. Biophys. Res. Commun. 254, 93.Reymond, et al. 1997. Electrophoresis 18, 2842.Laemmli, U. K. 1970. Nature 227, 680.Lowry, O.H., et al. 1951. J. Biol. Chem. 193, 265.

Sample type Mammalian tissues and cultured cells

Sensitivity
http://www.emdchemicals.com/products/EMD_BIO-539791?WT.mc_id=EB000129