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| Nuclear run-on assay measures the transcription initiation rate, the pausing and attenuation of polymerase during transcription. |
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Nuclear run-on assay
new
protocol
PDF prootcol by Gen Matusumoto from Marimoto lab at Northwestern University.
References:
Udvardy and Selfart (1976) Eur. J. Biochem. 652:353-363.
Marzluff et al. (1974) Biochem. 13:3689-3698.
Reeder and Roeder (1972) J. Mol. Biol. 67:433-441.
Gro ...
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Last update: 05-Sep-2008 09:31 am
Showing 1 to 8 of total 94500 selected nuclear-run-on-assay articles (pdf, doc, web pages)
- nuclei isolation and nuclear run-on assay
Arabidopsis nuclei isolation and nuclear run-on (Gaudino and Pikaard, J. ... 2. Nuclear run-on reactions are for 10 minutes at 30°C in a final volume of 50 ...
- AMG Lecture 11
... be used for the Nuclear Run-on assay . Basic Strategy and Comments ... What makes the nuclear run-on assay so difficult to use, i.e., what are its limitations? ...
- week11.html
The nuclear run-on assay and the nuclease protection assay were also briefly touched upon. A nuclear run-on assay measures the transcriptional or degradational rate, ...
- Bertan's website
... studies: Nuclear Run on assay, nuclease protection assay and ... Briefly, in nuclear run on assay nucleus is isolated intact containing whole RNA ...
- Protocols
Nuclei isolation and nuclear run-on assay ... activity assay (PDF file) Promoter-dependent in vitro transcription and S1 nuclease protection assay (PDF file) ...
- PII: S0960-0760(00)00161-8
Nuclei isolation and nuclear run-on assay. Nuclei from CEM-C7 cells were isolated according to ... for nuclear run-on assay. Fig. 6 shows that the level of ...
- Herpes Simplex Virus 1 ICP27 Is Required for Transcription of Two Viral ...
Thus, we initially used a nuclear run-on. assay, which we had used previously (Godowski and ... were washed as for the nuclear run-on assay. To deter ...
- UTHSC Physiology Faculty Page for Gadiparthi N.Rao, Ph.D.
... injury, Northern blot analysis, Western blot analysis, nuclear run-on assay, ... angiogenesis, chromatin immunoprecipitation assay, RT-PCR and molecular ...
Related new papers and reviews
- [An in vitro nuclear run-on assay for nuclear function of angiogenin] Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2003 Apr;35(4):350-4
Authors: Li LY, Zhang YZ, Zheng YF, Hu GF, Xu ZP
- Nuclear run-on assay using biotin labeling, magnetic bead capture and analysis by fluorescence-based RT-PCR. Biotechniques. 2000 Nov;29(5):1012-4, 1016-7
Authors: Patrone G, Puppo F, Cusano R, Scaranari M, Ceccherini I, Puliti A, Ravazzolo R
- Fluorometric nuclear run-on assay with oligonucleotide probe immobilized on plastic plates. Anal Biochem. 1994 Aug 15;221(1):202-4
Authors: Okamoto T, Mitsuhashi M, Kikkawa Y
- Neuropeptide gene transcription in central and peripheral nervous system tissue by nuclear run-on assay. Methods Enzymol. 1989;168:681-90
Authors: White JD, LaGamma EF
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