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| Rapid amplification of cDNA end on 5' or 3' end. |
3' RACE PCR
protocol
3' RACE PCR
Protocol:
1. First strand cDNA synthesis:
- Reaction set up (total volume 20 ul)
cDNA synthesis buffer 4 ul
dNTP mixture 2 ul
oligo-dT anchor primer 1 ul
total RNA ...
5' RACE PCR
protocol
5' RACE PCR Protocol
1. First strand cDNA synthesis:
- Reaction set up:
cDNA synthesis buffer 4 ul
dNTP mixture 2 ul
gene specific primer 1 1 ul
total RNA ...
RACE amplification protocol
protocol
A detailed protocol on performing 5' and 3' RACE PCR. (Hiroyuki Takeda lab, National institute of Genetics). ...
cDNA Synthesis with Oligo dT Primers: First strand synthesis
protocol
A pdf protocol from Lahn lab at University of Chicago.
This may be done with total RNA or poly-A RNA prepped from
cell. Here one copy of cDNA will be made with a poly-T primer. (May isolate
total RNA and then prep mRNA by selection with oligoT beads to bi ...
Last update: 
06-Feb-2012 08:37 am
