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Technique / Molecular Biology / DNA-protein interactions / EMSA gel shift assay

Electrophoretic Mobility Shift Assay (EMSA) for the detection of nuclear NF-kB protocol
A protocol on EMSA detection of NF-kB with detailed description of the method. For the EMSA, nuclear extracts are incubated with a radioactive labeled oligonucleotide probe which contains the specific recognition sequence for NF-kB. (CellDeath.de) ...

A non-radioactive electrophoretic mobility shift assay for the detection of heat shock element (HSE) protocol
A non-radioactive electrophoretic mobility shift assay based on the digoxigenin detection system had been developed and applied to the analysis of the interaction between the heat shock transcription factor and the heat shock element of N. crassa. (Biochemistr ...

Introduction to the EMSA (Gel Shift) Technique new review
A tech note from Pierce on EMSA gel shift assay. It describes some critical EMSA reaction parameters: (Pierce) Target DNA Labeling and Detection Nonspecific Competitor Binding Reaction Components Electrophoresis ...

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Last update: 20-Aug-2008 12:13 pm

Showing 1 to 8 of total 355000 selected gel-shift articles (pdf, doc, web pages)

FROGS

Gel shift activities were visualized by exposing the gels to X-ray film ... gives an internal control (of sorts) for each gel shift reaction. ...

RNA probes for gel shift

RNA probes for gel shift. A. Reaction mixture. Hot Cold. 5X buffer for T3 or T7 5 10 ... Run a formamide agarose gel and blot to nitrocellulose (like a northern) to ...

Electrophoretic Mobility Shift Assay (EMSA: gel shift) for VSG ES promoter

Gel casting and running. Setup of apparatus: ... 3. Pre-run gel in 0.5x TBE for 30 min at 100V (RT: room temp) ... 8. Run gel at 100–150V for 3-4 h at 4°C. ...

Gel Shift (EMSA) Protocol

Gel Shift (EMSA) Protocol. There are multiple variations to this protocol, but we ... then load 18 ml per lane on a 5% polyacrylamide gel. ...

Gel Shift Assay

... the appropriate amount of protein for each assay diluted in 1X gel shift buffer. ... Adjust the volume to 10 µl with 1X gel shift buffer. ...

Penn MCRC > TBE OLIGO Gel shift

TBE OLIGO Gel shift. OLIGO PREPARATION. Resolubilize oligo in 100ml of 10mM ... gel ... way down gel. Transfer to Whatman paper and dry 30 minutes on gel dryer ...

Catharineís Footprint Protocol

Gel shift experiments will have given an indication of what sequences are bound ... binding buffer (as for gel-shift experiments) at appropriate concentration ...

III.C.6 GEL SUPER-SHIFT ASSAY/ EMSA Materials/Reagents: See Gel-shift assay

See Gel-shift assay. Procedure: 1. Prepare Whole ... are the same as normal gel shift: ... You may use 18 cm-height gel system and stop running after dye ...

Related new papers and reviews

Phenotyping of IL4-induced nuclear Stat6 activity in humans: quantitation after gel shift assay using immortalized cell lines.
Oncol Rep. 2003 Sep-Oct;10(5):1281-8
Authors: Zhang WJ, Thompson JL, Chorney MJ, Koltun WA

Gel shift assay of nuclear extracts from Histoplasma capsulatum demonstrates the presence of several DNA binding proteins.
Infect Immun. 2002 Apr;70(4):2238-41
Authors: Ignatov A, Keath EJ

Schistosome extracts with heat shock factor activity revealed by the gel shift assay.
Parasitology. 1994 Jan;108 ( Pt 1):35-42
Authors: Levy-Holtzman R, Schechter I

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