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Electrophoretic Mobility Shift Assay (EMSA) for the detection of nuclear NF-kB
protocol
A protocol on EMSA detection of NF-kB with detailed description of the method. For the EMSA, nuclear extracts are incubated with a radioactive labeled oligonucleotide probe which contains the specific recognition sequence for NF-kB. (CellDeath.de) ...
A non-radioactive electrophoretic mobility shift assay for the detection of heat shock element (HSE)
protocol
A non-radioactive electrophoretic mobility shift assay based on the digoxigenin detection system had been developed and applied to the analysis of the interaction between the heat shock transcription factor and the heat shock element of N. crassa. (Biochemistr ...
Introduction to the EMSA (Gel Shift) Technique
review
A tech note from Pierce on EMSA gel shift assay. It describes some critical EMSA reaction parameters: (Pierce)
Target DNA
Labeling and Detection
Nonspecific Competitor
Binding Reaction Components
Electrophoresis ...
(EMSA technique overview)
EMSA is a biochemistry method to test the specific binding of proteins to DNA fragments.
Basic steps for EMSA assay include:
1. DNA labeling with radioactivity or non-radioactivity.
2. Form Complex by mixing DNA and protein in an reaction.
3. Add nonspecific competitor in reaction to compete for the binding between DNA and protein.
4. Run native gel to separate complex with free probe.
Related protocols:
non-radioactive electrophoretic mobility shift assay based on the digoxigenin detection system
Electrophoretic Mobility Shift Assay (EMSA) (PDF)
Electrophoretic Mobility Shift Assay (EMSA) (DOC)
Electrophoretic Mobility Shift Assay (EMSA) protocol (From SpringerLink protocol, Restricted access)
EMSA protocol from Nature Methods (Nature methods, restricted access)
OpenWebWare Electrophoretic mobility shift assay EMSA protocol
Last update: 
04-May-2012 12:49 pm
