Home / Molecular Biology / DNA-protein interactions / DNase I footprinting assay
| DNase footprinting assay is used to detect DNA-protein interaction using the fact that a protein bound to DNA will often protect that DNA from enzymatic cleavage. This makes it possible to locate a protein binding site on a particular DNA molecule. |
Long Range Footprinting Protocol (SP1)
protocol
This protocol is from Machida lab at Japan National Institute of Bioscience & Human-Technology and is based on Based on Bio-Techniques,23, 300-303 (1997). ...
A modified protocol for in vivo footprinting by ligation-mediated polymerase chain reaction
protocol
A free accessible original paper on PCR footprinting.
Reference:
Nucleic Acids Res. 1994 Feb 11;22(3):532-3
A modified protocol for in vivo footprinting by ligation-mediated polymerase chain reaction.
Dimitrova D, Giacca M, Falaschi A.
I ...
DNase I Protection
protocol
A short protocol on DNase I protection from Molecular Biology of Nuclear Receptors, France.
You will have first to determine the concentration of DNaseI that cleaves
30-50% of your input DNA. A rule of thumb is to multiply this amount by 10 to get the adeq ...
Genetic Footprinting Protocol
protocol
Detailed protocols for genetic footprinting, a technique for high-resolution mapping of the functional organization of cloned genes (Singh, Crowley and Brown (1997) PNAS 94: 1304-1309).
Reference:
Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1304-9.
Hig ...
Footprinting
review
Footprinting is a method for determining the exact DNA sequence to which a particular DNA-binding protein binds. This brief introduction is from Kimball's Biology Pages. This page also contains a nice illustration on principle of footprinting. ...
More DNase I footprinting assay protocols
(Molecular Biology of the Cell)
II. Basic Genetic Mechanisms 7. Control of Gene Expression DNA-Binding Motifs in Gene Regulatory Proteins
(Molecular Cell Biology)
10. Regulation of Transcription Initiation 10.2. Bacterial Transcription Initiation
Last update: 
16-Jul-2010 10:47 am
