Molecular Biology / DNA analysis techniques / Comet assay protocols and methods

The alkaline (pH>13) SCG comet assay protocol
A detailed protocol by Vasquez and Tice (Integrated Laboratory Systems). PROTOCOL FOR THE APPLICATION OF THE pH>13 ALKALINE SINGLE CELL GEL (SCG) ASSAY TO THE DETECTION OF DNA DAMAGE IN MAMMALIAN CELLS The general principle of comet assay: The comet ass ...

Short protocol of comet assay protocol
A very brief comet assay protocol by S. Nehls. ...

Natural comet assay protocol
A free accessible pdf original paper on natural comet assay technique. Reference: INT. J. RADIAT. BIOL., 1993,VOL. 64,NO. 4, 349-358 Detection of DNA double-strand breaks through the cell cycle after exposure to X-rays, bleomycin, etoposide and ...

Comet assay with DNA repair enzymes protocol
Protocol of comet assay modified for detection of oxidised bases with the use of bacterial repair endonucleases. (MS doc file, Comet Assay Interest Group) ...

Collection of comet assay protocols protocol
A collection of comet assay and apoptosis protocols from Comet Assay Forum. COMET ASSAY PROTOCOL PROTOCOL FOR OXIDATIVE DNA DAMAGE APOPTOSIS PROTOCOL PROTOCOL FOR DNA STRAND BREAKS SCHEME FOR VISUAL SCORING ...

Comet assay forum recommended site
A public comet assay interest group forum. It features various resources and protocols on comet assay. Comet assay measures, double strand breaks (DSBs), single strand breaks (SSBs), alkali labile sites, oxidative DNA base damage, DNA-DNA/DNA-protein/DNA-Drug ...

Comet assay interest group site
Single cell gel (SCG) electrophoresis or 'Comet assay' is a rapid and very sensitive fluorescent microscopic method to examine DNA damage and repair at individual cell level. This assay has critical applications in fields of toxicology ranging from aging and ...

The comet assay: a method to measure DNA damage in individual cells
(Nat Protoc. 2006;1(1):23-9.)
Olive PL, Banáth JP.

British Columbia Cancer Research Center, 675 W. 10th Avenue, Vancouver, British
Columbia V5Z 1L3, Canada. polive@bccrc.ca

We present a procedure for the comet assay, a gel electrophoresis-based method
that can be used to measure DNA damage in individual eukaryotic cells. It is
versatile, relatively simple to perform and sensitive. Although most
investigations make use of its ability to measure DNA single-strand breaks,
modifications to the method allow detection of DNA double-strand breaks,
cross-links, base damage and apoptotic nuclei. The limit of sensitivity is
approximately 50 strand breaks per diploid mammalian cell. DNA damage and its
repair in single-cell suspensions prepared from yeast, protozoa, plants,
invertebrates and mammals can also be studied using this assay. Originally
developed to measure variation in DNA damage and repair capacity within a
population of mammalian cells, applications of the comet assay now range from
human and sentinel animal biomonitoring (e.g., DNA damage in earthworms crawling
through toxic waste sites) to measurement of DNA damage in specific genomic
sequences. This protocol can be completed in fewer than 24 h.

Technique / Molecular Biology / DNA analysis techniques / Comet assay