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Technique / Immunology / Antibody / epitope mapping
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B cell epitope mapping using synthetic peptides. (Curr Protoc Immunol. 2004 May;Chapter 9:Unit 9.4.) Carter JM, Loomis-Price L. Palo Alto, California, USA. Synthetic peptides are used for identification of functional B cell epitopes in antibody preparations. ELISA-type assays are used to identify sequences of proteins comprising antibody-binding regions. This unit describes three peptide display formats. Pepscan (pins) and SPOTs (cellulose membranes) may be used as solid-phase support media for peptide synthesis, followed by ELISA directly on the resulting peptide array. Alternatively, peptides may be cleaved from the array and tested in a standard microplate-based antibody capture ELISA format. The discussion includes choosing the peptide sequences by length and overlap, as well as determination of the minimum essential sequence for antibody binding. This method is highly effective for continuous epitopes and is often also useful for discontinuous epitopes. 
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Bioscience news papers
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Last update: 
19-Aug-2008 11:06 am
- Epitope mapping of a monoclonal antibody against human thrombin by H ... Epitope mapping is the process of identification of the mo ... mapping to epitope regions that contain cleavage sites and ... well for epitope mapping because ...
- Monoclonal Antibody Epitope Mapping Describes Tailspike -Helix Folding ... Combined with the epitope mapping, these results indi- cate portions of the ... Indeed, careful epitope mapping experiments and anti ...
- Epitope mapping factor VIII A2 domain by affinity-directed mass ... Epitope mapping factor VIII A2 domain by affinity-directed mass spectrometry: residues 497-510 ... antibodies from patient plasmas, mapping epitopes have ...
- Linear IgE epitope mapping of the English walnut (Juglans regia) major ... Key words: Epitope mapping, 2S albumin, walnut, nut allergy, Jug. r 1, food allergy ... Epitope mapping of the cat (Felis domesticus) ...
- Mapping the Protein Surface of Human Immunodeficiency Virus Type 1 ... libraries provide a rapid alternative for surface epitope mapping. Diverse ... Keywords: human antibody repertoires; epitope mapping; HIV infection; ...
- Mapping protein–protein interactions by affinity-directed mass spectrometry ... antibody (epitope-containing ... Epitope Mapping Using the Phage Display Library. It is of ... spectrometry for epitope mapping to three other antibodies ...
- A new method for mapping discontinuous antibody epitopes to reveal ... ... linear sequence, so the epitope mapping ... Antibody Epitope Mapping ... Figure 2: Mapping of the anti-actin antibody epitope VPHPTWMR onto the surface ...
- Characterization of monoclonal antibody epitope specificity using ... Epitope mapping using monoclonal antibodies (MAbs) is a powerful tool in ... epitope mapping techniques: the reason for. the negative response (lack of antigen ...
- Epitope mapping of allergen ovalbumin using biofunctionalized magnetic beads packed in microfluidic channels The first step towards epitope-based vaccines. J Chromatogr A. 2008 Jul 26;
Authors: Jankovicova B, Rosnerova S, Slovakova M, Zverinova Z, Hubalek M, Hernychova L, Rehulka P, Viovy JL, Bilkova Z
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