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Long PCR Protocol
protocol
The key to LA PCR is an enzyme, TaKaRa LA TaqTM. It is a thermostable DNA polymerase, which possesses 3กฏ to 5กฏ exonuclease activity, or proofreading activity. The efficiency drastically declines when incorrect bases are incorporated. The 3กฏ to 5กฏ exonuclea ...
Long PCR Reagents and Guidelines
protocol
From Church lab at Harvard University.
Efficient Long PCR results from the use of two polymerases: a non-proofreading polymerase
is the main polymerase in the reaction, and a proofreading polymerase (3' to 5' exo) is present at a lower concentration. Foll ...
PCR fidelity of pfu DNA polymerase and other thermostable DNA polymerases
review
An open access original paper on fidelity of different DNA polymerase used in high fidelity PCR.
Nucleic Acids Res. 1996 Sep 15;24(18):3546-51.
PCR fidelity of pfu DNA polymerase and other thermostable DNA polymerases.
Cline J, Braman JC, Hogrefe HH.
...
Long PCR of circular mitochondria DNA
troubleshooting
I need a protocol for PCR of circular DNA. I would like to amplifie the whole
mtDNA (16-17kb) of mytilus by using a long PCR method for circular NA. ... ...
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Troubleshooting for Long Template PCR
troubleshooting
It describes various problems that may occur during amplification of long DNA sequences and recommendations for avoiding them. Troubleshooting from Roche Applied Bioscience PCR manual. ...
More high fidelity pcr protocols
Last update: 
10-Feb-2012 01:22 am
