Home / Molecular Biology / DNA analysis techniques / DNA ligation
PCR ligation protocol
review
Ligation of PCR products
Materials:
• Invitrogen TA cloning kit
• LB Liquid Medium (500 ml) : Autoclave, store at 4 C.
Tryptone 5 g
Yeast extract 2.5 g
NaCl 5 g
• LB Agar Plate (500 ml) : Autoclave, store at 4 C.
Tryptone 5 g
Yeast extract ...
DNA ligation protocol
review
Low melting agarose DNA ligation protocol
2x Bacteriophage T4 DNA ligase mixture
Restriction endonucleases
Protocol:
- digest target DNA in EP tube (~250 ng of fragment in 20ul volume).
- run low-melting-temperature agarose gel to separate the dig ...
DNA ligation overview
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DNA ligation is the key step for successful DNA cloning experiment. It involves linking two or more DNA fragments together using T4 DNA ligase. DNA ligation requires DNA fragments (with compatible cohesive or blund ends), reaction buffer with ATP, and T4 DNA ligase.
()
DNA ligation is the key step for successful DNA cloning experiment. It involves linking two or more DNA fragments together using T4 DNA ligase. DNA ligation requires DNA fragments (with compatible cohesive or blund ends), reaction buffer with ATP, and T4 DNA ligase.
Last update: 
04-Feb-2012 03:54 am
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