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Technique / Molecular Biology / PCR / Touchdown PCR
Touchdown PCR technique was developed by Don et al in 1991. The initial cycles of touchdown PCR has annealing temperature higher than the Tm of primer pair and decrease gradually until it reaches Tm. The touchdown PCR allows early phase accumulation of more specific products which then serve as templates for later phase PCR amplification thus can decrease the overall non-specific amplification.

Original paper: 'Touchdown' PCR to circumvent spurious priming during gene amplification new protocol
This is the original paper on touchdown PCR technique by Don et al on Nucleic Acids Res. 1991 July 25; 19(14): 4008. A frequently encountered problem in PCR amplification of target gene sequences, especially from complex genomes, is the appearance of spuri ...

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Last update: 04-Sep-2008 02:39 am

Showing 1 to 8 of total 3400 selected touchdown-pcr articles (pdf, doc, web pages)

Wheat Applied Genomics - Leaf rust resistance genes Lr21 and Lr25

... linked to these genes and can be used simultaneously by multiplex touchdown PCR ... Touchdown PCR for detecting Lr29 and Lr25. ...

Ac Protocols

1. iPCR-1 (standard inverse PCR) 2. iPCR-2 (touchdown inverse PCR) ... First round touchdown PCR protocol: 1 cycle: T=96 °C for 3'00" 15 cycles: T=94 °C for 0'45" ...

Long PCR Protocol

When long PCR amplification is required, a significant increase in ... (2) Autosegment extension (Touchdown PCR) Cycles. Temperature. Time. 1. 94°C. 1 min. 14 ...

Lecture 17 PCR Technology

polymerase provides a highly efficient method to clone PCR ... Touchdown PCR. When the melting temperature of hybrids between primers ...

Microsatellite multiplex panels for genetic studies of three species

touchdown' PCR. protocols (I and II) were evaluated for each of the 14. panels. ... and touchdown PCR to screen murine microsatellite polymorph- isms. ...

Evidence for an Uncommon Microsatellite Instability on Mouse ...

Figure 1. "Multiplex Touchdown-PCR" products of D4MIT316 (112 bp), D2MIT126 (160 ... touchdown annealing of this MT-PCR is 60. Huntsville, AL (April, 1995 ...

HotStarTaq PCR Handbook

touchdown PCR (see appendix, page 29). 10. Incorrect ... Touchdown PCR ... your thermal cycler for touchdown PCR, you should refer to the ...

Microsoft Word - PROTOCOL DNA Seq - Primers & PCR Protocols

Touchdown PCR: Initial annealing temperature in cycle 1 (e.g., 58ºC), with a ... Touchdown' PCR to circumvent spurious priming during gene amplification. ...

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