biowww.net
Home /Forums /Molecular /Cell /Genetics /Proteomics /Neuroscience /Immunology /Bioinformatics /Histology /Pharmacology /Jobs /Books /Blog /Methods
Search biowww:

Technique / Molecular Biology / PCR / GC-rich PCR


Protocol for Enhancing PCR of Very Difficult Regions protocol
DNA templates with high GC content and short repeats sometimes are very difficult for PCR amplification. This PCR protocol for overcoming difficult DNA G/C rich regions uses Polyoxyethylenesorbitan monolaurate and Igepal CA-630. (Ziyun Yao, Shaoping Lin, HongM ...

PCR across a highly GC rich tandem repeat region troubleshooting
I am trying to PCR across a tandem repeat region which is highly GC rich (approx 82%). The repeat region is asymmetrical in that one strand contains mostly Cs and the opposite strand mostly Gs. The repeat unit varies in size from 2.5-5Kb with most of ...



(GC-rich PCR technique papers)
1: Hube F, Reverdiau P, Iochmann S, Gruel Y.
Improved PCR method for amplification of GC-rich DNA sequences.
Mol Biotechnol. 2005 Sep;31(1):81-4. Review.

2: Bachmann HS, Siffert W, Frey UH.
Successful amplification of extremely GC-rich promoter regions using a novel
'slowdown PCR' technique.
Pharmacogenetics. 2003 Dec;13(12):759-66.

3: Moreau A, Wang DS, Forget S, Duez C, Dusart J.
GC-rich template amplification by inverse PCR. DNA polymerase and solvent
effects.
Methods Mol Biol. 2002;192:75-80.

4: Le Cam L, Polanowska J, Fajas L, Fabbrizio E, Sardet C.
Improved LM-PCR procedure for in vivo footprinting analysis of GC-rich
promoters.
Biotechniques. 1999 May;26(5):840-3.

5: Henke W, Herdel K, Jung K, Schnorr D, Loening SA.
Betaine improves the PCR amplification of GC-rich DNA sequences.
Nucleic Acids Res. 1997 Oct 1;25(19):3957-8.

6: Moreau A, Duez C, Dusart J.
GC-rich template amplification by inverse PCR. DNA polymerase and solvent
effects.
Methods Mol Biol. 1997;67:47-53.

7: Turner SL, Jenkins FJ.
Use of deoxyinosine in PCR to improve amplification of GC-rich DNA.
Biotechniques. 1995 Jul;19(1):48-52.

8: Moreau A, Duez C, Dusart J.
Improvement of GC-rich template amplification by inverse PCR.
Biotechniques. 1994 Aug;17(2):232-4. Erratum in:
Biotechniques 1994 Oct;17(4):684.

9: Agarwal RK, Perl A.
PCR amplification of highly GC-rich DNA template after denaturation by NaOH.
Nucleic Acids Res. 1993 Nov 11;21(22):5283-4.

10: Sun Y, Hegamyer G, Colburn NH.
PCR-direct sequencing of a GC-rich region by inclusion of 10% DMSO: application
to mouse c-jun.
Biotechniques. 1993 Sep;15(3):372-4.

11: Sheffield VC, Beck JS, Stone EM, Myers RM.
A simple and efficient method for attachment of a 40-base pair, GC-rich
sequence to PCR-amplified DNA.
Biotechniques. 1992 Mar;12(3):386-8.


    Last update: RSS feeds 29-Aug-2008 07:49 pm

Showing 1 to 8 of total 11500 selected gc-rich-pcr,-difficult-pcr articles (pdf, doc, web pages)
Related new papers and reviews