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Technique / Molecular Biology / DNA microarray gene array / mRNA amplification for microarray analysis
| High-throuput microarray analysis requires large quantity of RNA. To address this issue, high-fidelity mRNA amplification technique for gene profiling was developed in 2000 by Wang E et al (Nature Biotechnology, 2000 Apr;18(4):457-9). |
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Bioscience news papers
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Last update: 
30-Aug-2008 03:45 am
- Protocol of mRNA amplification Protocol for mRNA amplification and target preparation ... 37C for 5min to digest mRNA, 94C for 2min to denature, 65C for 1min for specific ...
- Protocol of mRNA amplification Protocol for mRNA amplification (Wang) Wang et. al., Nature Biotechnology, April, ... Protocol for mRNA amplification. aRNA purification using Qiagen RNeasy ...
- Protocol for mRNA Amplification and Target Preparation RNA amplification is of interest to a broad range of researchers in genomics. ... 37 ºC for 5 min. to digest mRNA, 94 ºC for 2 min. to denature, 65 ºC for 1 ...
- in vitro from amplified mRNA, we have analyzed in vitro transcription. amplification products. ... competing side reactions, mRNA amplification by cDNA ...
- Correlation of amplification and overexpression of the c-myc oncogene ... of its amplification, mRNA and protein overexpression in breast ... For each analysis of gene copy amplification (FISH), mRNA ...
- Insights Into the Relation Between mRNA and Protein Expression Patterns ... observed change in mRNA amplification factor for the entire. system. ... A plot of the relative mRNA and protein amplification factors for a change in ...
- BMC Genomics ear Amplification Method for Analyzing the mRNA Popula ... fidelity mRNA amplification for gene profiling Nat Biotechnol. 2000, 18:457-9. 16. ...
- Rapid Amplification of mRNA Ends By PCR (RACE) Rapid Amplification of mRNA Ends By PCR (RACE) Buffers. 10X PCR buffer. 100 mM Tris.Cl (pH 8.3) ... 3'-end Amplification of mRNA by the PCR ...
- [Thyroglobulin mRNA amplification in peripheral blood of patients with differentiated thyroid carcinoma: what does it really mean?] Arq Bras Endocrinol Metabol. 2006 Jun;50(3):427-35
Authors: Coelho SM, Vaisman M, de Carvalho DP - Biologically relevant effects of mRNA amplification on gene expression profiles. BMC Bioinformatics. 2006;7:200
Authors: van Haaften RI, Schroen B, Janssen BJ, van Erk A, Debets JJ, Smeets HJ, Smits JF, van den Wijngaard A, Pinto YM, Evelo CT - Limitations of mRNA amplification from small-size cell samples. BMC Genomics. 2005;6:147
Authors: Nygaard V, Holden M, Løland A, Langaas M, Myklebost O, Hovig E - Activation of PI3K/Akt pathway by PTEN reduction and PIK3CA mRNA amplification contributes to cisplatin resistance in an ovarian cancer cell line. Gynecol Oncol. 2005 Apr;97(1):26-34
Authors: Lee S, Choi EJ, Jin C, Kim DH - Reproducibility, fidelity, and discriminant validity of mRNA amplification for microarray analysis from primary hematopoietic cells. J Mol Diagn. 2005 Feb;7(1):48-56
Authors: Li L, Roden J, Shapiro BE, Wold BJ, Bhatia S, Forman SJ, Bhatia R - Design of a real time quantitative PCR assay to assess global mRNA amplification of small size specimens for microarray hybridisation. J Clin Pathol. 2004 Dec;57(12):1278-87
Authors: Choesmel V, Foucault F, Thiery JP, Blin N - Linear mRNA amplification from as little as 5 ng total RNA for global gene expression analysis. Biotechniques. 2004 Nov;37(5):854-7
Authors: Dafforn A, Chen P, Deng G, Herrler M, Iglehart D, Koritala S, Lato S, Pillarisetty S, Purohit R, Wang M, Wang S, Kurn N - An mRNA amplification procedure with directional cDNA cloning and strand-specific cRNA synthesis for comprehensive gene expression analysis. Genomics. 2004 Oct;84(4):715-29
Authors: Ohtsuka S, Iwase K, Kato M, Seki N, Shimizu-Yabe A, Miyauchi O, Sakao E, Kanazawa M, Yamamoto S, Kohno Y, Takiguchi M
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