Home / Proteomics and protein biochemistry / Protein-protein interactions / Fluorescence resonance energy transfer FRET
| FRET is an important tool to study protein-protein interactions, protein-DNA interactions, protein conformational changes and other molecular dynamics quantification. Mesh term: A type of FLUORESCENCE SPECTROSCOPY using two FLUORESCENT DYES with overlapping emission and absorption spectra, which is used to indicate proximity of labeled molecules. This technique is useful for studying interactions of molecules and PROTEIN FOLDING. |
Interaction mating methods in two-hybrid systems
protocol
Yeast two hybrid interaction mating methods. PDF version of the reprint from Finley lab at Wayne State University.
Reference:
Methods in Enzymology, Applications of Chimeric Genes and Hybrid Proteins, Part C, Protein-Protein Interactions and Genomics, ed ...
Fluorescence Resonance Energy Transfer (FRET)
review
FRET is an elegant experimental tool to examine the spatial relationship between two proteins in vivo. (University of Washington, Dept. Biochemistry)
This page is the first in a series of linked web pages that describe the approach. The pages include:
In ...
Introduction on fluorescence resonance energy transfer (FRET)
review
An brief introduction on fluorescence resonance energy transfer (FRET) with illustrations. (FuN in life, University of Ulm, Germany)
Fluorescence resonance energy transfer (FRET) is a nonradiative process by which the excitation energy can be passed from a ...
More Fluorescence resonance energy transfer protocols
Last update: 
30-Jan-2012 05:03 am
