Cell Biology / Cell culture / Cell culture contamination protocols and methods

Mycoplasma Detection Using DNA Staining protocol
A PDF Protocol for mycoplasma detection using DNA staining. It includes images of the staining. This is an alternative way of detection mycoplasma contamination in cell culture. (Corning Inc.) ...

Understanding and Managing Cell Culture Contamination new recommended review
A very detailed PDF technique bulletin written by John Ryan, Ph.D., Corning Incorporated. It addresses some common contamination problems in cell culture and solutions to control and eliminate the cell culture contamination. Content: Introduction ...

Mycoplasma Detection and Elimination new review
Mycoplasma contamination is not without serious consequences as virtually every cellular process can be altered by the infection. In the worst scenario, contamination leads to diminished cell growth and eventually to the loss of cultures. (DSMZ, German Collect ...

Index of Typical Cell Culture Contaminants new recommended troubleshooting
This is a trouble-shooting guide for cell culture contamination. If you have a contamination problem, select what you believe to be the organism from the short descriptions in this page and click on the image to go to a more detailed page. This troubleshoot ...

Fungi contamination in cell culture work troubleshooting
Fungi contamination is commonly seen in cell culture work. Here is an old discussion thread from bio newsgroup on fungi contamination and use of antibiotics. We have comme across several cultivations of mammalian cells being "infected" by fibric material. T ...

Detection of Mycoplasma by Culture Protocol recommended media
A detailed protocol for detection of mycoplasma in cell culture from Sigma Aldrich's "Fundamental Techniques in Cell Culture, A laboratory Handbook". This is a free tech book. (Sigma Aldrich) ...

Assessing and controlling microbial contamination in cell cultures.
(Curr Protoc Cell Biol. 2001 May;Chapter 1:Unit 1.5.)
Coté R.

Becton Dickinson Microbiology Systems, Sparks, Maryland, USA.

This unit describes how to detect and recognize bacterial, fungal, or mycoplasma
contamination and how to verify the presence of mycoplasma. Strategies are
described for dealing with culture contamination, if necessary.

(Selected mycoplasma detection technique papers)
1: Bruchmuller I, Pirkl E, Herrmann R, Stoermer M, Eichler H, Kluter H, Bugert
P.
Introduction of a validation concept for a PCR-based Mycoplasma detection
assay.
Cytotherapy. 2006;8(1):62-9.

2: Angulo AF, Jacobs MV, van Damme EH, Akkermans AM, de Kruijff-Kroesen I,
Brugman J.
Colistin sulfate as a suitable substitute of thallium acetate in culture media
intended for mycoplasma detection and culture.
Biologicals. 2003 Sep;31(3):161-3.

3: Garner CM, Hubbold LM, Chakraborti PR.
Mycoplasma detection in cell cultures: a comparison of four methods.
Br J Biomed Sci. 2000;57(4):295-301.

4: Hopert A, Uphoff CC, Wirth M, Hauser H, Drexler HG.
Mycoplasma detection by PCR analysis.
In Vitro Cell Dev Biol Anim. 1993 Oct;29A(10):819-21.

5: Uphoff CC, Brauer S, Grunicke D, Gignac SM, MacLeod RA, Quentmeier H, Steube
K, Tummler M, Voges M, Wagner B, et al.
Sensitivity and specificity of five different mycoplasma detection assays.
Leukemia. 1992 Apr;6(4):335-41.

6: Freiberg EF, Masover GK.
Mycoplasma detection in cell culture by concomitant use of bisbenzamide and
fluoresceinated antibody.
In Vitro Cell Dev Biol. 1990 Jun;26(6):585-8.

(Selected review on cell culture contamination and prevention)
1: Langdon SP.
Cell culture contamination: an overview.
Methods Mol Med. 2004;88:309-17.

2: Lincoln CK, Gabridge MG.
Cell culture contamination: sources, consequences, prevention, and elimination.
Methods Cell Biol. 1998;57:49-65.

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