Technique / Cell Biology / Cell culture / Cell preservation

Cell freezing protocol for cells growing in log phase new protocol
Cell freezing protocol: Exponentially growing cells (log phase) can be stored frozen for many years using cryoprotective agent such as DMSO and glycerol. 1. Trypsinize subconfluent cells with 1x Trypsin medium for few minutes, watch cells round off and d ...

Thawing freezing vial of cells protocol
Cell thawing protocol from Morimoto lab at Northwestern University. (PDF file) ...

Trypan blue staining of cell culture protocol
Trypan blue staining for cell culture from Morimoto lab at Northwestern University. (PDF file) ...

Freezing Cells troubleshooting
Does any of you have any experience in freezing (and storing) single cells at -20 dgr.C ? What happens if a cell suspension (with DMSO) goes trough a temperature profile of -10 to -70 degr.C? Do you know of any references ... ... Click followi ...

Non-enzymatic Methods for Cell Harvesting troubleshooting
Nalge Nunc International. In some cases it is necessary to harvest cells culture vessels without using protein digesting enzymes, proteases, such as trypsin, collagenase and pronase. These proteases may modify cellular proteins or cell membranes, thus af ...

Aspects of Cryo Preservation troubleshooting
Nalge Nunc International. A variety of types and brands of vials are used for the storage of biological materials at low temperature. The purpose of storage at low temperature is to keep and maintain materials for later use, e.g. to preserve original ...

Cryopreservation Application Guide troubleshooting
The Cryopreservation Manual (pdf), A Guide to Cryopreservation Techniques is a practical handbook for scientists who are responsible for an organization’s cryopreservation program. The cryopreservation manual is written by: Kelvin G. M. Brockbank, PhD Jam ...

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