Technique / Cell Biology / Cell culture / Common cell line culture

Protocol For Spliting/Plating NIH-3T3 Cells protocol
Anita¡¯s brief Protocol For Spliting/Plating NIH-3T3 Cells from Meyer lab at Stanfrod University. (PDF file) ...

Feeder Layer Tissue Culture protocol
A feeder layer tissue culture protocol using 3T3 cells as feeder layer. (Shay/Wrigth Laboratory, SouthWestern Medical Center) ...

293 cells in serum-free medium? troubleshooting
Does anybody know if 293 cells (Human Embryonic Kidney) tolerate serum-free or reduced serum culture medium, and for how long? I'm hoping to purify a protein secreted from a stable 293 line, and the less serum I can get away with, the easier it will b ...

Establishing stable cell lines... troubleshooting
I am trying to establish stable mammalian cell lines expressing a transfected gene by G418 selection in CHO cells. I treat the transfected cells with G418 @ 400ug/ml (I assume this is this enough ?) but ... ... ...

Questions on creating stable transfectants troubleshooting
I have come round to doing my first stable transfection experiment and have the following questions and wonder if anyone can help: 1) Can anyone provide any reference for the theory of stable transfection? I only know that the DNA goes into the ...

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