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Protocol For Spliting/Plating NIH-3T3 Cells
protocol
Anita¡¯s brief Protocol For Spliting/Plating NIH-3T3 Cells from Meyer lab at Stanfrod University. (PDF file) ...
Feeder Layer Tissue Culture
protocol
A feeder layer tissue culture protocol using 3T3 cells as feeder layer. (Shay/Wrigth Laboratory, SouthWestern Medical Center) ...
293 cells in serum-free medium?
troubleshooting
Does anybody know if 293 cells (Human Embryonic Kidney) tolerate
serum-free or reduced serum culture medium, and for how long? I'm hoping
to purify a protein secreted from a stable 293 line, and the less serum I
can get away with, the easier it will b ...
Establishing stable cell lines...
troubleshooting
I am trying to establish stable mammalian cell lines
expressing a transfected gene by G418 selection in CHO
cells.
I treat the transfected cells with G418 @ 400ug/ml (I
assume this is this enough ?) but ... ...
...
Questions on creating stable transfectants
troubleshooting
I have come round to doing my first stable transfection experiment and have
the following questions and wonder if anyone can help:
1) Can anyone provide any reference for the theory of stable transfection? I
only know that the DNA goes into the ...
Last update: 
09-Feb-2012 04:34 am
