Technique / Cell Biology / Cell culture

Cell culture is the process by which prokaryotic, eukaryotic or plant cells are grown under controlled conditions. In practice the term "cell culture" has come to refer to the culturing of cells derived from multicellular eukaryotes, especially animal cells. The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture.

Basic techniques in mammalian cell tissue culture.
(Curr Protoc Cell Biol. 2007 Sep;Chapter 1:Unit 1.1)
Phelan MC.
Molecular Pathology Laboratory Network, Maryville, Tennessee, USA.

Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells.

(Selected reviews on cell culture techniques)
1: Seth G, Hossler P, Yee JC, Hu WS.
Engineering cells for cell culture bioprocessing--physiological fundamentals.
Adv Biochem Eng Biotechnol. 2006;101:119-64.

2: van Noort JM.
Human glial cell culture models of inflammation in the central nervous system.
Drug Discov Today. 2006 Jan;11(1-2):74-80.

3: Seth P, Major EO.
Human brain derived cell culture models of HIV-1 infection.
Neurotox Res. 2005 Oct;8(1-2):83-9.

4: Iwaguro H, Asahara T.
Endothelial progenitor cell culture and gene transfer.
Methods Mol Med. 2005;112:239-47.

5: Dimri G, Band H, Band V.
Mammary epithelial cell transformation: insights from cell culture and mouse
models.
Breast Cancer Res. 2005;7(4):171-9. Epub 2005 Jun 3.

6: Forbes B, Ehrhardt C.
Human respiratory epithelial cell culture for drug delivery applications.
Eur J Pharm Biopharm. 2005 Jul;60(2):193-205. Epub 2005 Apr 21.

7: Gudjonsson T, Villadsen R, Ronnov-Jessen L, Petersen OW.
Immortalization protocols used in cell culture models of human breast
morphogenesis.
Cell Mol Life Sci. 2004 Oct;61(19-20):2523-34.

8: Ishikawa M, Asahara T.
Endothelial progenitor cell culture for vascular regeneration.
Stem Cells Dev. 2004 Aug;13(4):344-9.

9: Arden N, Betenbaugh MJ.
Life and death in mammalian cell culture: strategies for apoptosis inhibition.
Trends Biotechnol. 2004 Apr;22(4):174-80.

10: MacLeod KG, Langdon SP.
Essential techniques of cancer cell culture.
Methods Mol Med. 2004;88:17-29.

11: Langdon SP.
Basic principles of cancer cell culture.
Methods Mol Med. 2004;88:3-15.

12: Korke R, Rink A, Seow TK, Chung MC, Beattie CW, Hu WS.
Genomic and proteomic perspectives in cell culture engineering.
J Biotechnol. 2002 Mar 14;94(1):73-92.

13: Stacey G, Viviani B.
Cell culture models for neurotoxicology.
Cell Biol Toxicol. 2001;17(4-5):319-34.

14: Sambruy Y, Ferruzza S, Ranaldi G, De Angelis I.
Intestinal cell culture models: applications in toxicology and pharmacology.
Cell Biol Toxicol. 2001;17(4-5):301-17.

15: Battle T, Stacey G.
Cell culture models for hepatotoxicology.
Cell Biol Toxicol. 2001;17(4-5):287-99.

16: Laken HA, Leonard MW.
Understanding and modulating apoptosis in industrial cell culture.
Curr Opin Biotechnol. 2001 Apr;12(2):175-9.

17: Crane MS.
Mutagensis and cell transformation in cell culture.
Methods Cell Sci. 1999;21(4):245-53.

18: Lubiniecki AS.
Elimination of serum from cell culture medium.
Dev Biol Stand. 1999;99:153-6.

19: Helmrich A, Barnes D.
Animal cell culture equipment and techniques.
Methods Cell Biol. 1998;57:3-17.

20: al-Rubeai M, Singh RP.
Apoptosis in cell culture.
Curr Opin Biotechnol. 1998 Apr;9(2):152-6.

21: Ropke C.
Thymic epithelial cell culture.
Microsc Res Tech. 1997 Aug 1;38(3):276-86.

22: Hu WS, Aunins JG.
Large-scale mammalian cell culture.
Curr Opin Biotechnol. 1997 Apr;8(2):148-53.

23: Pollard JW.
Basic cell culture.
Methods Mol Biol. 1997;75:1-11.

24: Reiter M, Bluml G.
Large-scale mammalian cell culture.
Curr Opin Biotechnol. 1994 Feb;5(2):175-9.

cell culture contamination help
(cell culture forum)
help on tissue culture contamination problem

Books on cell and tissue culture methods
(Biowww bookshelf)
Cell and tissue culture techniques

Figure. Stages in the establishment of a cell culture
(Molecular Cell Biology)
6. Manipulating Cells and Viruses in Culture 6.2. Growth of Animal Cells in Culture

Table. Composition of a Typical Medium Suitable for the Cultivation of Mammalian Cells
(Molecular Biology of the Cell )
III. Methods 8. Manipulating Proteins, DNA, and RNA Isolating Cells and Growing Them in Culture

Table. Some Commonly Used Cell Lines
(Molecular Biology of the Cell )
III. Methods 8. Manipulating Proteins, DNA, and RNA Isolating Cells and Growing Them in Culture

Silicon 3t3 culture
(Cell biology forum)
3t3 mouse fibroblast culture

EGF culture supplement question
(Cell biology forum)
epithelial cell culture media need to be supplemented by EGF

HUVEC cell culture
(Cell biology forum)
specialized endothelial enrichment media

HepG2 contamination help
(cell biology forum)
previously stored stable cell lines of HepG2 cells

Cycloheximide: positive control
(cell biology forum)
blocking protein synthesis

Cell culture contamination
(Forum)
tissue culture contamination discussion

culture condition for CHO cell line
(Forum)
CHO cell culture condition

Cell culture and cytokine
(Forum)
Cell culture troubleshooting

How to culture stable cell line?
(Forum)
Stable cell line culture