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Cell culture contamination (6)

Cell culture medium (2)

Cell preservation (7)

Common cell line culture (5)

Drosophila cell culture (3)

ES stem cell culture (6)

Insect cell culture (1)

Plant cell culture (1)

Primary cell culture (4)

Suspension cell culture (1)


Cell counting using a hemacytometer protocol
A detailed procedure on how to count cells using hemacytometer. (3 pages of PDF file, from Marimoto lab at Northwestern University). ...

Protocol for Cleaning and Calibrating Incubator protocol
A PDF Protocol for Cleaning and Calibrating Incubator from Meyer lab at Stanford University. ...

Home-made Low Oxygen Tissue Culture Chambers protocol
Shay/Wright Lab Low Oxygen Tissue Culture Chambers. (PDF file) Cheap, provides an easy way to devote part of an incubator to low oxygen conditions. Modular, meaning there is no cross-contamination between culture infections in the incubator, and if the CO2 ...

General cell culture protocol new protocol
Cell culture protocol introduction: 1. Initiate cell culture from frozen cells: - Warm up appropriate cell culture media for the cell line in 37c waterbath for at least 30 min. - Take screw-cap vial containing cells in frozen medium from nitrogen tank and ...

Coverslip preparations for cell culture new protocol
Protocol for preparation of coverslips for better cell adhesion during cell culture. This coverslip cleaning and coating protocol includes: 1. Preparing Acid Washed Glass Cover slips 2. Preparation of Squeaky-Clean Coverslips 3. Preparation of poly-lysi ...

General guidance on tissue cell culture new review
An excellent guidance on tissue cell culture from Marimoto lab at Northwestern University. (7 pages of PDF file) ...

Hydrogel cell culture new review
Hydrogel is the three-dimensional synthetic gels that can be used in cell culture to mimic the extracellular matrix for cell interactions studies. Bioengineers are moving steady forward in recapitulating endogenouse factors in tissue to make hydrogels with ch ...

DSMZ German Collections of Microorganisms and Cell Cultures software
Catalogues: Microorganisms Plant Cell Lines Plant Viruses Human and Animal Cell Lines ...

More cell culture protocols

Basic techniques in mammalian cell tissue culture.
(Curr Protoc Cell Biol. 2007 Sep;Chapter 1:Unit 1.1)
Phelan MC.
Molecular Pathology Laboratory Network, Maryville, Tennessee, USA.

Cultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with the appropriate characteristics, passaging, freezing and storage, recovering frozen stocks, and counting viable cells.


(Selected reviews on cell culture techniques)
1: Seth G, Hossler P, Yee JC, Hu WS.
Engineering cells for cell culture bioprocessing--physiological fundamentals.
Adv Biochem Eng Biotechnol. 2006;101:119-64.

2: van Noort JM.
Human glial cell culture models of inflammation in the central nervous system.
Drug Discov Today. 2006 Jan;11(1-2):74-80.

3: Seth P, Major EO.
Human brain derived cell culture models of HIV-1 infection.
Neurotox Res. 2005 Oct;8(1-2):83-9.

4: Iwaguro H, Asahara T.
Endothelial progenitor cell culture and gene transfer.
Methods Mol Med. 2005;112:239-47.

5: Dimri G, Band H, Band V.
Mammary epithelial cell transformation: insights from cell culture and mouse
models.
Breast Cancer Res. 2005;7(4):171-9. Epub 2005 Jun 3.

6: Forbes B, Ehrhardt C.
Human respiratory epithelial cell culture for drug delivery applications.
Eur J Pharm Biopharm. 2005 Jul;60(2):193-205. Epub 2005 Apr 21.

7: Gudjonsson T, Villadsen R, Ronnov-Jessen L, Petersen OW.
Immortalization protocols used in cell culture models of human breast
morphogenesis.
Cell Mol Life Sci. 2004 Oct;61(19-20):2523-34.

8: Ishikawa M, Asahara T.
Endothelial progenitor cell culture for vascular regeneration.
Stem Cells Dev. 2004 Aug;13(4):344-9.

9: Arden N, Betenbaugh MJ.
Life and death in mammalian cell culture: strategies for apoptosis inhibition.
Trends Biotechnol. 2004 Apr;22(4):174-80.

10: MacLeod KG, Langdon SP.
Essential techniques of cancer cell culture.
Methods Mol Med. 2004;88:17-29.

11: Langdon SP.
Basic principles of cancer cell culture.
Methods Mol Med. 2004;88:3-15.

12: Korke R, Rink A, Seow TK, Chung MC, Beattie CW, Hu WS.
Genomic and proteomic perspectives in cell culture engineering.
J Biotechnol. 2002 Mar 14;94(1):73-92.

13: Stacey G, Viviani B.
Cell culture models for neurotoxicology.
Cell Biol Toxicol. 2001;17(4-5):319-34.

14: Sambruy Y, Ferruzza S, Ranaldi G, De Angelis I.
Intestinal cell culture models: applications in toxicology and pharmacology.
Cell Biol Toxicol. 2001;17(4-5):301-17.

15: Battle T, Stacey G.
Cell culture models for hepatotoxicology.
Cell Biol Toxicol. 2001;17(4-5):287-99.

16: Laken HA, Leonard MW.
Understanding and modulating apoptosis in industrial cell culture.
Curr Opin Biotechnol. 2001 Apr;12(2):175-9.

17: Crane MS.
Mutagensis and cell transformation in cell culture.
Methods Cell Sci. 1999;21(4):245-53.

18: Lubiniecki AS.
Elimination of serum from cell culture medium.
Dev Biol Stand. 1999;99:153-6.

19: Helmrich A, Barnes D.
Animal cell culture equipment and techniques.
Methods Cell Biol. 1998;57:3-17.

20: al-Rubeai M, Singh RP.
Apoptosis in cell culture.
Curr Opin Biotechnol. 1998 Apr;9(2):152-6.

21: Ropke C.
Thymic epithelial cell culture.
Microsc Res Tech. 1997 Aug 1;38(3):276-86.

22: Hu WS, Aunins JG.
Large-scale mammalian cell culture.
Curr Opin Biotechnol. 1997 Apr;8(2):148-53.

23: Pollard JW.
Basic cell culture.
Methods Mol Biol. 1997;75:1-11.

24: Reiter M, Bluml G.
Large-scale mammalian cell culture.
Curr Opin Biotechnol. 1994 Feb;5(2):175-9.

cell culture contamination help
(cell culture forum)
help on tissue culture contamination problem

Books on cell and tissue culture methods
(Biowww bookshelf)
Cell and tissue culture techniques

Figure. Stages in the establishment of a cell culture
(Molecular Cell Biology)
6. Manipulating Cells and Viruses in Culture 6.2. Growth of Animal Cells in Culture

Table. Composition of a Typical Medium Suitable for the Cultivation of Mammalian Cells
(Molecular Biology of the Cell )
III. Methods 8. Manipulating Proteins, DNA, and RNA Isolating Cells and Growing Them in Culture

Table. Some Commonly Used Cell Lines
(Molecular Biology of the Cell )
III. Methods 8. Manipulating Proteins, DNA, and RNA Isolating Cells and Growing Them in Culture

Silicon 3t3 culture
(Cell biology forum)
3t3 mouse fibroblast culture

EGF culture supplement question
(Cell biology forum)
epithelial cell culture media need to be supplemented by EGF

HUVEC cell culture
(Cell biology forum)
specialized endothelial enrichment media

HepG2 contamination help
(cell biology forum)
previously stored stable cell lines of HepG2 cells

Cycloheximide: positive control
(cell biology forum)
blocking protein synthesis

Cell culture contamination
(Forum)
tissue culture contamination discussion

culture condition for CHO cell line
(Forum)
CHO cell culture condition

Cell culture and cytokine
(Forum)
Cell culture troubleshooting

How to culture stable cell line?
(Forum)
Stable cell line culture


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