Home / Molecular Biology / Site-directed mutagenesis
| Site-directed mutagenesis is a molecular biology technique in which a mutation is created at a defined site in a DNA molecule, usually a circular molecule known as a plasmid. |
Dut/ung in vitro Site-directed Mutagenesis Protocol
protocol
Protocol from Bjorkerman lab at CIT. Originally written by M. Davis and P. Bjorkman. ...
Some commercial kits and their brief introduction (biocompare)
protocol
Several commercially available site-directed mutagenesis kits and their brief introduction re given in biocompare.com web site. ...
Site-directed Mutagenesis using PCR
protocol
From: Molecular Biology: Current Innovations and Future Trends. Eds. A.M. Griffin and H.G.Griffin. ISBN 1-898486-01-8 1995 ...
QuikChange(tm) Site-Directed Mutagenesis Kit
protocol
Stratagene's QuickChange kit manual. ...
DpnI mediated site-directed Mutagenesis
protocol
Protocol from Nonet lab at Washington University at St. Louise.
A highly effective simple method for making site directed lesions in plasmids without subcloning based on the work of Fisher and Pei (1997).
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General protocol on construction of mutants, chimeras and truncated recombinant plasmid
protocol
A general overview with some technical details on construction of mutant, chimeras and truncated version of plasmid. (from Liz Greene and Steve Henikoff, Kenesin web site) ...
TAMS technology for simple and efficient in vitro site-directed mutagenesis and mutant screening
review
This original article is accessible free from NAR.
Nucleic Acids Research, 2003, Vol. 31, No. 3 e11
TAMS technology for simple and efficient in vitro site-directed mutagenesis and mutant screening
Lei Young* and Qihan Dong
Department of Medic ...
Site-directed mutagenesis
troubleshooting
I have a cloned gene. I want to mutate a single base-pair. Can anyone recommend any good protocol. Which kit do you suggest?
Malay
...
Internal deletions by PCR
troubleshooting
I wonder if anybody could tip me on a good and fast way of making internal deletions in a plasmid by PCR. I have previously done it by introducing new restriction sites by site directed mutagenesis (and then digesting and re-ligating) which takes quite a long ...
Site directed mutagenesis
troubleshooting
help for site directed mutagenesis.
help for site directed mugagenesis ...
Mutagenesis for deletions
troubleshooting
Mutagenesis for deletions w/o kit.
Source of method ...
More site-directed mutagenesis protocols
(Human Molecular Genetics 2)
9. Instability of the human genome: mutation and DNA repair 9.2. Simple mutations
Last update: 
02-Feb-2012 10:33 am
