Technique / Proteomics and protein biochemistry / Chromatography HPLC purification

FPLC fast performance liquid chromatography protocol new protocol
The FPLC consists of a pump and a column which will withstand high pressure so separations can be carried out relatively quickly. For a detailed description there is a FPLC system handbook which is particularly useful for trouble shooting. ...

Protocol for Packing a Reversed-Phase Microcapillary Column protocol
By Hookeun Lee, Proteomecenter.org. ...

MBP-fusion protein chromatography purification protocol version 2.0 new protocol
A very detailed protocol of using chromatography and FPLC to purify MBP-fusion protein in pdf file written by Ken Johnson, Center for Eukaryotic Structural Genomics, Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, Wisco ...

The Chrom-Ed Chromatography Series new recommended review
An online chromatography book edited by Dr. R. P. W. Scott. Content: Principles and Practice of Chromatography Introduction The Development Process Chromatography Nomenclature Factors Controlling Retention Factors Affecting the Magnitude ...

High Performance Liquid Chromatography HPLC: A Users Guide new review
A user guide on HPLC from Analytical Spectroscopy Research Group, Advanced Science and Technology Center at University of Kentucky. Content: About this Guide Applications Bibliography History Manufacturers Technical Assistance T ...

metall ion affinity purification of proteins troubleshooting
are there other (competitive/better) methods for purifying proteins using affinity to chelated metal ions than his6 / nickel? ... ... ...

source for simple chromatography columns troubleshooting
Could anyone please point me to a cheap source for plasic (poly ethylene or similar) columns suitable for 1 to 5 ml of charged agarose? ... ... ...

HPLC Trouble Shooting troubleshooting
HPLC Trouble Shooting guide from Thomson Instrument Company. Abnormal Peak Shape Fluctuation in Elution time Fluctuation in Baseline Noise in Baseline Rise in Pressure ... ... URL: ...

HPLC Interactive troubleshooting guide from Varian new troubleshooting
Varian inc. Troubleshooting include: Problems with Baselines Problems with Clogged Tubing Problems with Leaks Problems with Peaks Problems with Pressure Column Use &Care Data Column Washing Sorbent Specifications ...

An interactive troubleshooting guide on High Performance Liquid Chromatography troubleshooting
Some covered problems: 1.Peaks Broad peaks Ghost peaks Negative peaks Peak doubling Peak fronting Peak tailing Spikes 2.Leaks Leak at column or fittings Leak at detector Leak at injection valve Leak at pump 3.Recovery Poor sample recovery ...

Downloadable HPLC Troubleshooting troubleshooting
From MACHEREY-NAGEL Inc. Content: chapter 1 broad peaks fronting tailing chapter 2 double peaks negative peaks spikes ghost peaks chapter 3 lack of sensitivity poor sample recovery high back pressure ...

LC Troubleshooting guide troubleshooting
From VWR international Ltd. ...

HPLC troubleshooting guide from Waters troubleshooting
This is a 68 pages PDF troubleshooting brochure from Waters. ...

HPLC Troubleshooting Guide from Supelco. troubleshooting
20 pages PDF troubleshooting guide from Supleco, Sigma-Aldrich. ...

Chromatography Equations software
Some useful general, GC, TLC equations. (from VMR interanational) ...

pKa of Common acids and Common Bases software
pKa of Common acids and Common Bases (from VMR international). ...

Free webcast of HPLC Troubleshooting from PE media
Webcast supplied by PE. You will have to register to access the webcasting content. HPLC Troubleshooting (Part 2 of 2*): Recognizing diagnostic clues and interpreting chromatographic results Duration: 117 minutes ...

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Antibody Purification by Affinity Chromatography Protocol
()
Affinity Chromatography Protocol for Anitbody Purification.

HPLC technique books
(Biowww Bookshelf)
High performance liquid chromatography books

Chromatography books
(Biowww Bookshelf)
Chromatographical protein purification

Problem with RP-HPLC purification of peptide
(Forum)
RP-HPLC purification of 22 aa peptide synthesized by solid-phase synthesis.