Technique / Molecular Biology / Saccharomyces cerevisiae Yeast protocol

Large-scale yeast transformation protocol
Protocols were developed by Petra Ross-Macdonald for Mike Snyder's LacZ fusion project at Yale University. (from YGAC Yale Genome Analysis Center) 1. Preparing the competent cells 2. Transforming yeast 3. Equipment required ...

Cloning by co-transformation and recombination in yeast new protocol
Cloning by co-transformation and recombination in yeast protocol. (Dept. Biochemistry, University of Washington) ...

Buffer medium recipes for fission yeast culture protocol
A buffer and medium recipe for fission yeast culture and other lab studies. ...

General yeast RNA protocols new protocol
Web supplementary materials (detailed method) for Genome-wide analysis of mRNA lengths in Saccharomyces cerevisiae. (Evan et.al, Standford University). Content: Total RNA preparation Isolation of poly(A) mRNA from total RNA using a Qiagen Oligote ...

Links to Yeast Protocols on the Web new recommended site
Yeast resources at Duke University. This web site provides comprehensive information about these genome collections, information and protocols for working with S. cerevisiae, links to important yeast related web sites and databases, and links to Duke labs that ...

Yeast Genetics, Biochemistry and Cell Biology site
An valuable site hosted by Herskowitz Lab. It includes lists of readings, links and original protocols on yeast genetics, cell biology and biochemistry. ...

Yeast nucleus staining troubleshooting
I am currently using DAPI to visualize yeast nuclei, but the signal from nucleus is often obscured by strong signal from mitochondria. So I am thinking about using Hoechst 33258 or 33342. Could someone give me some info what is the difference between ...

S. pombe work FAQ troubleshooting
This FAQ answers the most frequently asked questions about working with S. pombe. (from Forsburg lab at University of south california). ...

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