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Desalting, Concentration, and Buffer Exchange by Dialysis and Ultrafiltration
recommended
protocol
Current protocols in protein science. This is a free sample chaptor which is fully accessible. (chaptor by Allen T. Phillips,
Pennsylvania State University) ...
Effective desalting and concentration of in-gel digest samples
new
protocol
Effective desalting and concentration of in-gel digest samples with Vivapure? C18 Micro spin columns prior to MALDI-TOF analysis. By A. Kocourek, M. Naldrett and R. Zeidler. Vivascience AG, Hanover, Germany.
It is well known that the success of protein iden ...
Desalting Protocol for oligos
protocol
Desalting Protocol (for 200 nm scale oligos).
Protcol from Molecular structures facility, UC Davis. ...
Methanol-Chloroform Precipitation of Proteins
new
protocol
A very short protocol on methanol chloroform precipitation of proteins for concentrate and desalt proteins. ...
Brad Olson's Protein Assay Resource
recommended
review
This is a general resource for the commonly used protein assay methods. (Bradley J. S. C. Olson, University of Nebraska-Lincoln).
Assays:
Ultraviolet Absorbance
The Bradford Assay
The Lowry Assay
The BCA Assay ...
LabDialysisChat.com: The Answers for All of Your Laboratory Dialysis Questions
site
An active web forum discussing protein dialysis. A good place to go if you have dialysis problems. ...
Guidline on protein sample preparation for crystallization study
troubleshooting
Guidline on protein sample preparation for crystallization study.
1. Enzymatic or functional assay.
2. Purity of the protein sample.
3. Sample homogeneity.
4. Protein concentration and starting buffer.
From x-tal protcols by Johan Zeelen. ...
What is the most effective way to concetrate protein samples whilst removing excess salt?
troubleshooting
A forum thread on concentration and desalting of protein samples. (From NWFSC forum archive) ...
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