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| Methods and protocols on cloning vectors including bacterial phage, plasmid, and bacterial host strains. |
Protein expression in E.Coli
protocol
This protocol described a strategy of parallel expression of a protein from a variety of vectors containing different tags and/or fusion partners, and a variety of E. coli host strains.
The strategy to expression recombinant protein in e.coli involves two ...
Lambda protocol
protocol
Protocol from Rob Phillips Group.
The well-known lambda bacteriophage grows on E. coli bacteria. Here's what you need to know to work with it.
Preparation of plates with rich medium
Preparation of liquid medium
Titering lambda
TM buffer for storage ...
Bacterial cell culture
protocol
Bacterial cell culture general procedures:
Follow microbiology bacterial handling practice for all procedures.
- Streak agar plate from bacterial stock and grow overnight at 37c bacterial incubator.
- Transfer bacterial colony using sterile toothpick ...
Generally useful protocols for phage and bacteria
protocol
Here are some generally useful protocols for phage and bacteria.
Preparation of plates with rich medium
Preparation of liquid medium ...
Bacteriology protocol
protocol
PDF protocol for bacterial growing, storage and medium recipe etc. (From Marimoto lab at Northwestern University). ...
Prepare competent cells with RbCl2 method
protocol
PDF protocol from Marimoto lab at Northwestern University.
The procedure can be used to obtain competent cells with a transformation frequency of 10^6 - 10^7 colonies per microgram of DNA. Conveniently, these cells can be stored for months with relatively ...
Preparation of E.Coli competent cells with CaCl2 method
protocol
Preparation of E.Coli competent cells with CaCl2 method protocol from Marimoto lab at NothWestern University. (PDF file). ...
E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology)
review
E. Coli Plasmid Vectors: Methods and Applications (Methods in Molecular Biology (Clifton, ...
E.coli recombinant protein proteolysis
troubleshooting
We've been trying to express a pro-hormone as a GST fusion in E. coli
(DH5-alpha) but seem to be having problems with proteolysis - we think this
may be occuring at double basic sites but are not completely sure.
... ...
Click following links ...
need help for the transformation
troubleshooting
I want to construct a DNA library with different sequence. First step,
PCR, the template contained random 40mers in the middle and normal
sequence on each end. After the PCR, I could obtain the PCR product
library and I ligated them into the plasmid v ...
Best vector for expressing recombinant antibody fragments?
troubleshooting
Does anyone here express recombinant antibody fragments regularly in
their research? Which vector do you use and do you have to refold? I am
looking for one that gives me the highest yields (I know this depends on
individual sequences as well) and ...
Protocol for making competent E coli
troubleshooting
Does anyone have a quick and simple way of making competent E coli for
transformation (heat shock)? ... ...
...
Plasmid or phagemid vector(s) holding large insert
troubleshooting
I am looking for plasmid or phagemid vector(s) which can hold 50kb DNA insert. I
appreciate it very much if anybody can send me information about the name of the plasmid and the place I can get it ... ...
...
Transformation efficiency
troubleshooting
If you are getting low transformation efficiency with chemically competent cells:
There were impurities in the DNA. Remove phenol, protein, detergents, and ethanol, by ethanol precipitation or GlassMAX DNA Isolation Systems. There was excess DNA. Use no mo ...
Glycerol stock longevity
troubleshooting
Does any one have information (data or opinion or personal experience) on
how
long a bacterial glycerol stock is viable @ -80oC? How often to stocks fail
to
regrow? Is is necessary to thaw and revitalize the stock at time intervals?
...
E.Coli strain database (searchable)
database
Large collection of E.Coli strains.
Large collection of E.Coli strains ...
PlasMapper Version 1.0 plasmid maps
software
The PlasMapper server automatically generates and annotates plasmid maps using only the plasmid DNA sequence as input. Plasmid sequences up to 20,000 bp may be annotated and displayed. Plasmid figures may be rendered in PNG, JPG, SVG or SVGZ format. PlasMapper ...
More recombinant dna protocols
Last update: 
01-Feb-2012 04:17 am
