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Technique / Genetics / Genetic Screening / PCR amplification of specific alleles (PASA)


Mutation detection using allele-specific polymerase chain reaction protocol
Polymerase chain reaction (PCR) amplification of specific alleles allowed the rapid detection of a point mutation (missense Gly528 -> Asp) in exon 11 of the low density lipoprotein receptor gene which was otherwise not detectable by exon amplification and enzy ...



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