| 1 | Dissolve 186.1g Na2EDTA.2H2O in 800 ml distilled. |
| 2 | pH to 8.0 with NaOH (~20g of NaOH pellets). EDTA will dissolve at pH 8.0. |
| 3 | Adjust volume to 1 liter with distilled water. |
| 4 | Sterilize by autoclaving and store at room temperature. |
Laboratory media reagent and buffer recipes
| 1 | Dissolve 186.1g Na2EDTA.2H2O in 800 ml distilled. |
| 2 | pH to 8.0 with NaOH (~20g of NaOH pellets). EDTA will dissolve at pH 8.0. |
| 3 | Adjust volume to 1 liter with distilled water. |
| 4 | Sterilize by autoclaving and store at room temperature. |