| 1 | Dissolve 15 G PIPES, 1.9 g EGTA, 1.32 g MgSO4¡¤7H2O and 5 g KOH in 800 ml distilled water. |
| 2 | Adjust to pH 7.0 with KOH. Make up volume to 1 liter with distilled water. |
| 3 | Sterilize by autoclaving. |
Laboratory media reagent and buffer recipes
| 1 | Dissolve 15 G PIPES, 1.9 g EGTA, 1.32 g MgSO4¡¤7H2O and 5 g KOH in 800 ml distilled water. |
| 2 | Adjust to pH 7.0 with KOH. Make up volume to 1 liter with distilled water. |
| 3 | Sterilize by autoclaving. |