| 1 | 41.8 g MOPS |
| 2 | in 700 ml of sterile DEPC-treated H2O. |
| 3 | Adjust the pH to 7.0 with 2 N NaOH. |
| 4 | Add 20 ml of DEPC-treated 1 M sodium acetate |
| 5 | 20 ml of DEPC-treated 0.5 M EDTA (pH 8.0). |
| 6 | Adjust the volume to 1 liter with DEPC-treated H2O. |
Laboratory media reagent and buffer recipes
| 1 | 41.8 g MOPS |
| 2 | in 700 ml of sterile DEPC-treated H2O. |
| 3 | Adjust the pH to 7.0 with 2 N NaOH. |
| 4 | Add 20 ml of DEPC-treated 1 M sodium acetate |
| 5 | 20 ml of DEPC-treated 0.5 M EDTA (pH 8.0). |
| 6 | Adjust the volume to 1 liter with DEPC-treated H2O. |