2x YT Media, pH 7.0
In one liter bottle add:
900 ml distilled H2O
16g Bacto Tryptone
10g Bacto Yeast Extract
5 g Sodium chloride (NaCl)
Adjust pH to 7.0 with 5N NaOH
Adjust to 1L with distilled H2O
Sterilize by autoclaving.