Luria Broth Buffer
1 Dissolve 25 g Luria broth powder in 750 ml of distilled water.
2 Add 800 ml distilled water.
3 Adjust to pH 7.0 with either HCl or NaOH as appropriate.
4 Make up volume to 1 liter with distilled water.
5 Sterilize by autoclaving.