| 1 | Add 1 g NH4Cl and 3 g glucose in 100 ml 10X M9 Salts salution. |
| 2 | Add 1 ml 1 M MgSO4 and 10 ml Gibco MEM vitamin solution. |
| 3 | Make up volume to 1 liter with distilled water. |
| 4 | Check that the pH is 7.0-7.4. |
| 5 | Filter sterilize through a 0.22 micron filter. |
| 6 | Add 1 ml sterile 100 mM CaCl2 solution. Store at room temperature. |